首页 > Epigentek > Epigentek/ChromaFlash Chromatin Extraction Kit/P-2001-100/100 extractions

提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服核对商品价格，订单生效后再付款。

Epigentek/ChromaFlash Chromatin Extraction Kit/P-2001-100/100 extractions

价格：

登录查看

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号：

P-2001-100

品牌：

Epigentek

会员服务：

尊享会员价

贵宾专线

运费优惠

闪电退款

福利优惠

上门换新

友情提示

以上价格仅为参考，请联系客服询价。

免费咨询热线

4000-520-616

产品说明

InputType:UnisolatedSamplesResearchArea:Chromatin&TranscriptionTargetApplication:SampleIsolationVesselFormat:Columns/Tubes100%Guarantee:6monthsProductOverviewThe ChromaFlash™ChromatinExtractionKit isacompletesetofoptimizedbuffersandreagentsforisolatingchromatinorDNA-proteincomplexfrommammaliancellsortissuesinasimpleandrapidformat.Chromatinpreparedbythiskitcanbeusedinavarietyofchromatinimmunoprecipitationmethods.ItisalsotherecommendedmethodforobtainingchromatinrequiredbyEpigentek’sone-hourChIPmethodusingthe ChromaFlash™One-StepChIPKit.Theisolatedchromatincanalsobeusedinotherchromatin-relatedapplicationssuchasinvitroprotein-DNAbindingassaysandnuclearenzymeassays.Extremelyfastprocedure:theentireprocedurefromcell/tissuesampletoready-to-usechromatinislessthan60minutes. ConvenientandflexIBLe:thekitissuitableforpreparingbothnativechromatinandcross-linkedchromatinfrommonolayerorsUSPensioncells,orfromtissues. Unshearedchromatinmakesitcustomizableforvariousanalysisworkflowsthatrequireeitherintactorfragmentedchromatin,includingChIP,invitroprotein-DNAinteractionanalysis,nuclearenzymeassay,etc.BackgroundInformationChromatinimmunoprecipitaton(ChIP)offersanadvantageoustoolforstudyingprotein-DNAinteraction.WithChIP,theexperimentercandetermineifaspecificproteinbindstothespecificsequencesofageneinlivingcellsbycombiningwithPCR(ChIP-PCR),microarray(ChIP-chip),orsequencing(ChIP-Seq)techniques.Forexample,themeasurementoftheamountofmethylatedhistoneH3atlysine9(meH3-K9)associatedwithaspecificgenepromoterregionundervariousconditionscanbeachievedthroughaChIP-PCRassay,whilerecruitmentofmeH3-K9tothepromotersonagenome-widescalecanbedetectedbyChIP-chip.Inparticular,theChIPmethodwithspecificantibodiesdirectlyagainstvarioustranscriptionalfactorsiswidelydemanded. Principle&ProcedureTheChromaFlash™ChromatinExtractionKitcontainsallreagentsrequiredforcarryingoutsuccessfulchromatinextractiondirectlyfrommammaliancellsortissues.Cellmembranesofthesample,withorwithoutcross-linking,arebrokendownusingtheprovidedlysisbuffer.ChromatinorDNA-proteincomplexisthenextractedwiththeextractionbuffer.Theextractedchromatincanthenbedilutedwithchromatinbufferandstoredattheappropriatetemperature.StartingMaterials&InputAmountStartingmaterialscanincludevarioustissueorcellsamplessuchascellsfromflaskormicroplateculturedcells,freshandfrozentissues,etc.Theamountofcellsandtissuesforeachpreparationcanbe1x105 to5x106 cellsand10mgto200mg,respectively.Foroptimalpreparation,theinputamountshouldbe1to5x106 cellsor50to200mgtissues. Yieldofchromatinisapproximately4ugper106 cellsorper50mgtissues.ProductComponents10XLysisBufferExtractionBufferChromatinBufferProteaseInhibitorCocktails(1000X)UserGuide

Epigentek快速，有效地制备DNA，蛋白质和染色质对于进行下游分析至关重要。我们的样品制备套件套件几乎适用于所有工作流程，无论是为ChIP-Seq或ChIP-PCR应用分离染色质，还是从各种起始原料制备亚硫酸氢盐-Seq或MS-PCR的DNA。我们的样品制备套件使您可以直接从提取到反应，为您的实验工作流程提供无数种可能性和灵活性。我们的DNA，蛋白质和染色质制备试剂盒是无数研究机会和下游应用的起点。