Oligo Synthesis : CEPsPrices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen"sproductsarehazardousandmay be subject to additional shipping charges. Full product information is available onGlen Research"s website.CatalogueDescriptionProtocolsNotesApplications & BenefitsThiol-Modifier C6 S-SThiol-Modifier C6 S-SGlen ResearchCatalogue No.DescriptionPack SizePriceQtyChange to:€10-1936-02Thiol-Modifier C6 S-S0.25grams£200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to Order10-1936-90Thiol-Modifier C6 S-S100µmoles£80.00£76.00Offer until : 31-Mar-2021Offer Code : GLEN55% off all Glen productsView OfferQuantityAdd to OrderRelated productsPlease find below the related productsView5"-Amino-Modifier 5 View5"-Amino-Modifier C12 View5"-Amino-Modifier C3-TFA View5"-Amino-Modifier C6 View5"-Amino-Modifier C6-TFA View5"-Carboxy-Modifier C10 View5"-DMS(O)MT-Amino-Modifier C6 View5"-Thiol-Modifier C6 ViewDithiol Phosphoramidite (DTPA) ViewPC Amino-Modifier Phosphoramidite Thiol-Modifier C6 S-SThiol-Modifier C6 S-SGlen Research Thiol-Modifier C6 S-SCatalog Number: 10-1936-xxDescription: Thiol-Modifier C6 S-S1-O-Dimethoxytrityl-hexyl-disulfide,1"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramiditeFormula: C42H61N2O5S2PM.W.: 769.05F.W.: Reduced FW - 196.2 Unreduced FW - 328.4Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, seehttp://www.glenres.com/Technical/TB_ABITransfer.pdf.Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation.Deprotection: After normal deprotection, the disulfide can be cleaved at room temperature in 30 minutes with 100 mM DTT pH 8.3 - 8.5 in the buffer of your choice.Technical BulletinStorage: Freezer storage, -10 to -30°C, dryStability in Solution: 2-3 daysTERMINUS MODIFIERSGlen Research 5’-Modifiers are designed for use in DNA synthesizers to functionalize the 5’-terminus of the target oligonucleotide. The 5’-Amino-Modifiers are available with a variety of chain lengths to fit exactly the desired application.The DMS(O)MT-protected amino group is easier to deprotect compared to the MMT-protected one. The sulfoxy derivative survives conditions of oligonucleotide synthesis and can either be cleaved with standard deblock solution, or left intact for HPLC purification. At the same time, the DMS(O)MT group is fully compatible with cartridge purification. When detritylation on a cartridge is carried out, the DMS(O)MT , which is more stable than MMT , does not reattach itself to an amine. We now offer 5’-DMS(O)MT-Amino-Modifier C6 utilizing this new trityl based protecting group.5’-Amino-Modifier TEG, a hydrophilic triethylene glycol ethylamine derivative, is 12 atoms in length and fully soluble in aqueous media.The disulfide thiol modifier may be used for introducing 3’- or 5’-thiol linkages. Dithiol Phosphoramidite (DTPA) is a disulfide-containing modifier designed to functionalize synthetic DNA or RNA with multiple thiol groups and can be incorporated at any position of the oligonucleotide. Each DTPA addition leads to two thiol groups. This modifier was designed for optimal tethering of oligonucleotides to a gold surface but it can also be used for multiple reactions with maleimides and other thiol-specific derivatives. 5’-Carboxy-Modifier C10 is a unique linker designed to be added at the terminus of an oligonucleotide synthesis. It generates an activated carboxylic acid N-hydroxysuccinimide (NHS) ester suitable for immediate conjugation on the synthesis column with molecules containing a primary amine, resulting in a stable amide linkage. PC Amino-Modifier is a photocleavable C6 amino-modifier, part of our line of photocleavable (PC) modifiers.If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200Thiol-Modifier C6 S-SThiol-Modifier C6 S-SGlen ResearchMaterial Safety Data SheetIf you cannot find the answer to your problem below then please contact us or telephone 01954 210 200Thiol-Modifier C6 S-SThiol-Modifier C6 S-SGlen ResearchFREQUENTLY ASKED TECHNICAL QUESTIONQUESTION: Do you have a biotin phosphoramidite containing a disulfide linker which can be cleaved later with DTT to release the DNA from a streptavidin support?RESPONSE:No. However, this can be produced on the synthesizer by adding to the 5"- terminus first 5"-thiol-modifier C6 S-S (10-1936) followed by BioTEG phosphoramidite (10-1955). This should generate a biotinylated primer with a long spacer arm containing the disulfide linkage which can be cleaved later with DTT.QUESTION: What is the best method to make peptide-oligonucleotide