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Epigentek/EpiNext Hi-Fi cDNA Synthesis Kit/P-9004-20/20 samples

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P-9004-20

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InputType:RNAResearchArea:RNAMethylationTargetApplication:LibraryConstruction,PCRAmplification,SampleModificationVesselFormat:Columns/Tubes100%Guarantee:6monthsProductOverviewThe EpiNext™Hi-FiCDNASynthesisKit isacompletesetofoptimizedreagents tosynthesizeaDNAcopyspecificallyusingbisulfite-convertedRNAandenrichedRNAfragments. OptimizedtosynthesizeDNAcopiesfrombisulfite-convertedRNAAlsosuitableforcDNAsynthesisfromtotalRNASynthesizedDNAisfullycompatIBLewithPCRandcDNAlibraryconstructionIncludedRTenzymehaslowinstrinsicRNaseactivitytodeliverextremelyhighreversetranscriptionaccuracyandpromotesfull-lengthcDNAsynthesisDescriptionEffectiveandefficientpreparationofcDNAfromRNAcanhelpensurereliabledatainvariousdownstreamapplicationsincludingreal-timeqPCRandRNA/cDNA-sequencing.TheEpiNext™ Hi-FicDNASynthesisKithasbeenoptimizedandvalidatedforcDNAsynthesisfromvariousRNAsamples,particularlyfrombisulfite-convertedRNAandenrichedRNAfragments.ThekitcontainsallnecessarycomponentsincludingahighlysensitiveandThermostablerecombinantreversetranscriptasewhichprovidesenhancedcDNAsynthesisefficiency,highfidelity,andawiderangeofactivityforvaryingamountsofRNAtemplates(0.1ngto2 µg).Due toitshighthermalstABIlity,lowRNaseactivityandresistancetoinhibitionbyrRNAandtRNA,therecombinantRTincludedinthekitcanbeusedforsynthesisoflongcDNAproductsattemperaturesfrom42°C-60°CusingtotalRNA,andGC-richtemplatessuchasbisulfiteconvertedRNA.ArecombinantRNaseinhibitorisalsoincludedinthekittopreventthedegradationoftargetRNAduetoribonucleasecontamination.StartingMaterialsRNAisolatedfromvarioustissueorcellsamplescanbeusedasstartingmaterial.TheamountofRNAforeachreactioncanbe0.1ngto2µg.Foranoptimalreaction,theinputRNAamountshouldbe200ngto500ng. Fig.1. TheEpiNextHiFicDNASynthesisKitmechanismusesbothA)oligo-dTandB)randomhexamers. ProductComponents5XRTreactionbuffer10mMdNTPmix*0.1MDTT*RNaseinhibitor*Randomprimer(50µM)*RTenzymemix*UserGuide*Spinthesolutiondowntothebottompriortouse.

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