首页 > Epigentek > Epigentek/EpiQuik Nuclear Extraction Kit/OP-0002-1/100 assays

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Epigentek/EpiQuik Nuclear Extraction Kit/OP-0002-1/100 assays

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OP-0002-1

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4000-520-616

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InputType:UnisolatedSamplesResearchArea:DNAMethylation,HistoneAcetylation,HistoneMethylation,SumoylationTargetApplication:SampleIsolationVesselFormat:Columns/Tubes100%Guarantee:6monthsProductOverviewThe EpiQuik™NuclearExtractionKitisacompletesetofoptimizedreagentstoprovideasimpleandselectivemethodforisolatingnuclearproteinsusedforavarietyofapplicationsinjust60minutes.TheseapplicationsmayincludeWesternblot,protein-DNAbindingassays,nuclearenzymeassays,andanyotherproceduresrequiringoptimizednuclearproteins.Theprotocolisfastandeasy-to-use,andalsoisolatesveryabundantyieldsofnuclearextractfrommammaliancellsortissuesamples. ThekitisalsospecificallydesignedtomeettherequirementsofnuclearextractsusedinvariousotherepigeneticproductsfromEpigentek,including DNMT, HDAC,and HAT assays.Someofthekit"shighlightedfeaturesincludethefollowing:Pre-optimizedandsimplestep-wise1hourprotocol.Convenientlyincludesallessentialreagentstocarryoutanuclearextraction.StandardizedprocedureforreproducIBLeresults.Principle&ProcedureThe EpiQuik™NuclearExtractionKitsimplyappliesourproprietarynuclearproteinisolationbufferstocell/tissues.Aftertreatmentwithpre-lysisandlysisbuffers,thenuclearproteinsareeasilyextractedforimmediateuseorstorageatproperconditions.StartingMaterials&YieldAtotalof100standardextractions(using10^7 cellsor20mgtissues)canbeisolatedfrom mammaliancellsortissuesampleswiththiskit.Totalyieldcanbeupto100µgperoptimalextraction,althoughthe resultsmaysomewhatvarydependingonthecellortissuetype.ProductComponentsNE1(10XPre-ExtractionBuffer)NE2(ExtractionBuffer)PIC(1000XProteaseInhibitorCocktail)1000XDTTSolutionUserGuide

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