首页 > Epigentek > Epigentek/EpiNext NGS Barcode (Index) Set-12/P-1060-144/144 reactions

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Epigentek/EpiNext NGS Barcode (Index) Set-12/P-1060-144/144 reactions

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P-1060-144

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4000-520-616

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InputType:DNAResearchArea:NextGenSequencingTargetApplication:LibraryConstructionVesselFormat:Columns/Tubes100%Guarantee:6monthsProductOverviewTheEpiNext™NGSBarcode(Index)Set-12isdesignedtoconstructmultiplexDNA/RNAlibrariesusedfornext-generationsequencingwiththeIlluminaplatformincludingGAIIx,HiSeq,MiSeq,andNextSeq. Thisproductincludes12DNAbarcodes,eachofwhichcontainanindexandflowcellbindingsequenceandcanbeattachedtothesampleinsertduringlibrarypreparation.MultiplexingseveralsamplesinasingleflowcellcanbeachievedbypoolingwiththeEpiNext™NGSBarcodes.Poolingbarcodedsamplesintoasingleflowcellsignificantlyreduceshands-ontimeandprovidesrobustdataqualityinNGS.TheEpiNext™NGSBarcode(Index)Set-12hasthefollowingfeatures:ThebarcodescontainedintheEpiNext™NGSBarcode(Index)Set-12canbeusedwithsingle,paired-endandmultiplexreadsandarecompatIBLewithvariouslibrarypreparationworkflowsusedforIlluminaNGS,includingDNA-seq,RNA-seq,ChIP-seq,MethylC-seq,MeDIP-seq,hMeDIP-seq,DNAbisulfite-seq,oxBS-seqandRNAbisulfite-seq. ThebarcodesarealsooptimizedforusewithEpigentek’sEpiNext™NGSlibrarypreparationkitseriesincludingEpiNext™DNALibraryPreparationKit(Illumina),EpiNext™High-SensitivityDNALibraryPreparationKit (Illumina),EpiNext™Post-BisulfiteDNALibraryPreparationKit (Illumina),EpiNext™Bisulfite-SeqHigh-SensitivityKit (Illumina),EpiNext™ChIP-SeqHigh-SensitivityKit (Illumina),EpiNext™RNABisulfite-SeqKitandEpiNext™MeDIP-SeqKit.Highthroughputandcostreduction:Upto144multiplexedsamplescanbeprocessed,whichallowsmultiplexingof2-12fragmentlibrarysamplesonasinglesequencingflowcellresultinginconsiderablereductionofper-samplecost.Theindicesaredesignedtobeerrorresistantandallowforproperdifferentiationbetweensamples.MinimizesPCRamplification-basedbiasandpreventspoorreadsfromsinglebaseerrorsintroducedduringPCR.Fig.1. WorkflowoftheDNAlibrarypreparationwiththeEpiNext™NGSBarcode(Index)Set-12. ProductComponentsEpiNext™Barcodes1-12(10µM)*†EpiNext™UniversalPrimer(10µM)*UserGuide*Spinthesolutiondowntothebottompriortouse.† TheEpiNext™NGSBarcodeSet-12contains12DNAbarcodeswithsufficientvolumefor12reactionseach,foratotalof144reactions.Theshelflifeofeachreagentis6monthswhenstoredat-20°C.

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