Overview:ProductNameATG12AntibodyDescriptionRabbitAnti-HumanATG12PolyclonalSpeciesReactivityHumanApplicationsWB,ICC/IFAntibodyDilutionWB(1:1000);ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesRabbitImmunogenSpeciesHumanImmunogenSyntheticpeptidefromtheC-terminalofhumanATG12Concentration1mg/mlConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated PerCP Overview:Peridinin-Chlorophyll-ProteinComplexSmallphycobiliproteinIsolatedfromredalgaeLargestokesshift(195nm)MolecularWeight:35kDaPerCPDatasheet OpticalProperties:λex =482nmλem=677nmεmax=1.96x106Laser =488nm  PE/ATTO594PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.Overview:HighfluorescenceyieldHighphotostABIlityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationPE/ATTO594DatasheetOpticalProperties:λex =535nmλem =627 nmLaser = 488to561nm  FITC(Fluorescein)Overview:ExcellentfluorescencequantumyieldHighrateofphotobleachingGoodsolubilityinwaterBroademissionspectrumpHdependentspectraMolecularformula:C20H12O5Molarmass:332.3g/molFITC-Fluorescent-conjugateOpticalProperties:λex =494 nmλem=520 nmεmax=7.3×104Φf= 0.92τfl =5.0nsBrightness= 67.2Laser = 488 nmFilterset = FITC  ATTO700Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:575g/molATTO700Datasheet OpticalProperties:λex =700 nmλem=719nmεmax=1.25×105Φf= 0.25τfl =1.6nsBrightness= 31.3Laser =676nmFilterset =Cy®5.5  ATTO680Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:631g/molATTO680Datasheet OpticalProperties:λex =680nmλem=700 nmεmax=1.25×105Φf= 0.30τfl =1.7nsBrightness=37.5Laser =633 –676nmFilterset =Cy®5.5  ATTO655Overview:HighfluorescenceyieldHighthermalandphotostabilityExcellentozoneresistanceQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:634g/molATTO655DatasheetOpticalProperties:λex =663nmλem=684nmεmax=1.25×105Φf= 0.30τfl =1.8nsBrightness= 37.5Laser =633 –647nmFilterset =Cy®5  ATTO633Overview:HighfluorescenceyieldHighthermalandphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsStableatpH4–11Cationicdye,perchloratesaltMolarMass:652.2g/molATTO633DatasheetOpticalProperties:λex =629nmλem=657nmεmax=1.3×105Φf= 0.64τfl =3.2nsBrightness=83.2Laser =633 nmFilterset =Cy®5  ATTO594Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:1137g/molATTO594Datasheet OpticalProperties:λex =601 nmλem=627nmεmax=1.2×105Φf= 0.85τfl =3.5nsBrightness=102Laser =594nmFilterset =TexasRed®  ATTO565Overview:HighfluorescenceyieldHighthermalandphotostabilityGoodsolubilityinpolarsolventsExcellentsolubilityinwaterVerylittleaggregationRhodaminedyederivativeMolarMass:611g/molATTO565Datasheet OpticalProperties:λex =563nmλem=592nmεmax=1.2×105Φf= 0.9τfl =3.4nBrightness=10Laser =532nmFilterset = TRITC  ATTO488Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:804g/mol ATTO488Datasheet  OpticalProperties:λex =501nmλem=523nmεmax=9.0×104Φf= 0.80τfl =4.1nsBrightness= 72Laser = 488 nmFilterset = FITC  ATTO390Overview:HighfluorescenceyieldLargeStokes-shift(89nm)GoodphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsCoumarinderivate,unchargedLowmolarmass:343.42g/mol ATTO390DatasheetOpticalProperties:λex =390nmλem=479nmεmax=2.4×104Φf= 0.90τfl =5.0nsBrightness= 21.6Laser =365or405nm APC(Allophycocyanin)Overview:HighquantumyieldLargephycobiliprotein6chromophorespermoleculeIsolatedfromredalgaeMolecularWeight:105kDaAPCDatasheet OpticalProperties:λex =650nmλem=660nmεmax=7.0×105Φf= 0.68Brightness= 476Laser =594or633 nmFilterset =Cy®5 