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Epigentek/Methylamp Coupled DNA Isolation & Modification Kit/P-1002-40/40 samples

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P-1002-40

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InputType:DNAResearchArea:DNAMethylationTargetApplication:SampleIsolation,SampleModificationVesselFormat:Columns/Tubes100%Guarantee:6monthsProductOverviewTheMethylamp™CoupledDNAModificationKitisacompletesetofessentialcomponentswhichenablestheexperimentertoisolateandmodifyDNAefficiently.Theentireprocedurecanbecompletedwithin2hoursand20minutesandproducesfarsuperiorresultsthananycompetitorkits.TheMethylamp™CoupledDNAModificationKitissuitableforMS-PCR,realtimeMS-PCR,methylationsequencing,andpyrosequencing,aswellasmethylationmicroarray.Thekithasthefollowingadvantages:Thefastestprocedureavailable,whichcanbefinishedwithin2hoursand20minuteswithconsistentreactionconditions.HighefficiencyofDNAisolationfrommultipleresourcesofBIOLOGicalmaterialcontainingtinyamountsofDNA(aslowas0.1ng),especiallyfromserum,plasmaandbodyfluids.Completelyconvertsunmethylatedcytosineintouracil:modifiedDNA>99.98%.ThelowestdegradationofDNAinthemodificationprocess:morethan90%ofDNAlosscanbeprevented.ThelowestrequirementofstartingDNAformodification:only50pgor20cells.Simple,reliable,andconsistentmodificationconditions.Principle&ProcedureTheMethylamp™CoupledDNAIsolationandModificationKitcontainsallreagentsrequiredforDNAisolationandbisulfiteconversion.DNAisisolatedandchemicallydenaturedtoallowbisulfitereagenttoreactspecificallywithsingle-strandedDNA,therebydeaminatingcytosineandcreatingauracilresidue.TheuniqueDNAprotectionreagentscontainedinthemodificationbuffercanpreventthechemicalandThermophilicdegradationofDNAinthebisulfitetreatment.Thenon-toxicmodifiedDNAcapturebufferenablesDNA totightlybindtothecolumnfilter,thusDNAcleaningcanbecarriedoutonthecolumntoeffectivelyremoveresidualsodiumbisulfiteandsalts.ModifiedDNAthencanbeelutedandstablystoredat-20°Cforupto2months. Fig.1.SchematicprocedureforusingtheMethylampCoupledDNAIsolation&ModificationKit(Colorimetric).ProductComponentsMR1(DNADigestionSolution)MR2(DNADigestionPowder)MR3(DNAIsolationBuffer)MR4(DNAIsolationEnhancer)MR5(DNADenatureSolution)MR6(DNAModifierPowder)MR7(DNAModifierSolution)MR8(DNABindingBuffer)MR9(DNAWashingBuffer)MR10(DNAElutionbuffer)1XTEBufferF-SpinColumnF-CollectionTubeUserGuide

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