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Epigentek/EpiQuik Total RNA Isolation Kit (Tissue)/P-9102-050/50 samples

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P-9102-050

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InputType:TissuesResearchArea:RNAMethylationTargetApplication:SampleIsolationVesselFormat:Columns/Tubes100%Guarantee:6monthsProductOverviewTheEpiQuik™ TotalRNAIsolationKit(Tissue) providesafast,simple,andcost-effectivemethodforisolatingtotalRNAfromtissuesamples.DetergentsandchaotropicsaltareusedtolysecellsandinactivateRNase.Thespecializedhigh-saltbufferingsystemallowsRNAspeciesbasestobindtotheglassfibermatrixofthespincolumnwhilecontaminantspassthroughthecolumn.Impuritiesareefficientlywashedaway,andthepureRNAiselutedwithREBufferwithoutphenolextractionoralcoholprecipitation.RNApurifiedwithTheTotalRNAIsolationKit(Tissue)issuitableforavarietyofroutineapplicationsincludingRT-PCR,CDNASynthesis,NorthernBlotting,Differentialdisplay,PrimerExtensionandmRNASelection.Theentireprocedurecanbecompletedwithin25-40minutes. Thekithasthefollowingfeaturesandadvantages:Fastproceduredelivershigh-qualitytotalRNAin25-40minutesReady-to-useRNAforhighperformanceinanydownstreamapplicationConsistentRNAyieldfromasmallamountofstartingmaterialPrinciple&ProcedureThiskitcontainsallthereagentsrequiredforsuccessfullyperformingRNAisolationdirectlyfromtissue.Afterlysis,binding,andwash,RNAiseasilyrecoveredinquantitiesofupto30µgusingspeciallydesignedcolumns.TotalRNAisthenreadytobeusedforavarietyofdownstreamapplications.StartingMaterialsTheamountofstartingmaterialscanbeupto30mgoftissue,orupto25mgofparaffin-embeddedtissue.Atotalof50standardextractionscanbeperformedwiththiskit. YieldofthetotalRNAcanbeupto30µg.Theyieldmayvarydependingonthesampletype. ProductComponentsRR(LysisBuffer)W1(WashBuffer-1)W2(WashBuffer-2)*RE(ElutionBuffer)T-ColumnCollectionTubes*Add30mlethanol(96~100%)totheW2(WashBuffer-2),andshakebeforeuse.

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