首页 > Epigentek > Epigentek/Methylamp 96 DNA Modification Kit/P-1008-2/192 samples

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Epigentek/Methylamp 96 DNA Modification Kit/P-1008-2/192 samples

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P-1008-2

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InputType:DNAResearchArea:DNAMethylationTargetApplication:SampleModificationVesselFormat:96-WellPlate100%Guarantee:6monthsProductOverviewTheMethylamp™96DNAModificationKitisacompletesetofessentialcomponentswhichenablestheexperimentertomodifyDNAefficiently.Theentireprocedurecanbecompletedwithin2hoursand30minutesandproducesfarsuperiorresultsthananycompetitorkits.TheMethylamp™96DNAModificationKitissuitableforMS-PCR,realtimeMS-PCR,methylationsequencing,andpyrosequencing,aswellasmethylationmicroarray.Thekithasthefollowingadvantages:Thefastestprocedureavailable,whichcanbefinishedwithin2hoursand30min.Highthroughputisachievablealongwitha96-wellfilterformat.Completelyconvertsunmethylatedcytosineintouracil:modifiedDNA>99.98%.ThelowestdegradationofDNAinthemodificationprocess:morethan90%ofDNAlosscanbeprevented.ThelowestrequirementofstartingDNAformodification:only1ng.Simple,reliable,andconsistentmodificationconditions.Principle&ProcedureTheMethylamp™96DNAModificationKitcontainsallreagentsrequiredforbisulfiteconversiononaDNAsample.DNAisdenaturedbyheating,whichallowsDNAdenaturationandbisulfitemodificationtobecarriedoutsimultaneously.Inthemodificationprocess,bisulfitereagentreactsspecificallywithsingle-strandedDNA,therebydeaminatingcytosineandcreatingauracilresidue.TheuniqueDNAprotectionreagentscontainedinthemodificationbuffercanpreventthechemicalandThermophilicdegradationofDNAinthebisulfitetreatment.Thenon-toxicmodifiedDNAcapturebufferenablesDNAtotightlybindtothecolumnfilter,thusDNAcleaningcanbecarriedoutonthecolumntoeffectivelyremoveresidualsodiumbisulfiteandsalts.ModifiedDNAthencanbeelutedandstablystoredat-20°Cforupto2months. Fig.1.SchematicprocedureforusingtheMethylamp96DNAModificationKitProductComponentsCF1(DNAModificationPowder)CF2(DNAModification)CF3(BalanceBuffer)CF4(DNACarrier)CF5(ModifiedDNACapture)CF6(ModifiedDNAElution)96-WellPCRPlate96-WellFilterPlateReceiverPlateOpticalAdhesiveCoverUserGuide

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