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Cell Biologics/Porcine Primary Prostate Epithelial Cells/P-6038/ Frozen

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P-6038

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Porcine Primary Prostate Epithelial Cells Catalog No. P-6038 Suggested Medium: Epithelial Cell Medium /w Kit – 500 ml Catalog No. H6621 Product Description Porcine Primary Prostate Epithelial Cells from Cell Biologics are isolated from prostate tissue of porcine. Porcine Primary Prostate Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody. Product Testing Porcine Primary Prostate Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin or ZO-1. Porcine Primary Prostate Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended. Laboratory Applications Porcine Primary Prostate Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. Storage of Cell Biologics’ Products Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C. Authorized Uses of Cell Biologics’ Products Porcine Primary Prostate Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order. Disclaimer Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. Warranty and Liability Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

Cell Biologics细胞生物制剂公司总部位于美国芝加哥，是全球独家提供Jackson Lab基因敲除小鼠原代细胞的专业公司，产品已获美国农业部SPF认证，并经中国进出口商品检验检疫局检验准许进口自成立以来，Cell Biologics已开发出13个品系1,000多种可直接使用的JacksonLab基因敲除小鼠、正常野生型小鼠、大鼠原代细胞，必将填补细胞生物学界研究的空白。另外，Cell Biologics拥有AAALAC认证的动物实验室，提供JacksonLab与国外其他著名实验室所有允许商用的基因敲除小鼠原代细胞分离服务