Overview:ProductNameGFAPR416WTAntibodyDescriptionMouseAnti-HumanGFAPR416WTMonoclonalIgG1SpeciesReactivityHuman,Mouse,RatApplicationsWB,IHC,ICC/IFAntibodyDilutionWB(1:1000);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesMouseImmunogenSpeciesHumanImmunogenSyntheticpeptideaminoacids411-422(KTVEMRDGEVIK)ofhumanGFAP;100%identicalinratandmouse.>50%identitywithotherproteins(Vimentin,DesminandPeripherin).Concentration1mg/mlConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated PerCP Overview:Peridinin-Chlorophyll-ProteinComplexSmallphycobiliproteinIsolatedfromredalgaeLargestokesshift(195nm)MolecularWeight:35kDaPerCPDatasheet OpticalProperties:λex =482nmλem=677nmεmax=1.96x106Laser =488nm  PE/ATTO594PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.Overview:HighfluorescenceyieldHighphotostABIlityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationPE/ATTO594DatasheetOpticalProperties:λex =535nmλem =627 nmLaser = 488to561nm  FITC(Fluorescein)Overview:ExcellentfluorescencequantumyieldHighrateofphotobleachingGoodsolubilityinwaterBroademissionspectrumpHdependentspectraMolecularformula:C20H12O5Molarmass:332.3g/molFITC-Fluorescent-conjugateOpticalProperties:λex =494 nmλem=520 nmεmax=7.3×104Φf= 0.92τfl =5.0nsBrightness= 67.2Laser = 488 nmFilterset = FITC  ATTO700Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:575g/molATTO700Datasheet OpticalProperties:λex =700 nmλem=719nmεmax=1.25×105Φf= 0.25τfl =1.6nsBrightness= 31.3Laser =676nmFilterset =Cy®5.5  ATTO680Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:631g/molATTO680Datasheet OpticalProperties:λex =680nmλem=700 nmεmax=1.25×105Φf= 0.30τfl =1.7nsBrightness=37.5Laser =633 –676nmFilterset =Cy®5.5  ATTO655Overview:HighfluorescenceyieldHighthermalandphotostabilityExcellentozoneresistanceQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:634g/molATTO655DatasheetOpticalProperties:λex =663nmλem=684nmεmax=1.25×105Φf= 0.30τfl =1.8nsBrightness= 37.5Laser =633 –647nmFilterset =Cy®5  ATTO633Overview:HighfluorescenceyieldHighthermalandphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsStableatpH4–11Cationicdye,perchloratesaltMolarMass:652.2g/molATTO633DatasheetOpticalProperties:λex =629nmλem=657nmεmax=1.3×105Φf= 0.64τfl =3.2nsBrightness=83.2Laser =633 nmFilterset =Cy®5  ATTO594Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:1137g/molATTO594Datasheet OpticalProperties:λex =601 nmλem=627nmεmax=1.2×105Φf= 0.85τfl =3.5nsBrightness=102Laser =594nmFilterset =TexasRed®  ATTO565Overview:HighfluorescenceyieldHighthermalandphotostabilityGoodsolubilityinpolarsolventsExcellentsolubilityinwaterVerylittleaggregationRhodaminedyederivativeMolarMass:611g/molATTO565Datasheet OpticalProperties:λex =563nmλem=592nmεmax=1.2×105Φf= 0.9τfl =3.4nBrightness=10Laser =532nmFilterset = TRITC  ATTO488Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:804g/mol ATTO488Datasheet  OpticalProperties:λex =501nmλem=523nmεmax=9.0×104Φf= 0.80τfl =4.1nsBrightness= 72Laser = 488 nmFilterset = FITC  ATTO390Overview:HighfluorescenceyieldLargeStokes-shift(89nm)GoodphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsCoumarinderivate,unchargedLowmolarmass:343.42g/mol ATTO390DatasheetOpticalProperties:λex =390nmλem=479nmεmax=2.4×104Φf= 0.90τfl =5.0nsBrightness= 21.6Laser =365or405nm APC(Allophycocyanin)Overview:HighquantumyieldLargephycobiliprotein6chromophorespermoleculeIsolatedfromredalgaeMolecularWeight:105kDaAPCDatasheet OpticalProperties:λex =650nmλem=660nmεmax=7.0×105Φf= 0.68Brightness= 476Laser =594or633 nmFilterset =Cy®5 