产品说明
DescriptionApplicationReferencesDataS.D.SProduct DescriptionFDA is cell-membrane permeable and accumulates inside of viable cells as fluorescein (Fig. 1). Since fluorescein is less hydrophilic than BCECF or Calcein, the leakage of fluorescein from cells is rather high. FDA is also utilized for flow cytometry. The excitation and emission wavelengths of fluorescein are 488 nm and 530 nm, respectively. FDA-stained cells are shown in Fig. 2.Fig. 1 Cell staining mechanismStaining Procedure1.Prepare 0.5 mg/ml FDA stock solution with DMSO. Dilute 10 ul of the stock solution with 5 ml PBS(-).2.Prepare a cell suspension and wash cells with PBS(-). Prepare 1×105-1×106 cells/ml cell suspension3.Add 15 ul FDA solution to 30 ul cell suspension, and incubate at 37ºC for 15-30 min.4.Put 10 ul stained cell suspension on a glass slide and cover with a cover glass.5.Observe the cells under a fluorescence microscope with 488 nm excitation and 530 nm emission filters.1. B. Rotman, et al., Membrane Properties of Living Mammalian Cells as Studied by Enzymatic Hydrolysis of Fluorogenic Esters. PNAS. 1966;55:134-141.2. H. R. Hulett, et al., Cell Sorting: Automated Separation of Mammalian Cells as a Function of Intercellular Fluorescence. Science. 1969;166:747-749.3. K. H. Jones, et al., An Improved Method to Determine Cell Viability by Simultaneous Staining with Fluorescein Diacetate-Propidium Iodide. J Histochem Cytochem. 1985;33:77-79.4. K. McGinnes, et al., A Fluorescence NK Assay Using Flow Cytometry. J Immunol Methods. 1986;86:7-15.5. W. M. J. Vuist, et al., Potentiation by Interleukin 2 of Burkitt’s Lymphoma Therapy with Anti-Pan B (Anti-CD19) Monoclonal Antibodies in a Mouse Xenotransplantation Model. Cancer Res. 1989;49:3783-3788.6. E. Prosperi, Intracellular Turnover of Fluorescein Diacetate. Influence of Membrane Ionic Gradients on Fluorescein Efflux. Histochem J. 1990;22:227-233.Fig. 2 Cell staining with FDA,Cell type: HeLaRelated Categories Cell Staining
Dojindo细胞分析
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。
细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢
| 应用 | 产品展示 |
| 细胞生长检测，药物筛选，比色/荧光检测 | 细胞计数试剂盒-8 细胞计数试剂盒8 + 96孔有机硅定向剂 细胞计数试剂盒-F 细胞毒性LDH检测试剂盒 -WST 96孔有机硅定向剂 MTT |
| 了解检测机制的差异： | 点击这里 |
| 细胞周期分析 | 细胞周期测定溶液深红色 细胞周期测定溶液蓝色 |
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