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Glen research/Ac-dC-CE Phosphoramidite/1kit/10-1015-02M
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Technical DocumentsDescriptionDetailsDilution/Coupling DataRelated ProductsProduct FAQsTechnical DocumentsSafety Data SheetGlen Report 11.25: Alternatives to Expedite MonomersGlen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNAGlen Report 26.14: Technical Brief - AMA – Ubiquitous Reagent for Oligo DeprotectionGlen Report 27.13: Technical Brief - APA: An Alternative to AMA DeprotectionGlen Report 6.22: UltraFast DNA Synthesis - 10 Minute DeprotectionGlen Report 7.12: UltraFast ChemistryDescriptionGlen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.DetailsUsageCoupling: No changes needed from standard method recommended by synthesizer manufacturer.Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.SpecificationsDiluentAnhydrous AcetonitrileStorageControlled room temperature or lower, dryStabilitySimilar to dA,C,G,T-CE PhosphoramiditesDilution/Coupling DataThe table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.ABI 392/394Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of AdditionsLV40LV20040nm0.2μm1μm10μm10-1015-020.25 g.25grams3.2494.6756.835.525.8218.934.7310-1015-050.5 g.5grams6.48202.67121.67655.2740.5310.1310-1015-101.0 g1grams12.96418.67251.2157114.1883.7320.9310-1015-202.0 g2grams25.91850.33510.2318.88231.91170.0742.5210-1015-404.0 g4grams51.8217141028.4642.75467.45342.885.7ExpediteCatalog #Pack SizeGrams/PackDilution (mL)Approximate Number of AdditionsMolarity50nm0.2μm1μm15μm10-1015-C20.25 g.25grams4.830.0793.458.3842.455.8410-1015-C50.5 g.5grams9.670.07190.2118.8886.4511.8910-1015-1C1.0 g1grams19.340.07383.6239.75174.3623.9810-1015-2C2.0 g2grams38.670.07770.2481.38350.0948.14Related Products/*.carousel-inner .active.left{ left: -33%; }*//*.carousel-inner .active.right { left: 33%;}*//*.carousel-inner .next { left: 33% }*//*.carousel-inner .prev { left: -33%}*//*.carousel-control.left{ background-image: none; position: relative; float: left; }*//*.carousel-control.right { background-image: none; position: relative; float: right;}*//*.carousel-inner .item { background: white;}*//*.block.upsell {display: none; }*//*.products .product-item-details {*//*padding-left: 10px;*//*padding-right: 10px;*//*background: #efeeee;*//*color: #58595b;*//*font-size: 18px;*//*padding: 20px 10px;*//*border-radius: 0 0 3px 3px; }*//*.owl-wrapper-outer{*//*overflow: hidden;*//*position: relative;*//*width: 100%;*//*}*//*.block.related .owl-carousel .owl-item {*//*padding: 0px 7px;*//*}*/Related Products Ac-dC-CPG 500Catalog #: 20-2013Ac-dC-CPG 1000Catalog #: 20-2015Pac-dA-CE PhosphoramiditeCatalog #: 10-1601iPr-Pac-dG-CE PhosphoramiditeCatalog #: 10-1621dT-CE PhosphoramiditeCatalog #: 10-1030dC-CE PhosphoramiditeCatalog #: 10-1010Product FAQsHow does this work?What would happen if I used the AMA reagent with Bz protected dC?dC when protected as an amide linkage is susceptible to transamination or displacement of the amide with the deprotecting amine.Normally, the deprotecting amine is ammonia and displacement leads to the same product as hydrolysis.However, if methylamine is used for the deprotection step with a benzoyl protected dC, approximately 10% of the product is from the displacement reaction to give N-methyl-dC residues, while the remaining 90% is the desired product formed by hydrolysis.A more labile protecting group on dC is isobutyryl which is also commercially available.This group is displaced by methylamine to about 5% - an improvement but still a mutation.Ac-dC, on the other hand, is hydrolyzed virtually instantaneously by the AMA reagent and none of the slower displacement reaction gets a chance to occur.||REFERENCE(S):M.P. Reddy, Beckman Instruments, personal communication.||Can the AMA reagent be made up and stored? for how long? in what kind of container?Yes.Store tightly sealed in a glass bottle in the refrigerator.The reagent does not decompose but routine opening will lose some of the volatile bases.Dispose properly after 4-6 weeks.||REFERENCE(S):Candy Lee, Dartmouth.catalog-product-faqs{width: 100%;}.faq_list .faqItem .faqTitle::before {content: "e250";font-family: "Glyphicons Halflings";font-size: 14px;right: 18px;position: absolute;top: 30%;-moz-transform: rotate(0);-webkit-transform: rotate(0);transform: rotate(0);-moz-transition: all ease-in-out 0.2s;-webkit-transition: all ease-in-out 0.2s;transition: all ease-in-out 0.2s;}#catalog_custom_product_faqs{width: 100%;}
Glen research对我们的员工,我们的客户和环境的承诺
Maravai LifeSciences致力于在所有业务活动中成为环境,健康与安全(EHS)管理方面的领导者,
符合Maravai LifeSciences的商业利益和行为准则。
我们承诺:
雇员
我们将通过提供零伤害的工作场所来预防伤害和疾病
并促进健康的生活方式,从而对每位员工产生积极影响。我们将确保我们的员工具有
执行此政策的意识,工具和知识。
可持续发展
我们将通过使用确保安全,效率和社会责任的商业惯例,以
现在和将来保护我们的员工,社区,股东和环境的方式,进行可持续思考和采取行动。
合规
我们将遵守适用的法律和法规,同时提供程序和程序以确保我们的遵守。
文化
我们将通过停工授权,
查明根本原因并采取纠正措施,倡导一种不容忍不安全状况和行为的文化。
业务整合
我们会将环境,健康和安全考虑因素纳入我们的业务活动。
顾客
我们将与客户紧密合作,以帮助他们满足其环境,健康和安全需求。
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我们将与我们的供应商和承包商合作,以改善环境,健康和安全绩效。
社区与政府
我们将参与社区和政府的环境,健康和安全计划。
这些价值观得到了Maravai高层管理人员的重视,并在组织内的所有责任级别得到强调。
卡尔·赫尔(Carl Hull)
首席执行官
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