Overview:ProductNameGluN2B/NR2BAntibodyDescriptionMouseAnti-RatGluN2B/NR2BMonoclonalIgG2aSpeciesReactivityHuman,Mouse,RatApplicationsWB,IHC,ICC/IF,IP,AMAntibodyDilutionWB(1:1000),IHC(1:1000),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesMouseImmunogenSpeciesRatImmunogenFusionproteinaminoacids20-271(N-terminus)ofratNR2BConcentration1mg/mlConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated PerCP Overview:Peridinin-Chlorophyll-ProteinComplexSmallphycobiliproteinIsolatedfromredalgaeLargestokesshift(195nm)MolecularWeight:35kDaPerCPDatasheet OpticalProperties:λex =482nmλem=677nmεmax=1.96x106Laser =488nm  PE/ATTO594PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.Overview:HighfluorescenceyieldHighphotostABIlityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationPE/ATTO594DatasheetOpticalProperties:λex =535nmλem =627 nmLaser = 488to561nm  FITC(Fluorescein)Overview:ExcellentfluorescencequantumyieldHighrateofphotobleachingGoodsolubilityinwaterBroademissionspectrumpHdependentspectraMolecularformula:C20H12O5Molarmass:332.3g/molFITC-Fluorescent-conjugateOpticalProperties:λex =494 nmλem=520 nmεmax=7.3×104Φf= 0.92τfl =5.0nsBrightness= 67.2Laser = 488 nmFilterset = FITC  ATTO700Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:575g/molATTO700Datasheet OpticalProperties:λex =700 nmλem=719nmεmax=1.25×105Φf= 0.25τfl =1.6nsBrightness= 31.3Laser =676nmFilterset =Cy®5.5  ATTO680Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:631g/molATTO680Datasheet OpticalProperties:λex =680nmλem=700 nmεmax=1.25×105Φf= 0.30τfl =1.7nsBrightness=37.5Laser =633 –676nmFilterset =Cy®5.5  ATTO655Overview:HighfluorescenceyieldHighthermalandphotostabilityExcellentozoneresistanceQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:634g/molATTO655DatasheetOpticalProperties:λex =663nmλem=684nmεmax=1.25×105Φf= 0.30τfl =1.8nsBrightness= 37.5Laser =633 –647nmFilterset =Cy®5  ATTO633Overview:HighfluorescenceyieldHighthermalandphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsStableatpH4–11Cationicdye,perchloratesaltMolarMass:652.2g/molATTO633DatasheetOpticalProperties:λex =629nmλem=657nmεmax=1.3×105Φf= 0.64τfl =3.2nsBrightness=83.2Laser =633 nmFilterset =Cy®5  ATTO594Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:1137g/molATTO594Datasheet OpticalProperties:λex =601 nmλem=627nmεmax=1.2×105Φf= 0.85τfl =3.5nsBrightness=102Laser =594nmFilterset =TexasRed®  ATTO565Overview:HighfluorescenceyieldHighthermalandphotostabilityGoodsolubilityinpolarsolventsExcellentsolubilityinwaterVerylittleaggregationRhodaminedyederivativeMolarMass:611g/molATTO565Datasheet OpticalProperties:λex =563nmλem=592nmεmax=1.2×105Φf= 0.9τfl =3.4nBrightness=10Laser =532nmFilterset = TRITC  ATTO488Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:804g/mol ATTO488Datasheet  OpticalProperties:λex =501nmλem=523nmεmax=9.0×104Φf= 0.80τfl =4.1nsBrightness= 72Laser = 488 nmFilterset = FITC  ATTO390Overview:HighfluorescenceyieldLargeStokes-shift(89nm)GoodphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsCoumarinderivate,unchargedLowmolarmass:343.42g/mol ATTO390DatasheetOpticalProperties:λex =390nmλem=479nmεmax=2.4×104Φf= 0.90τfl =5.0nsBrightness= 21.6Laser =365or405nm APC(Allophycocyanin)Overview:HighquantumyieldLargephycobiliprotein6chromophorespermoleculeIsolatedfromredalgaeMolecularWeight:105kDaAPCDatasheet OpticalProperties:λex =650nmλem=660nmεmax=7.0×105Φf= 0.68Brightness= 476Laser =594or633 nmFilterset =Cy®5 