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Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-AR42-1-05
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Transfection - EZ Biosystems: Cell LinesAvalanche®-Cell Type / Cell Line-specific Transfection Reagent series:Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.For primary cells click HERETo learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERECatalogueDescriptionAR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)EZ BiosystemsCatalogue No.DescriptionPack SizePriceQtyChange to:€EZT-AR42-1-05AR42J Cell Avalanche™ Transfection Reagent0.5ml£399.00QuantityAdd to OrderEZT-AR42-1-15AR42J Cell Avalanche™ Transfection Reagent1.5ml£699.00QuantityAdd to OrderRelated productsPlease find below the related productsViewAvalanche-in vivo Transfection Reagent ViewAvalanche-Omni Transfection Reagent AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell)EZ BiosystemsCell Line Information: Designations: AR42J Organism: Rattus norvegicus (rat) Strain: Wistar Tissue: pancreas; exocrine; tumor Morphology: epithelial Growth properties: adherent Tumorigenic: yes, in athymic mice Products: amylase and other exocrine enzymes Receptors expressed: insulin; glucocorticoid Depositors: N.W. Jessop The cells grow slowly, in clusters. They tend to pile up and appear refractile. Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum.The Transfection Reagent:AR42J Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on AR42J Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for AR42J Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of AR42J Cell Avalanche™ Transfection Reagent for transfection on AR42J Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?Features:Specifically optimized to deliver nucleic acids into AR42J CellsHighest efficiency to ensure experimental success Extremely gentle to cells0.5 ml is able to transfect about 1000 wells of 24-well plateDeliver single or multiple plasmidsCompatible with serum Suitable for Reverse Transfection Compatible with transfection in any plate formatsData:FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on AR42J cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for AR42J cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this AR42J Cell Avalanche Transfection Reagent.For Other Cells:AR42J Cell Avalanche™ Transfection Reagent (rat pancreas tumor cell) can also be used on the following cells with high transfection efficiencies.Capan-1 Cell PANC-1 Cell BxPC-3 Cell AsPC-1 Cell 293 Cell 293T/17 Cell SK-OV-3 Cell U-2 OS cell Saos-2 Cell SK-N-SH CellRecommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.Research Use Only. Not for use in diagnostic or therapeutic procedures.If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Athens Research & Technology—供应多款蛋白、酶制品

Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。