Technical DocumentsDescriptionDetailsDilution/Coupling DataRelated ProductsProduct FAQsTechnical DocumentsSafety Data SheetGlen Report 11.25: Alternatives to Expedite MonomersGlen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNAGlen Report 26.14: Technical Brief - AMA – Ubiquitous Reagent for Oligo DeprotectionGlen Report 27.13: Technical Brief - APA: An Alternative to AMA DeprotectionGlen Report 6.22: UltraFast DNA Synthesis - 10 Minute DeprotectionGlen Report 7.12: UltraFast ChemistryDescriptionGlen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination and tested to ensure a tight fit on synthesizers.DetailsUsageCoupling: No changes needed from standard method recommended by synthesizer manufacturer.Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.SpecificationsDiluentAnhydrous AcetonitrileStorageControlled room temperature or lower, dryStabilitySimilar to dA,C,G,T-CE PhosphoramiditesDilution/Coupling DataThe table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.ABI 392/394Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of AdditionsLV40LV20040nm0.2μm1μm10μm10-1015-020.25 g.25grams3.2494.6756.835.525.8218.934.7310-1015-050.5 g.5grams6.48202.67121.67655.2740.5310.1310-1015-101.0 g1grams12.96418.67251.2157114.1883.7320.9310-1015-202.0 g2grams25.91850.33510.2318.88231.91170.0742.5210-1015-404.0 g4grams51.8217141028.4642.75467.45342.885.7ExpediteCatalog #Pack SizeGrams/PackDilution (mL)Approximate Number of AdditionsMolarity50nm0.2μm1μm15μm10-1015-C20.25 g.25grams4.830.0793.458.3842.455.8410-1015-C50.5 g.5grams9.670.07190.2118.8886.4511.8910-1015-1C1.0 g1grams19.340.07383.6239.75174.3623.9810-1015-2C2.0 g2grams38.670.07770.2481.38350.0948.14Related Products/*.carousel-inner .active.left{ left: -33%; }*//*.carousel-inner .active.right { left: 33%;}*//*.carousel-inner .next { left: 33% }*//*.carousel-inner .prev { left: -33%}*//*.carousel-control.left{ background-image: none; position: relative; float: left; }*//*.carousel-control.right { background-image: none; position: relative; float: right;}*//*.carousel-inner .item { background: white;}*//*.block.upsell {display: none; }*//*.products .product-item-details {*//*padding-left: 10px;*//*padding-right: 10px;*//*background: #efeeee;*//*color: #58595b;*//*font-size: 18px;*//*padding: 20px 10px;*//*border-radius: 0 0 3px 3px; }*//*.owl-wrapper-outer{*//*overflow: hidden;*//*position: relative;*//*width: 100%;*//*}*//*.block.related .owl-carousel .owl-item {*//*padding: 0px 7px;*//*}*/Related Products Ac-dC-CPG 500Catalog #: 20-2013Ac-dC-CPG 1000Catalog #: 20-2015Pac-dA-CE PhosphoramiditeCatalog #: 10-1601iPr-Pac-dG-CE PhosphoramiditeCatalog #: 10-1621dT-CE PhosphoramiditeCatalog #: 10-1030dC-CE PhosphoramiditeCatalog #: 10-1010Product FAQsHow does this work?What would happen if I used the AMA reagent with Bz protected dC?dC when protected as an amide linkage is susceptible to transamination or displacement of the amide with the deprotecting amine.Normally, the deprotecting amine is ammonia and displacement leads to the same product as hydrolysis.However, if methylamine is used for the deprotection step with a benzoyl protected dC, approximately 10% of the product is from the displacement reaction to give N-methyl-dC residues, while the remaining 90% is the desired product formed by hydrolysis.A more labile protecting group on dC is isobutyryl which is also commercially available.This group is displaced by methylamine to about 5% - an improvement but still a mutation.Ac-dC, on the other hand, is hydrolyzed virtually instantaneously by the AMA reagent and none of the slower displacement reaction gets a chance to occur.||REFERENCE(S):M.P. Reddy, Beckman Instruments, personal communication.||Can the AMA reagent be made up and stored? for how long? in what kind of container?Yes.Store tightly sealed in a glass bottle in the refrigerator.The reagent does not decompose but routine opening will lose some of the volatile bases.Dispose properly after 4-6 weeks.||REFERENCE(S):Candy Lee, Dartmouth.catalog-product-faqs{width: 100%;}.faq_list .faqItem .faqTitle::before {content: "e250";font-family: "Glyphicons Halflings";font-size: 14px;right: 18px;position: absolute;top: 30%;-moz-transform: rotate(0);-webkit-transform: rotate(0);transform: rotate(0);-moz-transition: all ease-in-out 0.2s;-webkit-transition: all ease-in-out 0.2s;transition: all ease-in-out 0.2s;}#catalog_custom_product_faqs{width: 100%;}