首页 > Epigentek > Epigentek/EpiNext DNA Purification HT System/P-1063-64/64 ml

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Epigentek/EpiNext DNA Purification HT System/P-1063-64/64 ml

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P-1063-64

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InputType:DNAResearchArea:NextGenSequencingTargetApplication:LibraryConstructionVesselFormat:Columns/Tubes100%Guarantee:6monthsProductOverviewTheEpiNext™ DNA PurificationHTSystem(alsoknownas"EpiNext™beads"or"MQ beads") utilizesmagneticbeadtechnologyforhighthroughputDNAorPCRampliconpurificationandDNAsizeselection.Thesystemcanalsobe usedforconcentratingDNAandissuitableforselectivelyandconsistentlycapturingDNAfragmentsorPCRampliconsthatare100bpsorlargerinsize.Thesystemhasthefollowingadvantages:Optimizedbufferchemistries: CompleteseparationofDNAorPCRamplicons.ItcanalsobeusedforDNAsizeselectionbasedontheratioofbeadstoDNAsamplevolume. Fastandstraightforward:Theentireprocedurecanbefinishedinjust30minfor96samplesandishighlyamenabletoavarietyofautomationplatforms.Nogels,filtration,centrifugation,or columns areneeded. Efficientclean-up:Removalofexcessprimers,adaptors,nucleotides,salts,enzymes,andPCRinhibitingsubstancessuchaspolysaccharides,polyphenols,lipidsanddyes.HighrecoveryofDNA:Higherthan98%recoveryofinputDNA. Manualandautomationfriendly:Scalableforuseinsingletubeor96-wellplateformats.Ultimatevalue:SuperiorperformanceforlessthanhalfthepricevsAgencourt® AMPure® XPbeads,without havingtochangeanyofyourexistingworkflowsorprotocols.BackgroundInformationObtaininghighrecoveryofpurifiedDNAorselectedDNAfragmentsiscriticalfordownstreamapplicationsthatuseDNAsamplesincludingPCR,sequencing,cloning,microarray,andDNAfragmentanalysis,regardlessoftheplatformused. Principle&ProcedureThesystemcontainsanoptimizedMQbindingbeadsolutiondevelopedbyEpigentekwhichallowsDNAorPCRampliconstobindtightlytothebeads.Excessprimers,adaptors,nucleotides,salts,enzymes,andPCRinhibitingsubstancescanberemovedbysimplywashingthebeads.OptimizationofMQbeadratiotoinputDNAallowsDNAsizeselectionbytheremovaloflargerorsmallerDNAfragmentsandrecoveryofdesiredtargetsizeDNAfragments.StartingMaterialsDNAfragmentsofvariouslengths.Inputamountcanbefrom0.1ngto1µg.Thebeadvolumesprovidedinthesystemof4mlor8mlpermita totalof96or192standardpurifications,respectively(use0.5µgofDNAin20µlofsolution);or48or96standardDNAsizeselections,respectively(use50µlofinputDNAsolution). Agencourt® AMPure®isaregisteredtrademarkofBeckmanCoulter,Inc.Fig.1. WorkflowoftheEpiNext™DNAPurificationHTSystem.Fig.2. Gelpictureafterpurificationof800ngofDNAMarker(Hyperladder50bpladder,Bioline)usingtheEpiNext™DNAPurificationHTSystemwithvaryingratiosofbeadsfrom0.9Xto2.1X.AMPureXPbeadswereusedasthecontrol.M:MarkersizeofAgilentDNAchipkit. Fig.3. PercentageofrecoveryofinputDNAfragmentsby"AMPureXP"beadsvs“EpiNext”beads:780ngofDNAmarker(Hyperladder50bpladder,Bioline)werepurifiedusingtheEpiNext™DNAPurificationHTSystemat1.8X.AgencourtAMPureXPbeads(1.8X)wereusedasthecontrol.Recoveryyieldof100bporhigherDNAfragmentswasquantifiedwithanAgilentBioanalyzer2100.ProductComponentsMQBindingBeadsUserGuide

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