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InputType:NuclearExtracts,PurifiedEnzymeResearchArea:HistoneAcetylationTargetApplication:ActivityMeasurementVesselFormat:96-WellPlate100%Guarantee:6monthsProductOverviewWerecommendusinganewerversionofthisproduct:EpigenaseHDACActivity/InhibitionDirectAssayKit(Fluorometric).TheEpiQuik™HDACActivity/InhibitionAssayKit(Fluorometric)isaready-to-usesetofessentialcomponentsneededtomeasureHDACactivity/inhibitionsafelyandquickly.ItissuitableformeasuringHDACactivity/inhibitionfromabroadrangeofspeciesincludingmammaliancells/tissues,plants,andbacteria. WHYCHOOSETHEEPIQUIK™HDACACTIVITY/INHIBITIONASSAYKIT(FLUOROMETRIC),Fastprocedure,whichcanbefinishedwithin3hours.InnovativefluorometricassaywithouttheuseofrADIoactivity,extraction,andchromatography.DirectmeasurementofHDACactivityandinhibitionwithnouseoflysylendopeptidase,therebyavoidingthefalseinhibitoryeffectonHDACsandallowingmoreaccuratemeasurement.StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.Simple,reliable,andconsistentassayconditions.Fig.1. HDACassaystandardcurvewasgeneratedasdescribedintheprotocolof EpiQuik™HDACActivity/InhibitionAssayKit(P-4001). ProductComponentsH1(10XWashBuffer)H2(HDACAssayBuffer)H3(BiotinylatedHDACSubstrate)*H4(HDACInhibitor,0.5mM)*H5(HDACStandard,20µg/ml)*H6(CaptureAntibody,1000µg/ml)*H7(DetectionAntibody,200µg/ml)*FluoroDeveloper8-WellAssayStrip(withFrame)UserGuide*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialafterthawingpriortoopeningthecap.
Epigentek快速,有效地制备DNA,蛋白质和染色质对于进行下游分析至关重要。我们的样品制备套件套件几乎适用于所有工作流程,无论是为ChIP-Seq或ChIP-PCR应用分离染色质,还是从各种起始原料制备亚硫酸氢盐-Seq或MS-PCR的DNA。我们的样品制备套件使您可以直接从提取到反应,为您的实验工作流程提供无数种可能性和灵活性。我们的DNA,蛋白质和染色质制备试剂盒是无数研究机会和下游应用的起点。
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