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Epigentek/EpiNext DNA Size Selection Kit/P-1059-48/48 reactions

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P-1059-48

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InputType:DNAResearchArea:DNAMethylation,NextGenSequencingTargetApplication:LibraryConstructionVesselFormat:96-WellPlate100%Guarantee:6monthsProductOverviewTheEpiNext™ DNASizeSelectionKitisacompletesetofoptimizedreagentsforquickremovalofDNAfragmentsof<150bpsforlibrarypreparationinnextgenerationsequencingapplications.ThekitutilizesmagneticbeadtechnologyandissuitableforIllumina, LifeTechnologies(SOLiD), IonTorrent,andRoche/454platforms.ItcanalsobeusedforremovingundesiredlargerDNAfragmentsbyoptimizingthebeadtoDNAvolumeratioaccordingly.Theindicatednumberofreactionscanbeperformedforastandard50µlsolutioninputDNAsample.Thekithasthefollowingadvantages:Optimizedfragmentselectionchemistries:CompleteseparationofDNAfragmentsaccordingtosize.SuitableforIllumina, IonTorrent,SOLiDand Roche/454platforms.Fastandstraightforward: Sizeselectionprocedurecanbefinishedwithin30min.Nogels,columnsorcentrifugationareneeded.Efficientremovalofprimer-dimers:Noneedforfurtherclean-upsteps.HighrecoveryoftargetedDNAfragments:Higherthan85%recoveryofinputDNAfragments. Manualandautomationfriendly:Scalableforuseinsingletubeor96-wellplateformat.BackgroundInformationDNAsizeselectionisnecessaryafterDNAshearingaspartofthelibraryconstructionprocessfornextgenerationsequencing,regardlessoftheplatformused.ObtaininghighrecoveryofselectedDNAfragmentsiscriticalforthereductionofsequencingbias. Principle&ProcedureThiskitincludesallreagentsrequiredateachstepoftheworkflowforDNAsizeselection.ThesizeofDNAfragmentsboundtotheincludedMQBindingBeadsisbasedontheratioofMQbeadstotheDNAsamplesolution.OptimizationofMQbeadratiotoinputDNA,allowsfortheremovaloflargerorsmallerDNAfragmentsandrecoveryofdesiredtargetsizeDNAfragments.StartingMaterialsDNAfragmentsofvariouslengths.Inputamountcanbefrom0.1ngto1µg. Fig.1. WorkflowoftheEpiNext™ DNASizeSelectionKit.Fig.2. SizeselectionofDNAfragments:HumanPlacentaDNAwasshearedto100-700bpsinlengthwithpeaksizeofabout300bps(A).Atargetpeaksizeof500bps(B)wasselectedusingtheEpiNext™ DNASizeSelectionKit.ProductComponentsMQBindingBeads*ElutionSolution*96-wellplateUserGuide*Spinthesolutiondowntothebottompriortouse.

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