产品说明
DescriptionApplicationReferencesDataS.D.SProduct DescriptionHoechst dyes are cell membrane permeable and stain DNA to emit intense blue fluorescence. They bind to DNA in the minor groove of poly-AT sequence rich areas. Both Hoechst 33342 and Hoechst 33258 are water-soluble and stable in aqueous solutions. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively.Chemical StructureStaining Procedure1.Prepare 10-50 μM Hoechst dye solution with PBS or an appropriate buffer.a)2.Add Hoechst dye solution with 1/10 of the volume of cell culture medium to the cell culture.b)3.Incubate the cell at 37ºC for 10-20 min.4.Wash cells twice with PBS or an appropriate buffer.5.Observe the cells under a fluorescence microscope with 350 nm excitation and 460 nm emission filters.a) Since Hoechst dyes may be carcinogenic, extreme care is necessary during handling.b) Or you may replace the culture medium with 1/10 concentration of Hoechst dye buffer solution.1. M. J. Lydon, et al., Vital DNA Staining and Cell Sorting by Flow Microfluorometry. J Cell Physiol. 1980;102:175-181.2. M. Sriram, et al., Structural Consequences of a Carcinogenic Alkylation Lesion on DNA: Effect of O6-ethylguanine on the Molecular Structure of the d(CGC[e6G]AATTCGCG)-netropsin Complex. Biochemistry. 1992;31:11823-11834.3. Y. Tadokoro, et al., Characterization of Histone H2A.X Expression in Testis and Specific Labeling of Germ Cells at the Commitment Stage of Meiosis with Histone H2A.X Promoter-Enhanced Green Fluorescent Protein Transgene. Biol Reprod. 2003;69:1325-1329.4. F. Wada, et al., Analyses of Expression and Localization of Two Mammalian-Type Transglutaminases in Physarum polycephalum, an Acellular Slime Mold. J Biochem. 2004;136:665-672.5. T. Ohara, et al., FoSTUA, Encoding a Basic Helix-Loop-Helix Protein, Differentially Regulates Development of Three Kinds of Asexual Spores, Macroconidia, Microconidia, and Chlamydospores, in the Fungal Plant Pathogen Fusarium oxysporum. Eukaryot Cell. 2004;3:1412-1422.Staining DataFig. 2 Cell staining with Hoechst 33258Cell type: human fetal cellFig. 3 Cell staining with Hoechst 33342Cell type: human fetal cellRelated Categories Kits for Life Science Research Cell Staining
Dojindo细胞分析
细胞活力和细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。Dojindo开发了高度水溶性的四唑盐，称为WST。WST-8是高度稳定的WST，用于Cell Counting Kit-8（CCK-8）。由于WST-8甲maz是水溶性的，因此不会形成晶体。因此，不需要诸如MTT测定的增溶过程。此外，CCK-8的检测灵敏度高于其他四唑盐，例如MTT，XTT，MTS或WST-1。
细胞增殖/细胞毒性转染细胞染色细胞内荧光探针细菌染色微生物活力测定干细胞分化SPiDER-ßGal线粒体检测细胞代谢
| 应用 | 产品展示 |
| 细胞生长检测，药物筛选，比色/荧光检测 | 细胞计数试剂盒-8 细胞计数试剂盒8 + 96孔有机硅定向剂 细胞计数试剂盒-F 细胞毒性LDH检测试剂盒 -WST 96孔有机硅定向剂 MTT |
| 了解检测机制的差异： | 点击这里 |
| 细胞周期分析 | 细胞周期测定溶液深红色 细胞周期测定溶液蓝色 |
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