首页 > StressMarq > StressMarq/StressXpress® 20S Proteasome Activity Kit/SKT-133-96/96-well

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StressMarq/StressXpress® 20S Proteasome Activity Kit/SKT-133-96/96-well

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Overview:ProductName20SProteasomeActivityKitDescriptionFluorometricdetectionofpurified20SproteasomeactivitySpeciesReactivitySpeciesIndependentPlatformMicroplateSampleTypesPurifiedProteinsDetectionMethodFluorometricAssayAssayTypeContinuousKineticAssayUtilityActivitykitusedtoscreenpotentialproteasomeinhibitorsandactivators,andtomeasureandquantify20Sproteasomeactivityincontinuouskineticandend-pointassays.IncubationTime15-30minutesNumberofSamples40samplesinduplicateusingtheplateformat,or100x50µLmicro-cuvettebasedassaysOtherResourcesKitBooklet,MSDSPropertiesStorageTemperature4ºCShippingTemperatureBlueIceProductTypeActivityKitsAssayOverviewThe20SProteasomeActivitykitfacilitatestherapid,robustmeasurementofproteasomeactivityinvitro.Thekitutiliseshighpurity,fluorogenicsubstrateSuc-LLVY-AMCtogetherwithsuitablecalibrationstandardsandcontrolsfortheaccurateandsensitiveassessmentofproteasomechymotrypsin-like(β5)activity.Continuouskineticorend-pointassayscanbeperformedinmicro-cuvettesorin96-wellplateformatformulti-sampleanalysis.Containssufficientmaterialsforonefull96-wellplateassayorupto100x50µLmicro-cuvettebasedassaystoberun.KitOverviewComponentNo.ItemQuantity/SizeSKC-133A10xStressXpress®proteasomeassaybuffer1ml(reconstituted)SKC-133B20Sproteasome10µgSKC-133CMG132(controlinhibitor)100µgSKC-133DStressXpress®96wellassayplate1plateSKC-133EStressXpress®AMCstandard1.75µgSKC-133FSuc-LLVY-AMC(chymotrypsin-likesubstrate)500µgCiteThisProduct20SProteasomeActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-133)BIOLOGicalDescriptionAlternativeNamesChymotrypsin-like(β5)ActivityKitResearchAreasCancer,Apoptosis,CardiovascularSystem,CellSignaling,Neurodegeneration,Neuroscience,Post-translationalModificationsScientificBackgroundProteasomesarenon-lysosomalproteolyticcomplexeslocalisedprimarilyinthecytoplasmandinthenucleusofeukaryoticcells.TheyareresponsIBLefortheubiquitin-mediateddegradationofshorthalf-lifeproteinsandpeptidesthatareinvolvedinessentialcellularprocessesincludingcell-cycleregulation,apoptosisandtranscriptionalregulation,innateimmunityandantigenprocessing,andintheremovalofredundantordamagedproteins.Assuchproteindegradationbytheubiquitin-proteasomepathwayhasamajorregulatoryfunctionforproliferationactivityandsurvivalofbothnormalandmalignantcells,anditsdysfunctionhasbeenimplicatedinawiderangeofotherdiseaseprocessesincludingneurodegenerative,cardiovascularandmetabolicdisorders.The26Sproteasomestructureiscomposedofa20Sproteasomecatalyticcorecomplexandoneortwo19S(PA700)regulatorysubcomplexes.The20Scorecomprisestwocopiesof14subunits(7alphasubunitsand7betasubunits)arrangedinaα7β7β7α7cylindricalarray.Proteolyticactivitiesaredeterminedbytheβ1(caspase-like),β2(trypsin-like)andβ5(chymotrypsin-like)subunits,accesstowhichisguardedbytheα-subunits.The19SregulatoryunitconsistsofsixATPaseandatleasttennon-ATPasesubunitsthatarerequiredforubiquitinatedproteinbinding,deubiquitination,substrateunfoldingandtranslocationtothe20Scatalyticcore.Varyingcatalyticsubunitcomposition(β1,β1i;β2,β2i;β5,β5i)resultsinavarietyofpossiblesubtypesfromfullconstitutiveproteasome(β1,β2,β5)throughmixedpopulationstofullinducible/immunoproteasome(β1i,β2i,β5i).AlternativeregulatorycomplexessuchasthePA200and11Sproteasomeactivatorsconferdifferentsubstratespecificitiesandactivitycomparedtothe19Sregulator.References1.Saeki,Y.&Tanaka,K.Methodsinmolecularbiology(Clifton,N.J.)832,315–37(2012).2.Frankland-Searby,S.&Bhaumik,S.R.Biochimicaetbiophysicaacta1825,64–76(2012).3.Basler,M.,Kirk,C.J.&Groettrup,M.Currentopinioninimmunology1–7(2012).4.Löw,P.Generalandcomparativeendocrinology172,39–43(2011).5.Dennissen,F.J.a,Kholod,N.&VanLeeuwen,F.W.Progressinneurobiology96,190–207(2012).6.Geng,F.,Wenzel,S.&Tansey,W.P.Annualreviewofbiochemistry81,177–201(2012).ProductImagesAMCStandardCurvefor20SProteasomeActivityKit.Representativeplotoffluorescencemeasurements(RFU)ofserialdilutionsofAMCStandardatrecommendedconcentrations.Continuouskineticenzymeactivityassayshowingthetimecourseofcontrol20SproteasomeactivitywithSuc-LLVY-AMCsubstrateusingtheStressXpress®20SProteasomeActivityKit.20Sproteasome(~2.5nM)wasincubatedwith100µMSuc-LLVY-AMCinproteasomeassaybufferfor30minutesatroomtemperaturealongsideasubstrateonly(blank)controlreaction.Fluorescencemeasurements(RFU)weretakenat30secondintervalsandplottedvs.time.ProductCitations(0)Currentlytherearenocitationsforthisproduct.

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。