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Cell Biologics/Porcine Primary Skeletla Muscle Microvascular Endothelial Cells/P-6220/

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P-6220

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4000-520-616

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PorcinePrimarySkeletlaMuscleMicrovascularEndothelialCellsCatalogNo. P-6220SuggestedMedium: EndothelialCellMedium/wKit–500mlCatalogNo. 1168CustomOrder(1-3weeks)ProductDescriptionPorcinePrimarySkeletalMuscleMicrovascularEndothelialCellsfromCellBIOLOGicsareisolatedfromskeletalmuscletissueofporcine.PorcinePrimarySkeletalMuscleMicrovascularEndothelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106cellspermlandaredeliveredfrozen.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticpre-coatedwithsecondaryantibody.ProductTestingPorcinePrimarySkeletalMuscleMicrovascularEndothelialCellsaretestedforuptakeofDil-Ac-LDL(CatalogNo.L-35353,Invitrogen),afunctionalMarkerforendothelialcells.PorcinePrimarySkeletalMuscleMicrovascularEndothelialCellsarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.LaboratoryApplicationsPorcinePrimarySkeletalMuscleMicrovascularEndothelialCellscanbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,ortransendothelialresistance(TER).StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.StorageofCellBiologics’ProductsCellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Nevercanprimarycellsbekeptat-20°C.AuthorizedUsesofCellBiologics’ProductsPorcinePrimarySkeletalMuscleMicrovascularEndothelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.DisclaimerInvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.WarrantyandLiABIlityCellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.

Cell Biologics细胞生物制剂公司总部位于美国芝加哥，是全球独家提供Jackson Lab基因敲除小鼠原代细胞的专业公司，产品已获美国农业部SPF认证，并经中国进出口商品检验检疫局检验准许进口自成立以来，Cell Biologics已开发出13个品系1,000多种可直接使用的JacksonLab基因敲除小鼠、正常野生型小鼠、大鼠原代细胞，必将填补细胞生物学界研究的空白。另外，Cell Biologics拥有AAALAC认证的动物实验室，提供JacksonLab与国外其他著名实验室所有允许商用的基因敲除小鼠原代细胞分离服务