conjugates?RESPONSE:It would seem that the best method to make peptide-oligo conjugates would be to use Fmoc chemistry and synthesize the peptide off an oligo synthesized on amino-CPG. However, deprotection of peptides synthesized using Fmoc chemistry requires 50% TFA and t-boc synthesized peptides require HF both of which would severely damage if not completely hydrolyze the oligo.The best and most straight foward method is to use a heterobifunctional crosslinking reagent to link a synthetic peptide, containing an N-terminal lysine, to a 5"-Thiol modified oligo or conversely a 5"-amino modified oligo to a cysteine containing peptide . A good crosslinking reagent is N-Maleimido-6-aminocaproyl- (2"-nitro,4"-sulfonic acid)-phenyl ester . Na (mal-sac-HNSA) from Bachem Bioscience (cat. # Q-1615). Reaction of this crosslinker with an amino group releases the dianion phenolate, 1-hydroxy-2-nitro -4-benzene sulfonic acid a yellow chromophore. The chromophore allows both quantitation of the coupling reaction as well as act as an aid in monitoring the seperation of "activated peptide" from free crosslinking reagent using gel filtration.Method A: Couple Peptide Amine To Oligo Thiol (Note peptide MW must be > 5,000 to be excluded from desalting column). This method best for oligo-enzyme conjugation.Step 1: Synthesize a peptide with an N-terminal, or internal, lysine (The epsilon amino group is more reactive than an alpha amino group).Step 2: Synthesize an oligonucleotide with a 5" Thiol group.Step 3: React peptide with excess mal-sac-HNSA (pH 7.5 Sodium phosphate)Step 4: Seperation of peptide-mal-sac conjugate from free crosslinker and buffer exchange (pH 6.0 Sodium phosphate) using a gel filtration column (Glen Gel-Pak™ or eq.). Note peptide must be large enough to seperate from the free linker which can be visualized as a yellow band. Do not collect yellow band with peptide.Step 5: Activate thiol modified oligo, desalt and buffer exchange (pH 6 Sodium phosphate) on Glen Gel-Pak™ column.Step 6: React acitvated peptide with Thiol modified oligo.Step 7: Purify Peptide-Oligo conjugate by ion exchange chromatography on Nucleogen DEAE-500-10 or eq. Elution order: free peptide, peptide-oligo, free oligo.Method B: Couple Oligo Amine To Peptide Cysteine (Note oligos > 15mers are excluded from desalting column). Use above procedure switching oligo for peptide.Step 1: Synthesize a peptide with an N-terminal, or internal, cysteineStep 2: Synthesize an oligonucleotide with a 5" amino modifier.Step 3: Purify oligo Trityl-on by RP HPLC or cartridge.Step 4: React oligo with excess mal-sac-HNSA (pH 7.5 Sodium phosphate)Step 5: Seperation of oligo-mal-sac conjugate from free crosslinker and buffer exchange (pH 6 Sodium phosphate) using a gel filtration column (Glen Gel-Pak™ or eq.). Note oligo must be large enough to seperate from the free linker which can be visualized as a yellow band.Do not collect yellow band with oligo.Step 6: Dissolve peptide in pH 6.0 Sodium phosphate buffer and react with activated oligo.Step 7: Purify Peptide-Oligo conjugate by ion exchange chromatography on Nucleogen DEAE-500-10 or eq. Elution order: free peptide, peptide-oligo, free oligo.If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200Thiol-Modifier C6 S-SThiol-Modifier C6 S-SGlen ResearchDILUTION/COUPLING DATAThe table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.ABI 392/394Cat.No.PackSizeGrams/Pack0.1M Dil.(mL)LV40LV20040nm0.2µm1µm10µmApproximate Number of Additions10-1936-020.25grams.25grams3.25955735.6325.91194.7510-1936-90100µmoles.077grams120127.55.4541ExpediteCat.No.PackSizeGrams/PackDilution(mL)Molarity50nm0.2µm1µm15µmApproximate Number of Additions10-1936-020.25grams.25grams4.85.0790.656.6341.185.6610-1936-90100µmoles.077grams1.5.0723.614.7510.731.48BeckmanCat.No.PackSizeGrams/PackDilution(mL)Molarity30nm200nm1000nmApproximate Number of Additions10-1936-020.25grams.25grams4.85.0792.257.6341.9110-1936-90100µmoles.077grams1.5.0725.215.7511.45If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Athens Research & Technology—供应多款蛋白、酶制品

Athens Research & Technology是成立于2010年的新兴生物高科技制品公司，该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房，并且通过ISO 9001：2008认证。Athens公司致力于纯化分离高纯度，高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶，蛋白酶抑制剂，中性粒细胞酶，载脂蛋白，脂蛋白，血小板蛋白，转铁蛋白，免疫球蛋白等等。公司产品适用于炎症，冠状动脉疾病，自身免疫性疾病，癌症，阿尔茨海默氏病等众多研究领域，已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液（包括干细胞）等产品研发。除了人类蛋白质，我们还从动物血清和组织中分离多种蛋白质及酶类。此外，Athens Research and Technology还提供特殊试剂/蛋白定制服务，以满足研究人员的不同需求。