Overview: Product Name GLUT1 Antibody DescriptionRabbit Anti-Human GLUT1 Polyclonal Species Reactivity Human, Mouse, Rat Applications WB, IHC, ELISA Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:40000); optimal dilutions for assays should be determined by the user. Host Species Rabbit Immunogen Species Human Immunogen Synthesized peptide derived from human GLUT1. Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated Properties Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide Storage Temperature -20ºC Shipping Temperature Blue Ice or 4ºC Purification Affinity Purified Clonality Polyclonal Isotype IgG Specificity Detects ~54kDa, endogenous levels of total GLUT1 protein. Cite This Product StressMarq Biosciences Cat# SPC-1295, RRID: AB_2713115 Certificate of Analysis A 1:1000 dilution of SPC-1295 was sufficient for detection of GLUT1 in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. Biological Description Alternative Names Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) Antibody, CSE Antibody, DYT17 Antibody, DYT18 Antibody, DYT9 Antibody, EIG12 Antibody, erythrocyte/brain Antibody, Erythrocyte/hepatoma glucose transporter Antibody, facilitated glucose transporter member 1 Antibody, Glucose transporter 1 Antibody, Glucose transporter type 1 Antibody, Glucose transporter type 1, erythrocyte/brain Antibody, GLUT Antibody, GLUT-1 Antibody, GLUT1 Antibody, GLUT1DS Antibody, GLUTB Antibody, GT1 Antibody, GTG1 Antibody, Gtg3 Antibody, GTR1_HUMAN Antibody, HepG2 glucose transporter Antibody, HTLVR Antibody, Human T cell leukemia virus (I and II) receptor Antibody, MGC141895 Antibody, MGC141896 Antibody, PED Antibody, RATGTG1 Antibody, Receptor for HTLV 1 and HTLV 2 Antibody, SLC2A1 Antibody, Solute carrier family 2 (facilitated glucose transporter), member 1 Antibody, Solute carrier family 2 Antibody, Solute carrier family 2, facilitated glucose transporter member 1 Antibody Research Areas Cancer, Atherosclerosis, Cardiovascular System Cellular Localization Cell membrane, Melanosome Accession Number NP_006507.2 Gene ID 6513 Swiss Prot P11166 Scientific Background Glucose transporter 1 is a uniporter protein that in humans is encoded by the SLC2A1 gene, also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1). GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier, whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake. References 1. Mueckler M. et al. (1985) Science 229 (4717): 941–5. 2. Olson A.L. and Pessin J.E. (1996) Annual Review of Nutrition 16: 235–56. Product Images Immunohistochemistry analysis using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Tissue: Brain Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:100. The image on the right is treated with the synthesized peptide. Western blot analysis of Human Jurkat cell lysates showing detection of ~55kDa GLUT1 protein using Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295). Lane 1: Human Jurkat cells. Lane 2: Human Jurkat cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-GLUT1 Polyclonal Antibody (SPC-1295) at 1:1000. Predicted/Observed Size: ~55kDa. Product Citations (0) Currently there are no citations for this product. ATTO 700Overview:High fluorescence yieldExcellent thermal and photostabilityQuenched by electron donorsVery hydrophilicGood solubility in polar solventsZwitterionic dyeMolar Mass: 575 g/molATTO 700 Datasheet Optical Properties:λex = 700 nmλem = 719 nmεmax = 1.25×105Φf = 0.25τfl = 1.6 nsBrightness = 31.3Laser = 676 nmFilter set = Cy®5.5