首页 > Epigentek > Epigentek/EpiQuik Global Histone H3 Phosphorylation (Ser10) Assay Kit (Fluorometric)/P-7003-48/48 assays

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Epigentek/EpiQuik Global Histone H3 Phosphorylation (Ser10) Assay Kit (Fluorometric)/P-7003-48/48 assays

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InputType:CellExtracts,HistoneExtracts,UnisolatedSamplesResearchArea:PhosphorylationTargetApplication:AmountQuantitationVesselFormat:96-WellPlate100%Guarantee:6monthsProductOverviewTheEpiQuik™GlobalHistoneH3Phosphorylation(Ser10)AssayKit(Fluorometric)isaconvenientsetoftoolsthatallowstheexperimentertomeasureglobalphosphohistoneH3(ser10).Thekitisready-to-useandprovidesalltheessentialcomponentsneededforspecificallymeasuringglobalhistoneH3phophorylationatser10usingavarietyofmammaliancells(human,mouse,etc.)includingfreshandfrozentissues,andculturedadherentandsUSPensioncells.Thekithasthefollowingadvantages:Quickandefficientprocedure,whichcanbefinishedwithin3hours.InnovativefluorometricassaywithouttheneedforrADIoactivity,electrophoresis,orchromatography.SpecificallycapturesphosphohistoneH3(ser10)withthedetectionlimitaslowas2ng/well.ThecontrolisconvenientlyincludedforthequantificationofphosphorylatedhistoneH3(ser10).StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughput.Simple,reliable,andconsistentassayconditions.Principle&ProcedureTheEpiQuik™GlobalHistoneH3Phosphorylation(Ser10)AssayKit(Fluorometric)isdesignedformeasuringglobalhistoneH3phosphorylationatser10.Inanassaywiththiskit,thephosphorylatedhistoneH3atser10iscapturedtothestripwellscoatedwithananti-phosphohistoneH3(ser10)antibody.ThecapturedphosphohistoneH3(ser10)canthenbedetectedwithalabeleddetectionantibody,followedbyacolordevelopmentreagent.TheratioofphosphohistoneH3(ser10)isproportionaltotheintensityofabsorbance.TheabsoluteamountofphosphohistoneH3(ser10)canbequantitatedbycomparingtothestandardcontrol. Fig.1.SchematicprocedureforusingtheEpiQuikGlobalHistoneH3Phosphorylation(Ser10)AssayKit(Fluorometric).ProductComponentsF1(10XWashBuffer)F2(AntibodyBuffer)F3(DetectionAntibody,1mg/ml)*F4(FluoroDeveloper)*F5(FluoroEnhancer)*F6(FluoroDilution)StandardControl(100µg/ml)*8-WellSampleStrips(withFrame)8-WellStandardControlStripsUserGuide*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialpriortoopeningthecap.

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