Cell Line Information:Designations: 293 [HEK-293]Depositors: FL GrahamGrowth Properties: adherentOrganism: Homo sapiensMorphology: epithelialSource:Organ: embryonic kidneyApplications: efficacy testing, transfection host, viruscide testingReceptors: vitronectinTumorigenic: YESAge: fetusComments: The cells containes Adenovirus 5 DNA from left ends of the viral genome. The line is excellent for titrating human adenoviruses. The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit. The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2)The Transfection Reagent:293 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on 293 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for 293 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of 293 Cell Avalanche™ Transfection Reagent for transfection on 293 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?References:Amini M, Wang H, Belkacemi A, Jung M, Bertl A, etal.Identification of Inhibitory Ca2+ Binding Sites in the Upper Vestibule of the Yeast Vacuolar TRP Channel. iScience. 2019 Jan 25;11:1-12. PubMed PMID: 30572205; PubMed Central PMCID: PMC6299153.Features:Specifically optimized to deliver nucleic acids into 293 CellsHighest efficiency to ensure experimental success Lowest Cellular Toxicity-maintain cell density and reduce experimental biases0.5 ml is able to transfect about 1000 wells of 24-well plateSimplest and straightforward protocolsDeliver single or multiple plasmidsCompatible with serum Suitable for Reverse Transfection Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results. Economical: High efficiency means less amount of nucleic acid & reagent is needed Developed and manufactured by EZ Biosystems

EZ生物系统™是一家全球性的基因转染和基因表达产品和服务提供商。

EZ Biosystems™ is a worldwide provider of transfection and gene expression products & services.
One of the major focuses of EZ Biosystems™ has been the research, development, and manufacture of next-generation transfection reagents ---- the Avalanche® Transfection Reagent series by applying our expertise in combinatorial chemistry, molecular biology, and cell biology.