Overview:ProductNameCav1.3AntibodyDescriptionMouseAnti-RatCav1.3MonoclonalIgG2aKappaSpeciesReactivityHuman,Mouse,RatApplicationsWB,IHC,ICC/IF,IP,AMAntibodyDilutionWB(1:1000),IHC(1:1000),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesMouseImmunogenSpeciesRatImmunogenFusionproteinaminoacids859-875ofratCav1.3Concentration1mg/mlConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated PerCP Overview:Peridinin-Chlorophyll-ProteinComplexSmallphycobiliproteinIsolatedfromredalgaeLargestokesshift(195nm)MolecularWeight:35kDaPerCPDatasheet OpticalProperties:λex =482nmλem=677nmεmax=1.96x106Laser =488nm  PE/ATTO594PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.Overview:HighfluorescenceyieldHighphotostABIlityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationPE/ATTO594DatasheetOpticalProperties:λex =535nmλem =627 nmLaser = 488to561nm  FITC(Fluorescein)Overview:ExcellentfluorescencequantumyieldHighrateofphotobleachingGoodsolubilityinwaterBroademissionspectrumpHdependentspectraMolecularformula:C20H12O5Molarmass:332.3g/molFITC-Fluorescent-conjugateOpticalProperties:λex =494 nmλem=520 nmεmax=7.3×104Φf= 0.92τfl =5.0nsBrightness= 67.2Laser = 488 nmFilterset = FITC  ATTO700Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:575g/molATTO700Datasheet OpticalProperties:λex =700 nmλem=719nmεmax=1.25×105Φf= 0.25τfl =1.6nsBrightness= 31.3Laser =676nmFilterset =Cy®5.5  ATTO680Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:631g/molATTO680Datasheet OpticalProperties:λex =680nmλem=700 nmεmax=1.25×105Φf= 0.30τfl =1.7nsBrightness=37.5Laser =633 –676nmFilterset =Cy®5.5  ATTO655Overview:HighfluorescenceyieldHighthermalandphotostabilityExcellentozoneresistanceQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:634g/molATTO655DatasheetOpticalProperties:λex =663nmλem=684nmεmax=1.25×105Φf= 0.30τfl =1.8nsBrightness= 37.5Laser =633 –647nmFilterset =Cy®5  ATTO633Overview:HighfluorescenceyieldHighthermalandphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsStableatpH4–11Cationicdye,perchloratesaltMolarMass:652.2g/molATTO633DatasheetOpticalProperties:λex =629nmλem=657nmεmax=1.3×105Φf= 0.64τfl =3.2nsBrightness=83.2Laser =633 nmFilterset =Cy®5  ATTO594Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:1137g/molATTO594Datasheet OpticalProperties:λex =601 nmλem=627nmεmax=1.2×105Φf= 0.85τfl =3.5nsBrightness=102Laser =594nmFilterset =TexasRed®  ATTO565Overview:HighfluorescenceyieldHighthermalandphotostabilityGoodsolubilityinpolarsolventsExcellentsolubilityinwaterVerylittleaggregationRhodaminedyederivativeMolarMass:611g/molATTO565Datasheet OpticalProperties:λex =563nmλem=592nmεmax=1.2×105Φf= 0.9τfl =3.4nBrightness=10Laser =532nmFilterset = TRITC  ATTO488Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:804g/mol ATTO488Datasheet  OpticalProperties:λex =501nmλem=523nmεmax=9.0×104Φf= 0.80τfl =4.1nsBrightness= 72Laser = 488 nmFilterset = FITC  ATTO390Overview:HighfluorescenceyieldLargeStokes-shift(89nm)GoodphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsCoumarinderivate,unchargedLowmolarmass:343.42g/mol ATTO390DatasheetOpticalProperties:λex =390nmλem=479nmεmax=2.4×104Φf= 0.90τfl =5.0nsBrightness= 21.6Laser =365or405nm APC(Allophycocyanin)Overview:HighquantumyieldLargephycobiliprotein6chromophorespermoleculeIsolatedfromredalgaeMolecularWeight:105kDaAPCDatasheet OpticalProperties:λex =650nmλem=660nmεmax=7.0×105Φf= 0.68Brightness= 476Laser =594or633 nmFilterset =Cy®5 StreptavidinProperties:Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinityMolecularweight:53kDaFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISAStreptavidinDatasheetBiotinProperties:BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinityMolarmass:244.31g/molFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISABiotinDatasheetHRP(HorserADIshperoxidase)Properties:EnzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatargetReadilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonorsCatalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproductsChemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproductsHighturnoverrateenablesrapidgenerationofastrongsignal44kDaglycoproteinExtinctioncoefficient:100(403nm)Applications:Westernblot,immunohistochemistry,andELISAHRPDatasheetAP(AlkalinePhosphatase)Properties:Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenolsCommonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligationCatalyzestheconversionof:Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproductsFluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproductsMolecularweight:140kDaApplications:Westernblot,immunohistochemistry,andELISAAPDatasheet