首页 > Epigentek > Epigentek/ChromaFlash Plant Chromatin Extraction Kit/P-2022-050/50 extractions

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Epigentek/ChromaFlash Plant Chromatin Extraction Kit/P-2022-050/50 extractions

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P-2022-050

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InputType:UnisolatedSamplesResearchArea:Chromatin&TranscriptionTargetApplication:SampleIsolationVesselFormat:Columns/Tubes100%Guarantee:6monthsProductOverviewTheChromaFlash™PlantChromatinExtractionKitisacompletesetofoptimizedbuffersandreagentsforisolatingchromatinorDNA-proteincomplexesfromplantsinasimpleandrapidformat.Chromatinpreparedbythiskitcanbeusedinavarietyofchromatinimmunoprecipitationmethods.ItisalsotherecommendedmethodforobtainingchromatinrequiredbyEpigentek’sone-hourChIPmethodusingtheChromaFlash™One-StepChIPKitortheChromaFlash™One-StepMagneticChIPKit.Theisolatedchromatincanalsobeusedinotherchromatin-relatedapplicationssuchasinvitroprotein-DNAbindingassays,ornuclearenzymeassays.Extremelyfastprocedure:theentireprocedurefromplantsamplestoready-to-usechromatinislessthan2hours.ConvenientandflexIBLe:thekitissuitableforpreparingbothnativechromatinandcross-linkedchromatinfrommonolayerorsUSPensioncells,orfromtissues. Unshearedchromatinmakesitcustomizableforvariousanalysisworkflowsthatrequireeitherintactorfragmentedchromatin,includingChIP,invitroprotein-DNAinteractionanalysis,nuclearenzymeassays,etc.BackgroundInformationChromatinimmunoprecipitation(ChIP)offersanadvantageoustoolforstudyingprotein-DNAinteraction.WithChIP,theexperimentercandetermineifaspecificproteinbindstothespecificsequencesofageneinlivingcellsbycombiningitwithPCR(ChIP-PCR),microarray(ChIP-chip),orsequencing(ChIP-Seq)techniques.Forexample,themeasurementoftheamountofmethylatedhistoneH3atlysine9(meH3-K9)associatedwithaspecificgenepromoterregionundervariousconditionscanbeachievedthroughaChIP-PCRassay,whilerecruitmentofmeH3-K9tothepromotersonagenome-widescalecanbedetectedbyChIP-chip.Inparticular,aChIPmethodwithspecificantibodiesdirectlyagainstvarioustranscriptionalfactorsiswidelyindemand.Principle&ProcedureTheChromaFlash™ PlantChromatinExtractionKitcontainsallthereagentsrequiredforcarryingoutasuccessfulchromatinextractiondirectlyfromplants.Cellmembranesofthesample,withorwithoutcross-linking,arebrokendownusingtheprovidedlysisbuffer.ChromatinorDNA-proteincomplexisthenextractedwiththeextractionbuffer.Theextractedchromatincanthenbedilutedwithchromatinbufferandstoredattheappropriatetemperature.Fig.1. SchematicprocedureoftheChromaFlash™ PlantChromatinExtractionKit.Fig.2.ChIPanalysisoftri-methylH3-K27enrichedinAGAMOUSgenewithchromatinextractpreparedfrom2-week-oldicu2-1/icu2-1seedlingsusingtheChromaFlash™ PlantChromatinExtractionKit.ProductComponents5XLysisBufferExtractionBufferAExtractionBufferBExtractionBufferC ChromatinBufferProteaseInhibitorCocktails(1000X)*UserGuide*Spinthesolutiondowntothebottompriortouse.

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