首页 > EZ Biosystems > EZ Biosystems/Avalanche®-CRISPR Transfection Reagent/0.5 ml/EZT-CRPR-1-0.5 ml

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EZ Biosystems/Avalanche®-CRISPR Transfection Reagent/0.5 ml/EZT-CRPR-1-0.5 ml

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EZT-CRPR-1-0.5ml

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CRISPR-Cas9–mediated genome targeting:CRISPR-Cas9 has triggered a revolution in which laboratories around the world are using the technology for innovative applications in biology. This technology is able to systematically analyze gene functions in mammalian cells, study genomic rearrangements and the progression of cancers or other diseases, and potentially correct genetic mutations responsible for inherited disorders.The CRISPR-Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as plasmids and phages, and provides a form of acquired immunity. CRISPR spacers recognize and cut these exogenous genetic elements in a manner analogous to RNA interference in eukaryotic organisms. CRISPRs are found in approximately 40% of sequenced bacteria genomes and 90% of sequenced archaea.The CRISPR interference technique can be applied in eukaryotic cells. By delivering the Cas9 protein and appropriate guide RNAs (sgRNA) into a cell, the organism's genome can be cut at any desired location.The Cas9 enzyme (green) generates breaks in double-stranded DNA by using its two catalytic centers to cleave each strand of a DNA target site next to a PAM sequence and matching the 20-nucleotide sequence of the single guide RNA (sgRNA).The Transfection Reagent:One of the most important factors that determines a successful CRISPR-Cas9–mediated genome targeting is the efficiency of delivering functional Cas9 gene and sgRNA into the cells (the transfection process). EZ Biosystems, the transfection and gene expression experts, has specifically developed the transfection reagent for CRISPR-Cas9 gene editing in mammalian cells ---- Avalanche®-CRISPR Transfection Reagent:Features:Specifically designed for CRISPR-Cas9 gene editing transfection in mammalian cells.Maximum efficiency for transfecting large plasmids (>9.0 kb)Great for co-transfection of large plasmids with either small plasmids or small RNA, such as sgRNAGreat for CRISPR-Cas9 gene editing in difficult-to-transfect cells, such as primary cells, suspension culture cells, or neural cells.

EZ生物系统™是一家全球性的基因转染和基因表达产品和服务提供商。

EZ Biosystems™ is a worldwide provider of transfection and gene expression products & services.
One of the major focuses of EZ Biosystems™ has been the research, development, and manufacture of next-generation transfection reagents ---- the Avalanche® Transfection Reagent series by applying our expertise in combinatorial chemistry, molecular biology, and cell biology.

品牌	货号	名称	规格
EZ Biosystems	EZT-CRPR-1	Avalanche®-CRISPR Transfection Reagent	0.5ml 1.5ml
EZ Biosystems	EZT-OMNI-1	Avalanche®-Omni Transfection Reagent	0.75ml 1.5ml 5*1.5ml
EZ Biosystems	EZT-EVDY-1	Avalanche®-Everyday Transfection Reagent	0.75ml 1.5ml