StreptavidinProperties:Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinityMolecularweight:53kDaFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISAStreptavidinDatasheetBiotinProperties:BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinityMolarmass:244.31g/molFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISABiotinDatasheetHRP(HorserADIshperoxidase)Properties:EnzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatargetReadilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonorsCatalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproductsChemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproductsHighturnoverrateenablesrapidgenerationofastrongsignal44kDaglycoproteinExtinctioncoefficient:100(403nm)Applications:Westernblot,immunohistochemistry,andELISAHRPDatasheetAP(AlkalinePhosphatase)Properties:Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenolsCommonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligationCatalyzestheconversionof:Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproductsFluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproductsMolecularweight:140kDaApplications:Westernblot,immunohistochemistry,andELISAAPDatasheet R-PE(R-Phycoerythrin)Overview:BroadexcitationspectrumHighquantumyieldPhotostableMemberofthephycobiliproteinfamilyIsolatedfromredalgaeExcellentsolubilityinwaterMolecularWeight:250kDaR-PEDatasheet OpticalProperties:λex =565nmλem=575nmεmax=2.0×106Φf= 0.84Brightness=1.68x103Laser = 488to561nmFilterset = TRITC PropertiesStorageBufferPBSpH7.4,50%glycerol,0.1%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationProteinGPurifiedClonalityMonoclonalCloneNumberS206B-9IsotypeIgG1SpecificityDetects~50kDa.Doesnotcross-reactwithGFAP-R416Worotherproteins(basedonKOvalidationresults).CiteThisProductStressMarqBiosciencesCat#SMC-442,RRID:AB_2701703CertificateofAnalysis1µg/mlofSMC-442wassufficientfordetectionofGFAPR416WTin20µgofratbrainlysatebycolorimetricimmunoblotanalysisusingGoatanti-mouseIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesGlialfibrillaryacidicproteinAntibody,IntermediatefilamentproteinAntibody,AstrocyteAntibody,gfaplAntibody,DKFZp459C0729Antibody,MGC139638Antibody,FLJ45472Antibody,AI836096Antibody,GFAPAntibodyResearchAreasAstrocyteMarkers,CellMarkers,CellStructure,GlialMarkers,NeuroscienceCellularLocalizationCytoplasmAccessionNumberNP_001124491.1GeneID2670SwissProtP14136ScientificBackgroundThe50kDatypeIIIintermediatefilamentproteinglialfibrillaryacidicprotein(GFAP)isamajorstructuralcomponentofastrocytes.GFAPassociateswiththecalciumbindingproteinannexinII-p2andS-100.AssociationwiththeseproteinstogetherwithphosphorylationregulatesGFAPpolymerization.Astroycytesrespondtobraininjurybyproliferatin(astrogliosis),andoneofthefirsteventstooccurduringastrocyteproiliferationisincreasedGFAPexpression.Interestingly,antibodiestoGFAPhavebeendetectedinindividualswithdementia.References1.VelascoM.E.,etal.(1980)Cancer.45:484.2.BonninJ.M.,etal.(1984)ActaNeuropathology.62:185.3.LeeVM-Y.,etal.(1984)J.NeuRochem.42:25-32(1984).4.TrojanowskiJQetal.(1986)J.Neurochem.6(3):650-660(1986).5.SchmidtMLetal;LabInvest56:282-294(1987).6.KosikKSetal;Neuron1:817-825(1988).7.SchmidtMLetal;LabInvest59:460-466(1988).8.Mokuna,K,etal:JNeurosciRes23:396(1989).9.Molenaar,etal;ExpNeurology108:1-9(1990).10.TohyamaTetal;AmJPathol,142:871-882(1993).11.TohyamaTetal;AmJPathol142:883-892(1993).12.Thilenius,A.R.B.,etal;J.Immunol.162(2):643-650(1999).ProductImagesImmunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-GFAPR416WTMonoclonalAntibody,CloneS206B-9(SMC-442).Tissue:NeuroblastomacelllineSK-N-BE.Species:Human.Fixation:4%Formaldehydefor15minatRT.PrimaryAntibody:MouseAnti-GFAPR416WTMonoclonalAntibody(SMC-442)at1:100for60minatRT.SecondaryAntibody:GoatAnti-MouseATTO488at1:100for60minatRT.Counterstain:PhalloidinTexasRedF-Actinstain;DAPI(blue)nuclearstainat1:1000,1:5000for60minRT,5minRT.Localization:Cytoplasm.Magnification:60X.(A)DAPI(blue)nuclearstain(B)PhalloidinTexasRedF-Actinstain(C)GFAPR416WTAntibody(D)Composite.WesternBlotanalysisofRatBrainMembraneshowingdetectionofGFAPproteinusingMouseAnti-GFAPMonoclonalAntibody,CloneS206B-9(SMC-442).PrimaryAntibody:MouseAnti-GFAPMonoclonalAntibody(SMC-442)at1:250.ProductCitations(0)Currentlytherearenocitationsforthisproduct. ATTO633Overview:HighfluorescenceyieldHighthermalandphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsStableatpH4–11Cationicdye,perchloratesaltMolarMass:652.2g/molATTO633DatasheetOpticalProperties:λex =629nmλem=657nmεmax=1.3×105Φf= 0.64τfl =3.2nsBrightness=83.2Laser =633 nmFilterset =Cy®5