首页 > StressMarq > StressMarq/Anti-CDC25C Antibody/SPC-1113D-HRP/100-µl

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StressMarq/Anti-CDC25C Antibody/SPC-1113D-HRP/100-µl

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SPC-1113D-HRP

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Overview: Product Name CDC25C Antibody DescriptionRabbit Anti-Human CDC25C Polyclonal Species Reactivity Human, Mouse Applications WB, AM Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user. Host Species Rabbit Immunogen Species Human Immunogen Synthetic peptide of human CDC25C (AA275-290) Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated Properties Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal Storage Temperature -20ºC Shipping Temperature Blue Ice or 4ºC Purification Peptide Affinity Purified Clonality Polyclonal Specificity Detects 53.365 kDa. Cite This Product StressMarq Biosciences Cat# SPC-1113, RRID: AB_2710433 Certificate of Analysis A 1:250 dilution of SPC-1113 was sufficient for detection of CDC25C in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. Biological Description Alternative Names CDC 25 antibody, Cdc 25C antibody, CDC25 antibody, CDC25C antibody, Cell division cycle 25 homolog C antibody, Cell division cycle 25C antibody, Dual specificity phosphatase Cdc25C antibody, M phase inducer phosphatase 3 antibody, Mitosis inducer CDC25 antibody, MPIP3 antibody, MPIP3_HUMAN antibody, Phosphotyrosine phosphatase antibody, PPP1R60 antibody Cellular Localization Nucleus Accession Number NP_001781 Gene ID 995 Swiss Prot P30307 Scientific Background The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by Tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while Tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 Tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a Tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other Tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences. Product Images Currently there are no images for this product Product Citations (0) Currently there are no citations for this product.HRP (Horseradish peroxidase)Properties:Enzymatic activity is used to amplify weak signals and increase visibility of a targetReadily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donorsCatalyzes the conversion of:Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured productsChemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent productsHigh turnover rate enables rapid generation of a strong signal44 kDa glycoproteinExtinction coefficient: 100 (403 nm)Applications: Western blot, immunohistochemistry, and ELISAHRP Datasheet

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。