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StressMarq/Anti-BRSK1 Antibody (pThr189)/SPC-927D-A680/100-µl
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产品说明
Overview:
Product Name
BRSK1 Antibody (pThr189)
DescriptionRabbit Anti-Human BRSK1 (pThr189) Polyclonal
Species Reactivity
Human
Applications
WB, AM
Antibody Dilution
WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species
Rabbit
Immunogen Species
Human
Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr189 of human BRSK1 (AA186-192)
Conjugates
Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
Properties
Storage Buffer
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature
-20ºC
Shipping Temperature
Blue Ice or 4ºC
Purification
Peptide Affinity Purified
Clonality
Polyclonal
Specificity
Detects 86.753 kDa.
Cite This Product
StressMarq Biosciences Cat# SPC-927, RRID: AB_2707085
Certificate of Analysis
A 1:250 dilution of SPC-927 was sufficient for detection of BRSK1 (pThr189) in 10 µg of human brain lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Biological Description
Alternative Names
Brain selective kinase 1 Antibody, Brain-specific serine/threonine-protein kinase 1 Antibody, BRSK 1 Antibody, hSAD1 Antibody, SAD1 Antibody, SADB Antibody, Serine/threonine kinase SAD B Antibody
Research Areas
Cancer, Cell Cycle, Cell Signaling, Protein Phosphorylation
Cellular Localization
Cytoplasm, Nucleus
Accession Number
NP_115806
Gene ID
84446
Swiss Prot
Q8TDC3
Scientific Background
BRSK1 (BR serine/threonine-protein kinase 1), also known as SAD1, is a 794 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one UBA domain and one protein kinase domain. Expressed in a variety of tissues with highest expression in testis and brain, BRSK1 uses magensium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins, including Wee 1 and Cdc25B. Via its kinase activity toward proteins that are involved in microtubule assembly, BRSK1 plays an essential role in neuronal polarization and may be involved in regulating cell cycle arrest in response to DNA damage, Two isoforms of BRSK1 exist due to alternative splicing events.
Product Images
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Product Citations (0)
Currently there are no citations for this product. ATTO 680Overview:High fluorescence yieldExcellent thermal and photostabilityQuenched by electron donorsVery hydrophilicGood solubility in polar solventsZwitterionic dyeMolar Mass: 631 g/molATTO 680 Datasheet Optical Properties:λex = 680 nmλem = 700 nmεmax = 1.25×105Φf = 0.30τfl = 1.7 nsBrightness = 37.5Laser = 633 – 676 nmFilter set = Cy®5.5
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。
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