Oligo Synthesis : CEPsPrices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.CatalogueDescriptionProtocolsReferencesApplications & BenefitsN4-Ac-N4-Et-dC-CE PhosphoramiditeN4-Ac-N4-Et-dC-CE PhosphoramiditeGlen ResearchCatalogue No.DescriptionPack SizePriceQtyChange to:€10-1513-02N4-Ac-N4-Et-dC-CE Phosphoramidite0.25g£540.00£513.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order10-1513-90N4-Ac-N4-Et-dC-CE Phosphoramidite100umole£180.00£171.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order10-1513-95N4-Ac-N4-Et-dC-CE Phosphoramidite50umole£100.00£95.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to OrderRelated productsPlease find below the related productsView2-Amino-dA-CE Phosphoramidite View5-Me-dC-CE Phosphoramidite View5-Me-dC-CPG ViewN4-Ac-N4-Et-dC-CE Phosphoramidite ViewN4-Et-dC-CE Phosphoramidite ViewN6-Ac-N6-Me-dA-CE Phosphoramidite ViewN6-Me-dA-CE Phosphoramidite N4-Ac-N4-Et-dC-CE PhosphoramiditeN4-Ac-N4-Et-dC-CE PhosphoramiditeGlen ResearchN4-Ac-N4-Et-dC-CE PhosphoramiditeCatalog Number: 10-1513-xxDescription: N4-Ac-N4-Et-dC-CE Phosphoramidite5"-Dimethoxytrityl-N4-acetyl-N4-ethyl-2"-deoxyCytidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramiditeFormula: C43H54N5O8PM.W.: 799.89F.W.: 317.24Diluent: Anhydrous AcetonitrileCoupling: No changes needed from standard method recommended by synthesizer manufacturer.Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.Storage: Freezer storage, -10 to -30°C, dryStability in Solution: 2-3 daysBASES AFFECTING DUPLEX STABILITYC-5 methyl pyrimidine nucleosides are known to stabilize duplexes relative to the non-methylated bases. Therefore, enhanced binding can be achieved using 5-methyl-dC in place of dC, duplex melting temperature being increased by 1.3°. Improved stacking in this case is believed to be brought about by elimination of water molecules from the duplex. 2,6-Diaminopurine 2"-deoxyriboside (2-amino-dA) forms an additional hydrogen bond with Thymidine, thereby leading to duplex stabilization with a melting temperature increase of 3°. Our 2-amino-dA monomer exhibits fast and effective deprotection in ammonium hydroxide and it is stabilized to depurination during synthesis. Sequences with high GC content may contain mismatches and still hybridize because of the high stability of the G-C base pair. The N4-ethyl analogue of dC (N4-Et-dC) hybridizes specifically to natural dG but the stability of the base pair is reduced to about the level of an AT base pair.AP-dC (G-clamp) enhances oligo hybridization since the AP-C....G base pair contains 4 hydrogen bonds, which makes the interaction much stronger than the regular C....G base pair with its 3 hydrogen bonds.Sequences with high GC content may contain mismatches and still hybridize because of the high stability of the G-C base pair. The N4-ethyl analogue of dC (N4-Et-dC) hybridizes specifically to natural dG but the stability of the base pair is reduced to about the level of an AT base pair.Coupling N6-Me-dA (10-1003) and N4-Et-dC (10-1068) with 1H-tetrazole leads to a trace of branching at the secondary amine positions, while DCI leads to around 15% branching. In collaboration with Berry and Associates, the acetyl protected monomers were prepared. Acetyl protection was chosen since it would block branching reactions. Oligonucleotides synthesized using these monomers proved to be compatible with all popular deprotection strategies from UltraMild to UltraFast. When the acetyl protected monomers were compared with the unprotected monomers using DCI as activator, branching was reduced from 15% to zero.If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200N4-Ac-N4-Et-dC-CE PhosphoramiditeN4-Ac-N4-Et-dC-CE PhosphoramiditeGlen ResearchMaterial Safety Data SheetGlen Report 23.1: New Products Prevent Branching at Secondary Amines using DCI ActivatorIf you cannot find the answer to your problem below then please contact us or telephone 01954 210 200N4-Ac-N4-Et-dC-CE PhosphoramiditeN4-Ac-N4-Et-dC-CE PhosphoramiditeGlen ResearchH K Nguyen, P Auffray, U Asseline, D Dupret and N T Thuong, Nucleic Acids Res., 1997, 25, 3059-65If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200N4-Ac-N4-Et-dC-CE PhosphoramiditeN4-Ac-N4-Et-dC-CE PhosphoramiditeGlen ResearchDILUTION/COUPLING DATAThe table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.ABI 392/394Cat.No.PackSizeGrams/Pack0.1M Dil.(mL)LV40LV20040nm0.2µm1µm10µmApproximate Number of Additions10-1513-90100µmoles.08grams120127.55.454110-1513-020.25grams.25grams3.139154.634.1324.8218.24.5510-1513-9550µmoles.04grams.53.3321.25.91.67.17ExpediteCat.No.PackSizeGrams/PackDilution(mL)Molarity50nm0.2µm1µm15µmApproximate Number of Additions10-1513-90100µmoles.08grams1.5.0723.614.7510.731.4810-1513-020.25grams.25grams4.66.0786.854.2539.455.4310-1513-9550µmoles.04grams.75.078.65.383.91.54BeckmanCat.No.PackSizeGrams/PackDilution(mL)Molarity30nm200nm1000nmApproximate Number of Additions10-1513-90100µmoles.08grams1.5.0725.215.7511.4510-1513-020.25grams.25grams4.66.0788.455.2540.1810-1513-9550µmoles.04grams.75.0710.26.384.64If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Athens Research & Technology—供应多款蛋白、酶制品

Athens Research & Technology是成立于2010年的新兴生物高科技制品公司，该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房，并且通过ISO 9001：2008认证。Athens公司致力于纯化分离高纯度，高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶，蛋白酶抑制剂，中性粒细胞酶，载脂蛋白，脂蛋白，血小板蛋白，转铁蛋白，免疫球蛋白等等。公司产品适用于炎症，冠状动脉疾病，自身免疫性疾病，癌症，阿尔茨海默氏病等众多研究领域，已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液（包括干细胞）等产品研发。除了人类蛋白质，我们还从动物血清和组织中分离多种蛋白质及酶类。此外，Athens Research and Technology还提供特殊试剂/蛋白定制服务，以满足研究人员的不同需求。