StreptavidinProperties:Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinityMolecularweight:53kDaFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISAStreptavidinDatasheetBiotinProperties:BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinityMolarmass:244.31g/molFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISABiotinDatasheetHRP(HorserADIshperoxidase)Properties:EnzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatargetReadilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonorsCatalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproductsChemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproductsHighturnoverrateenablesrapidgenerationofastrongsignal44kDaglycoproteinExtinctioncoefficient:100(403nm)Applications:Westernblot,immunohistochemistry,andELISAHRPDatasheetAP(AlkalinePhosphatase)Properties:Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenolsCommonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligationCatalyzestheconversionof:Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproductsFluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproductsMolecularweight:140kDaApplications:Westernblot,immunohistochemistry,andELISAAPDatasheet R-PE(R-Phycoerythrin)Overview:BroadexcitationspectrumHighquantumyieldPhotostableMemberofthephycobiliproteinfamilyIsolatedfromredalgaeExcellentsolubilityinwaterMolecularWeight:250kDaR-PEDatasheet OpticalProperties:λex =565nmλem=575nmεmax=2.0×106Φf= 0.84Brightness=1.68x103Laser = 488to561nmFilterset = TRITC PropertiesStorageBufferPBSpH7.4,50%glycerol,0.09%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationProteinGPurifiedClonalityMonoclonalCloneNumberS59-20IsotypeIgG2aSpecificityDetects~166kDa.Nocross-reactivityagainstNR2A.CiteThisProductStressMarqBiosciencesCat#SMC-333,RRID:AB_10599356CertificateofAnalysis1µg/mlofSMC-333wassufficientfordetectionofNR2B/GluN2Bin10µgofratbrainlysatebycolorimetricimmunoblotanalysisusinggoatanti-mouseIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesGlutamatereceptorionotropic,NMDA2B,NMDAR2BAntibody,hNR3Antibody,MGC142180Antibody,glutamatereceptorsubunitepsilon-2Antibody,NR3Antibody,GluN2BAntibody,hNR3Antibody,MRD6Antibody,NMDAR2BAntibody,NR2BAntibody,Glutamate[NMDA]receptorsubunitepsilon-2Antibody,N-methylD-aspartatereceptorsubtype2BAntibodyResearchAreasGlutamateReceptors,IonChannels,Neurodegeneration,Neuroscience,NeurotransmitterReceptors,NMDAReceptorsCellularLocalizationCellJunction,Cellmembrane,Postsynapticcellmembrane,SynapseAccessionNumberNP_036706.1GeneID24410SwissProtQ00960ScientificBackgroundNR2B/GluN2Bcontainingreceptorshavebeenimplicatedinsynapticplasticity,memoryformationandpainmodulation(1).StudiessuggestthattheNR2Bsubunitofglutamatereceptorsmaybepotentialtargetsforrelievingpain;NR2Bmaybeaprobabletargetforanti-nociceptivedrugs,andmayalsobeusefulasanalgesics(2).References1.ZhuoM.(2009)MolBrain.2(1):42.GurwitzD,andWeizmanA.(2002)DrugDiscoveryToday,7(7):403-406.ProductImagesImmunohistochemistryanalysisusingMouseAnti-GluN2B/NR2BMonoclonalAntibody,CloneS59-20(SMC-333).Tissue:backskin.Species:Mouse.Fixation:Bouin’sFixativeandparaffin-embedded.PrimaryAntibody:MouseAnti-GluN2B/NR2BMonoclonalAntibody(SMC-333)at1:100for1houratRT.SecondaryAntibody:FITCGoatAnti-Mouse(green)at1:50for1houratRT.Localization:Positive(dull)suprabasalstainingintheepidermisandsomehairfolliclelocalization.WesternBlotanalysisofRatbrainmembranelysateshowingdetectionofGluN2B/NR2BproteinusingMouseAnti-GluN2B/NR2BMonoclonalAntibody,CloneS59-20(SMC-333).Load:15µgprotein.Block:1.5%BSAfor30minutesatRT.PrimaryAntibody:MouseAnti-GluN2B/NR2BMonoclonalAntibody(SMC-333)at1:1000for2hoursatRT.SecondaryAntibody:SheepAnti-MouseIgG:HRPfor1houratRT.ProductCitations(2)OtherCitationsBioMarkerAnalysiswithGratingCoupledSurfacePlasmonCoupledFluorescence.Mendoza,A.,Dias,J.A.,Zeltner,T.andLawrence,D.A.(2014)JAdvBio&Biotech.1(1):1-22.PubMedID:N/AReactivityHumanApplications:AntibodyMicroarrayBiomarkerAnalysiswithGratingCoupledSurfacePlasmonCoupledFluorescence.Mendoza,A.,Dias,J.A.,Zeltner,T.andLawrence,D.A.(2014)JAdvBio&Biotech.1(1):1-22.PubMedID:N/AReactivityMouseApplications:AntibodyMicroarray ATTO594Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:1137g/molATTO594Datasheet OpticalProperties:λex =601 nmλem=627nmεmax=1.2×105Φf= 0.85τfl =3.5nsBrightness=102Laser =594nmFilterset =TexasRed®