Technical DocumentsDescriptionDetailsIntellectual PropertyDilution/Coupling DataRelated ProductsTechnical DocumentsSafety Data SheetGlen Report 17.13: UniCap Phosphoramidite, AN alternative to acetic anhydride cappingGlen Report 30.1: Technical SnippetsDescriptionThe phosphoramidite of diethylene glycol monoethyl ether, UniCap, is the basis for an alternative capping reagent.To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument.Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step.The standard capping steps can be left out of the cycle.UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.DetailsUsageCoupling: For capping, add as a second coupling step after base coupling using standard coupling conditions.Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.SpecificationsDiluentAnhydrous AcetonitrileStorageFreezer storage, -10 to -30°C, dryStability7 daysIntellectual PropertyThis product is supplied under license.Dilution/Coupling DataThe table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.ABI 392/394Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of AdditionsLV40LV20040nm0.2μm1μm10μm10-4410-020.25 g.25grams7.48236141.688.564.3647.211.810-4410-050.5 g.5grams14.95485291181.88132.279724.2510-4410-101.0 g1grams29.91983.67590.2368.88268.27196.7349.1810-4410-202.0 g2grams59.811980.331188.2742.63540.09396.0799.02ExpediteCatalog #Pack SizeGrams/PackDilution (mL)Approximate Number of AdditionsMolarity50nm0.2μm1μm15μm10-4410-020.25 g.25grams11.160.07216.8135.598.5513.5510-4410-050.5 g.5grams22.320.0744027520027.510-4410-101.0 g1grams44.630.07886.2553.88402.8255.3910-4410-202.0 g2grams89.270.0717791111.88808.64111.19Related Products/*.carousel-inner .active.left{ left: -33%; }*//*.carousel-inner .active.right { left: 33%;}*//*.carousel-inner .next { left: 33% }*//*.carousel-inner .prev { left: -33%}*//*.carousel-control.left{ background-image: none; position: relative; float: left; }*//*.carousel-control.right { background-image: none; position: relative; float: right;}*//*.carousel-inner .item { background: white;}*//*.block.upsell {display: none; }*//*.products .product-item-details {*//*padding-left: 10px;*//*padding-right: 10px;*//*background: #efeeee;*//*color: #58595b;*//*font-size: 18px;*//*padding: 20px 10px;*//*border-radius: 0 0 3px 3px; }*//*.owl-wrapper-outer{*//*overflow: hidden;*//*position: relative;*//*width: 100%;*//*}*//*.block.related .owl-carousel .owl-item {*//*padding: 0px 7px;*//*}*/Related Products 0.25M DCI in Anhydrous AcetonitrileCatalog #: 30-31506.5% Dimethylaminopyridine in TetrahydrofuranCatalog #: 40-40203% Dichloroacetic acid in DichloromethaneCatalog #: 40-40405-Carboxy-dC-CE PhosphoramiditeCatalog #: 10-10660.5M CSO in Anhydrous AcetonitrileCatalog #: 40-4632