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Glen research/Pac-dA-CE Phosphoramidite/1kit/10-1601-10E

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Technical DocumentsDescriptionDetailsDilution/Coupling DataRelated ProductsTechnical DocumentsSafety Data SheetProcedure for UltraMild Deprotection of OligonucleotidesGlen Report 11.25: Alternatives to Expedite MonomersDescriptionAn alternative protecting scheme for the normal CE phosphoramidites should allow UltraMILD deprotection and should not react with a wider variety of tags and labels. A set of monomers using phenoxyacetyl (Pac) protected dA and 4-isopropyl-phenoxyacetyl (iPr-Pac) protected dG, along with acetyl protected dC, met the desired criteria for UltraMILD deprotection.We recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix. Cleavage and deprotection can be carried out in 2 hours at room temperature with ammonium hydroxide or 4 hours with 0.05M potassium carbonate in methanol.DetailsUsageCoupling: No changes needed from standard method recommended by synthesizer manufacturer.To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium HydroxideSpecificationsDiluentAnhydrous AcetonitrileStorageFreezer storage, -10 to -30°C, dryStability24 hoursDilution/Coupling DataThe table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.ABI 392/394Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of AdditionsLV40LV20040nm0.2μm1μm10μm10-1601-020.25 g.25grams2.8280.6748.430.252216.134.0310-1601-050.5 g.5grams5.63174.33104.665.3847.5534.878.7210-1601-101.0 g1grams11.26362217.2135.7598.7372.418.1ExpediteCatalog #Pack SizeGrams/PackDilution (mL)Approximate Number of AdditionsMolarity50nm0.2μm1μm15μm10-1601-020.25 g.25grams4.20.0777.648.535.274.8510-1601-050.5 g.5grams8.40.07161.610173.4510.110-1601-101.0 g1grams16.810.07329.8206.13149.9120.61Related Products/*.carousel-inner .active.left{ left: -33%; }*//*.carousel-inner .active.right { left: 33%;}*//*.carousel-inner .next { left: 33% }*//*.carousel-inner .prev { left: -33%}*//*.carousel-control.left{ background-image: none; position: relative; float: left; }*//*.carousel-control.right { background-image: none; position: relative; float: right;}*//*.carousel-inner .item { background: white;}*//*.block.upsell {display: none; }*//*.products .product-item-details {*//*padding-left: 10px;*//*padding-right: 10px;*//*background: #efeeee;*//*color: #58595b;*//*font-size: 18px;*//*padding: 20px 10px;*//*border-radius: 0 0 3px 3px; }*//*.owl-wrapper-outer{*//*overflow: hidden;*//*position: relative;*//*width: 100%;*//*}*//*.block.related .owl-carousel .owl-item {*//*padding: 0px 7px;*//*}*/Related Products Pac-dA-CPGCatalog #: 20-2601Ac-dC-CE PhosphoramiditeCatalog #: 10-1015iPr-Pac-dG-CE PhosphoramiditeCatalog #: 10-16215% Phenoxyacetic anhydride in THF/PyridineCatalog #: 40-42100.05M Potassium Carbonate in MethanolCatalog #: 60-4600