产品说明
DescriptionApplicationReferencesDataS.D.SProduct DescriptionCytoRed is cell membrane permeable and accumulates inside of viable cells as resorufin (Fig. 1). CytoRed has a much wider spectrum than BCECF or Calcein, so filters for fluorescein and rhodamine can also be used. The excitation and emission wavelengths of resorufin are 560 nm and 590 nm, respectively. A photo of cells stained with CytoRed is shown in Fig. 2.Fig. 1 Cell staining mechanismStaining Procedure1.Prepare 1 mM CytoRed solution with DMSO. Dilute it to prepare 10 μM CytoRed solution with culture medium or an appropriate buffer.a)2.Prepare a 1×105-1×106 cells/ml cell suspension and culture the cells in a chamber slide.3.Remove culture medium and wash cells with culture medium (PBS-Hanks medium, etc).4.Add CytoRed solution to the cells, and incubate the chamber at 37ºC for 30 min to 1 hour.5.Remove the culture medium from cells and add new medium.b)6.Wash cells twice with PBS or an appropriate buffer.7.Observe the cells under a fluorescence microscope with 560 nm excitation and 590 nm emission filters.a) Incubate the MitoRed buffer solution at 37ºC prior to adding to cells.b) For fixing after washing cells, add 10% formarin buffer and incubate for 15-20 min, and then wash with PBS.1. M. Ishiyama, et al., A Resorufin Derivative as a Fluorogenic Indicator for Cell Viability. Anal Sci. 1999;15:1025-1028.Staining DataFig. 2 Cell staining with CytoRedCell type: HeLa
Dojindo细胞分析
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。
细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢
| 应用 | 产品展示 |
| 细胞生长检测，药物筛选，比色/荧光检测 | 细胞计数试剂盒-8 细胞计数试剂盒8 + 96孔有机硅定向剂 细胞计数试剂盒-F 细胞毒性LDH检测试剂盒 -WST 96孔有机硅定向剂 MTT |
| 了解检测机制的差异： | 点击这里 |
| 细胞周期分析 | 细胞周期测定溶液深红色 细胞周期测定溶液蓝色 |
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