首页 > Epigentek > Epigentek/EpiQuik Total RNA Isolation Kit (Plant)/P-9104-050/50 samples

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Epigentek/EpiQuik Total RNA Isolation Kit (Plant)/P-9104-050/50 samples

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P-9104-050

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InputType:PlantCellsorTissueResearchArea:RNAMethylationTargetApplication:SampleIsolationVesselFormat:Columns/Tubes100%Guarantee:6monthsProductOverviewTheEpiQuik™ TotalRNAIsolationKit(Plant) providesafast,simple,andcost-effectivemethodforisolatingtotalRNAfromplanttissuesamples.DetergentsandchaotropicsaltareusedtolysecellsandinactivateRNase.Thespecializedhigh-saltbufferingsystemallowsRNAspecieslongerthan100basestobindtotheglassfibermatrixofthespincolumn.TheTotalRNAIsolationKitissuitableforavarietyofroutineapplicationsincludingRT-PCR,CDNASynthesis,NorthernBlotting,Differentialdisplay,PrimerExtensionandmRNAselection. Theentireprocedurecanbecompletedwithin60minutes. Thekithasthefollowingfeaturesandadvantages:Fastproceduredelivershigh-qualitytotalRNAin60minutesReady-to-useRNAforhighperformanceinanydownstreamapplicationConsistentRNAyieldfromasmallamountofstartingmaterialPrinciple&ProcedureThiskitcontainsallthereagentsrequiredforsuccessfullyperformingRNAisolationdirectlyfromplanttissues.Afterlysis,binding,andwash,RNAiseasilyrecoveredinquantitiesofupto30µgusingspeciallydesignedcolumns.TotalRNAisthenreadytobeusedforavarietyofdownstreamapplications. StartingMaterialsTheamountofstartingmaterialscanbe100mgoffreshplanttissueor25mgofdryplanttissue.Atotalof50standardextractionscanbeperformedwiththiskit. YieldofthetotalRNAcanbeupto30µg.Theyieldmayvarydependingonthesampletype. ProductComponentsRP(Pre-lysisBuffer)W1(WashBuffer-1)W2(WashBuffer-2)*RE(ElutionBuffer)P-ColumnCollectionTubes*Add30mlethanol(96~100%)totheW2(WashBuffer-2),andshakebeforeuse.

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