Overview:ProductNameCox2AntibodyDescriptionRabbitAnti-HumanCox2PolyclonalSpeciesReactivityHuman,Mouse,RatApplicationsWB,IHC,ICC/IF,ELISAAntibodyDilutionWB(1:1000),IHC(1:100),ICC/IF(1:500),ELISA(1:20000);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesRabbitImmunogenSpeciesHumanImmunogenSynthesizedpeptidederivedfromhumanCox2.ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,UnconjugatedPropertiesStorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationAffinityPurifiedClonalityPolyclonalIsotypeIgGSpecificityDetectsendogenouslevelsoftotalCox2protein.CiteThisProductStressMarqBiosciencesCat#SPC-1321,RRID:AB_2713565CertificateofAnalysisA1:1000dilutionofSPC-1321wassufficientfordetectionofCox2in10µgofA549celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesCOX2antibody,COX3antibody,Cyclooxygenase1antibody,PCOX1antibody,PGG/HSantibody,PGHsynthase2antibody,PGHS1antibody,PHSIIantibody,Prostaglandinendoperoxidesynthase1antibody,Prostaglandinendoperoxidesynthase2antibody,PTGS2antibody,TIS10antibodyCellularLocalizationEndoplasmicreticulummembrane,MicrosomeMembraneAccessionNumberNP_000954.1GeneID5743SwissProtP35354ScientificBackgroundCyclooxygenase(COX),alsoknownasProstaglandinH2synthaseandProstaglandinendoperoxidesynthase,isakeyenzymeintheofconversionarachidonicacidtoProstaglandinH2.ProstaglandinH2isconvertedbyotherenzymesintoinflammatorymediatorsprostaglandin(PG)D2,PGE2,PGF2a,PGI2andThromboxaneA2.Thus,COXisakeyenzymeintheproductionofinflammatoryagentsandisthetargetofintenseresearchanddrugdiscoveryactivities.TherearetwoenzymesofCOX,COX1andCOX2.COX2isfoundinelevatedlevelsinsynoviocytesfromrheumatoidarthritispatients.ThediscomfortsofinflammationsuchaspainandswellingarelargelyduetotheactionofprostaglandinsproducedbyCOX-2.COX2(Cyclooxygenase2)isaninducIBLeenzyme.Itisinvolvedintheresponseofcellstogrowthfactors,tumorpromoters,andcytokinesthatinduceitsexpression.Givenitsroleinsynthesizingprostaglandins,COX2isthereforeofinterestinstudyingimmuneresponseregulation.COX2isinducedbyawidevarietyofstimuliandwasinitiallyidentifiedasimmediateearlygrowthresponsegene.Inaddition,COX2expressionmarkedlyincreasedin85-90%ofhumancolorectaladenocarcinomawhereasCOX1levelsremainunchanged.References1.JonesD.A.etal.(1993)J.Biol.Chem.268:9049-9054.2.KosakaT.etal.(1994)Eur.J.Biochem.221:889-897.3.ApplebyS.B.etal.(1994)Biochem.J.302:723-727.4.BarnettJ.etal.(1994)Biochim.Biophys.Acta1209:130-139.ProductImagesImmunohistochemistryanalysisusingRabbitAnti-Cox2PolyclonalAntibody(SPC-1321).Tissue:BreastCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-Cox2PolyclonalAntibody(SPC-1321)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-Cox2PolyclonalAntibody(SPC-1321).Tissue:A549.Species:Human.PrimaryAntibody:RabbitAnti-Cox2PolyclonalAntibody(SPC-1321)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.WesternblotanalysisofHumanA549celllysatesshowingdetectionof~74kDaCox2proteinusingRabbitAnti-Cox2PolyclonalAntibody(SPC-1321).Lane1:HumanA549.Lane2:HumanA549treatedwiththeimmunizingpeptide.PrimaryAntibody:RabbitAnti-Cox2PolyclonalAntibody(SPC-1321)at1:1000.Predicted/ObservedSize:~74kDa.ProductCitations(0)Currentlytherearenocitationsforthisproduct.BiotinProperties:BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinityMolarmass:244.31g/molFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISABiotinDatasheet