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StressMarq/Anti-GIT1 Antibody (pTyr545)/SPC-987D-ALP/100-µl

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Overview: Product Name GIT1 Antibody (pTyr545) DescriptionRabbit Anti-Human GIT1 (pTyr545) Polyclonal Species Reactivity Human Applications WB, AM Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user. Host Species Rabbit Immunogen Species Human Immunogen A phospho-specific peptide corresponding to residues surrounding Tyr545 of human GIT1 (AA542-548) Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated Properties Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal Storage Temperature -20ºC Shipping Temperature Blue Ice or 4ºC Purification Peptide Affinity Purified Clonality Polyclonal Specificity Detects 84.341 kDa. Cite This Product StressMarq Biosciences Cat# SPC-987, RRID: AB_2708165 Certificate of Analysis A 1:250 dilution of SPC-987 was sufficient for detection of GIT1 (pTyr545) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. Biological Description Alternative Names ARF GAP GIT1 Antibody, ARF GTPase activating protein GIT1 Antibody, GIT1 Antibody, Cool associated and tyrosine phosphorylated protein 1 Antibody, G protein coupled receptor kinase interactor 1 Antibody, GRK interacting protein 1 Antibody Cellular Localization Cytoplasm, Aggresome, Cytosol, Focal Adhesion, Membrane Accession Number NP_001078923 Gene ID 28964 Swiss Prot Q9Y2X7 Scientific Background G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1. Product Images Currently there are no images for this product Product Citations (0) Currently there are no citations for this product.AP (Alkaline Phosphatase)Properties:Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenolsCommonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligationCatalyzes the conversion of:Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured productsFluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent productsMolecular weight: 140 kDaApplications: Western blot, immunohistochemistry, and ELISAAP Datasheet

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