StreptavidinProperties:Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinityMolecularweight:53kDaFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISAStreptavidinDatasheetBiotinProperties:BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinityMolarmass:244.31g/molFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISABiotinDatasheetHRP(HorserADIshperoxidase)Properties:EnzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatargetReadilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonorsCatalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproductsChemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproductsHighturnoverrateenablesrapidgenerationofastrongsignal44kDaglycoproteinExtinctioncoefficient:100(403nm)Applications:Westernblot,immunohistochemistry,andELISAHRPDatasheetAP(AlkalinePhosphatase)Properties:Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenolsCommonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligationCatalyzestheconversionof:Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproductsFluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproductsMolecularweight:140kDaApplications:Westernblot,immunohistochemistry,andELISAAPDatasheet R-PE(R-Phycoerythrin)Overview:BroadexcitationspectrumHighquantumyieldPhotostableMemberofthephycobiliproteinfamilyIsolatedfromredalgaeExcellentsolubilityinwaterMolecularWeight:250kDaR-PEDatasheet OpticalProperties:λex =565nmλem=575nmεmax=2.0×106Φf= 0.84Brightness=1.68x103Laser = 488to561nmFilterset = TRITC PropertiesStorageBufferPBS,50%glycerol,0.09%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationPeptideAffinityPurifiedClonalityPolyclonalSpecificityPredictedmolecularweightat~15.1kDa.Observedmolecularweightsat~48-55kDabasedonATG12-ATG5heterodimer.CiteThisProductStressMarqBiosciencesCat#SPC-608,RRID:AB_2704853CertificateofAnalysisA1:1000dilutionofSPC-608wassufficientfordetectionofATG12onHeLacelllysatesusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesAPG12-likeAntibody,APG12Antibody,ATG12_HumanAntibody,HAPG12Antibody,FBR93AntibodyResearchAreasCancer,Autophagy,CardiovascularSystem,CellSignaling,EpigeneticsandNuclearSignaling,HeartCellularLocalizationCytoplasmAccessionNumberNP_001264712.1GeneID9140SwissProtO94817ScientificBackgroundATG12(autophagy-relatedprotein12),alsoknownasAPG12,isa140aminoacidproteinthatisubiquitouslyexpressedandbelongstotheATG12familyofproteins.ATG12isahomologoftheyeastproteinAPG12thatparticipatesinautophagy.Autophagyisamembranetraffickingmechanismthatdeliverscytoplasmiccargotothevacuole/lysosomefordegradationandrecycling.Inyeast,autophagyrequiresaproteinconjugationsystemconsistingofAPG12covalentlyboundatthecarboxyterminalglycinetolysine149ofAPG5.Similarlyinhumans,ATG12isessentialforautophagyandlocalizestothecytoplasmwhereitiscovalentlyboundtoAPG5,aconjugationreactionthatrequiresAPG7,ATG10andATP(1-3).TheATG12-APG5conjugatefunctionsasanimportantregulatoroftheautophagicprocessandisrequiredforthechangeinmembranemorphologyanddevelopmentofautophagosomes.Duetoalternativesplicingevents,twoATG12isoformsexist.References1.MizushimaN.,SugitaH.,YoshimoriT.,OhsumiY.(1999)JBiolChem.273(51):33889-33892.2.MizushumaN.,etal.(1998)Nature.395:395-398.3.SuzukiK.,etal.(2001)EMBOJ.20:5971-5981.ProductImagesImmunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-ATG12PolyclonalAntibody(SPC-608).Tissue:CervicalCancercellline(HeLa).Species:Human.Fixation:4%Formaldehydefor15minatRT.PrimaryAntibody:RabbitAnti-ATG12PolyclonalAntibody(SPC-608)at1:100for60minatRT.SecondaryAntibody:GoatAnti-RabbitATTO488at1:100for60minatRT.Counterstain:DAPI(blue)nuclearstainat1:5000for5minRT.Localization:Nucleus,Cytoplasm.Magnification:40X.(A)DAPI(blue)nuclearstain(B)PhalloidinTexasRedF-Actinstain(C)ATG12Antibody(D)Composite.WesternblotanalysisofHumanHeLacelllysatesshowingdetectionof~15kDaATG12proteinusingRabbitAnti-ATG12PolyclonalAntibody(SPC-608).Lane1:MWLadder.Lane2:HumanHeLa(20µg).Load:20µg.Block:5%milk+TBSTfor1houratRT.PrimaryAntibody:RabbitAnti-ATG12PolyclonalAntibody(SPC-608)at1:1000for1houratRT.SecondaryAntibody:GoatAnti-Rabbit:HRPat1:2000for1houratRT.ColorDevelopment:TMBsolutionfor12minatRT.Predicted/ObservedSize:~15kDa.OtherBand(s):ATG12-ATG5Complex.ProductCitations(0)Currentlytherearenocitationsforthisproduct. ATTO680Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:631g/molATTO680Datasheet OpticalProperties:λex =680nmλem=700 nmεmax=1.25×105Φf= 0.30τfl =1.7nsBrightness=37.5Laser =633 –676nmFilterset =Cy®5.5