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StressMarq/Anti-Abl1 Antibody (pTyr264)/SPC-904D-HRP/100-µl
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Overview:ProductNameAbl1Antibody(pTyr264)DescriptionRabbitAnti-HumanAbl1(pTyr264)PolyclonalSpeciesReactivityHumanApplicationsWB,AMAntibodyDilutionWB(1:250);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesRabbitImmunogenSpeciesHumanImmunogenAphospho-specificpeptidecorrespondingtoresiduessurroundingTyr264ofhumanAbl1-pY469(AA466-472)ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,UnconjugatedPropertiesStorageBufferPBSpH7.4,50%glycerol,0.025%ThimerosalStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationPeptideAffinityPurifiedClonalityPolyclonalSpecificityDetects122.945kDa.CiteThisProductStressMarqBiosciencesCat#SPC-904,RRID:AB_2706671CertificateofAnalysisA1:250dilutionofSPC-904wassufficientfordetectionofAbl1(pTyr264)in10µgofHeLacelllysatebyECLimmunoblotanalysisusinggoatanti-rabbitIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesAbelsontyrosineproteinkinase1Antibody,ABLAntibody,cABLAntibody,p150Antibody,bcr/ablAntibodyResearchAreasCancer,CellSignaling,EpigeneticsandNuclearSignaling,Oncoproteins,ProteinPhosphorylation,Transcription,TumorBioMarkers,TyrosineKinasesCellularLocalizationCytoplasm,Cytoskeleton,Mitochondrion,NucleusAccessionNumberNP_005148GeneID25SwissProtP00519ScientificBackgroundABL1isawidely-expressed,non-receptorprotein-tyrosinekinase.ItisstABIlizedintheinactiveformbyanassociationbetweentheSH3domainandtheSH2-TKlinkerregion,interactionsoftheN-terminalcap,andcontributionsfromanN-terminalmyristoylgroupandphospholipids.ItisactivatedbyautophosphorylationaswellasbySrc-familykinase-mediatedphosphorylation.ItcanalsobeactivatedbyRIN1bindingtotheSH2andSH3domains.AsitisstimulatedbycelldeathinducersandDNA-damage,itislikelythatthewild-typeformofAbl1isactuallyatumour-suppressorprotein.ThenormalactiveformofAbl1isnuclear,anditissequesteredintothecytoplasmbyinteractionwith14-3-3throughThr-735.Itlocalizestothemitochondriaunderconditionsofoxidativestress.ProductImagesCurrentlytherearenoimagesforthisproductProductCitations(0)Currentlytherearenocitationsforthisproduct.HRP(HorserADIshperoxidase)Properties:EnzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatargetReadilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonorsCatalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproductsChemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproductsHighturnoverrateenablesrapidgenerationofastrongsignal44kDaglycoproteinExtinctioncoefficient:100(403nm)Applications:Westernblot,immunohistochemistry,andELISAHRPDatasheet
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。
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