R-PE(R-Phycoerythrin)Overview:BroadexcitationspectrumHighquantumyieldPhotostableMemberofthephycobiliproteinfamilyIsolatedfromredalgaeExcellentsolubilityinwaterMolecularWeight:250kDaR-PEDatasheet OpticalProperties:λex =565nmλem=575nmεmax=2.0×106Φf= 0.84Brightness=1.68x103Laser = 488to561nmFilterset = TRITC PropertiesStorageBufferPBSpH7.4,50%glycerol,0.09%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationProteinGPurifiedClonalityMonoclonalCloneNumberS48A-9IsotypeIgG2aKappaSpecificityDetects~250kDa.Nocross-reactivityagainstCav1.2.CiteThisProductStressMarqBiosciencesCat#SMC-301,RRID:AB_2069096CertificateofAnalysis1µg/mlofSMC-301wassufficientfordetectionofCav1.3in10µgofratbrainlysatebycolorimetricimmunoblotanalysisusingGoatanti-mouseIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesalpha-1polypeptideAntibody,CAC1D_HUMANAntibody,CACH3Antibody,CACN4Antibody,CACNA1DAntibody,Cacna1dAntibody,CACNL1A2Antibody,CalciumchannelAntibody,CalciumchannelLtypealpha1polypeptideisoform2Antibody,Calciumchannelneuroendocrine/braintypealpha1subunitAntibody,CalciumchannelvoltagedependentLtypealpha1DsubunitAntibody,CCHL1A2Antibody,isoform2Antibody,LtypeAntibody,VoltagedependentLtypecalciumchannelsubunitalpha1DAntibody,Voltagegatedcalciumchannelalpha1subunitAntibody,VoltagegatedcalciumchannelalphasubunitCav1.3Antibody,VoltagegatedcalciumchannelsubunitalphaCav1.3Antibody,Voltage-dependentL-typecalciumchannelsubunitalpha-1DAntibody,Voltage-gatedcalciumchannelsubunitalphaCav1.3AntibodyResearchAreasCancer,CalciumChannels,CellSignaling,IonChannels,Neuroscience,Voltage-GatedCalciumChannelsCellularLocalizationCellmembrane,MembraneAccessionNumberNP_058994.1GeneID29716SwissProtP27732ScientificBackgroundCaV1.3,alsoknownasthecalciumchannel,voltage-dependent,Ltype,alpha1Dsubunit(CACNA1D),isahumangene.CaV1.3subunitsareprimarilyexpressedinneuronsandneuroendocinecells.SomestudiessuggesthoweverthatCaV1.3isalsofoundintheatria,andmayfigureprominentlyinatrialarrhythmias(1).CaV1.3alsocarriestheprimarysensoryreceptorsofthemammaliancochlea,andarealsoexpressedintheelectromotileouterhaircells(2).References1.ZhangZ.,etal.(2005)Circulation112:1936-1944.2.JohnsonS.L.andMarcottiW.(2008)TheJournalofPhysiology.586:1029-1042.ProductImagesImmunohistochemistryanalysisusingMouseAnti-CaV1.3CalciumChannelMonoclonalAntibody,CloneS48A-9(SMC-301).Tissue:backskin.Species:Mouse.Fixation:Bouin’sFixativeandparaffin-embedded.PrimaryAntibody:MouseAnti-CaV1.3CalciumChannelMonoclonalAntibody(SMC-301)at1:100for1houratRT.SecondaryAntibody:FITCGoatAnti-Mouse(green)at1:50for1houratRT.ImmunohistochemistryanalysisusingMouseAnti-CaV1.3CalciumChannelMonoclonalAntibody,CloneS48A-9(SMC-301).Tissue:hippocampus.Species:Human.Fixation:Bouin’sFixativeandparaffin-embedded.PrimaryAntibody:MouseAnti-CaV1.3CalciumChannelMonoclonalAntibody(SMC-301)at1:1000for1houratRT.SecondaryAntibody:FITCGoatAnti-Mouse(green)at1:50for1houratRT.ProductCitations(2)OtherCitationsBioMarkerAnalysiswithGratingCoupledSurfacePlasmonCoupledFluorescence.Mendoza,A.,Dias,J.A.,Zeltner,T.andLawrence,D.A.(2014)JAdvBio&Biotech.1(1):1-22.PubMedID:N/AReactivityHumanApplications:AntibodyMicroarrayBiomarkerAnalysiswithGratingCoupledSurfacePlasmonCoupledFluorescence.Mendoza,A.,Dias,J.A.,Zeltner,T.andLawrence,D.A.(2014)JAdvBio&Biotech.1(1):1-22.PubMedID:N/AReactivityMouseApplications:AntibodyMicroarray PE/ATTO594PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationPE/ATTO594DatasheetOpticalProperties:λex =535nmλem =627 nmLaser = 488to561nm