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StressMarq/Anti-HA tag Antibody [HA.C5]/SMC-260D-PCP/100-µl

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SMC-260D-PCP

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Overview: Product Name HA tag Antibody DescriptionMouse Anti-HA tag Monoclonal IgG3 Species Reactivity Species Independent Applications WB, ICC/IF, IP Antibody Dilution WB (1:1000), ICC/IF (1:400), IP (1:400); optimal dilutions for assays should be determined by the user. Host Species Mouse Immunogen Species Human Immunogen A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH. Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated Properties Storage Buffer PBS, pH 7.4, 50% glyercol, 0.05% sodium azide Storage Temperature -20ºC Shipping Temperature Blue Ice or 4ºC Clonality Monoclonal Clone Number HA.C5 Isotype IgG3 Specificity HA-tag monoclonal antibody. Recognizes HA-tagged proteins overexpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells. Cite This Product StressMarq Biosciences Cat# SMC-260, RRID: AB_2699826 Certificate of Analysis A 1:1000 dilution of SMC-260 was sufficient for detection of HA Tag in 10 µg of Human Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody. Biological Description Alternative Names HA epitope tag antibody, HA1 antibody, HA2 antibody, hemagglutinin antibody, Hemagglutinin HA1 chain antibody, Hemagglutinin HA2 chain antibody Research Areas Tags and Cell Markers Scientific Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 and has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. References 1. Schembri, L. et al. (2007) Nature Methods 4 (2): 107–108. Product Images Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260). Secondary Antibody: APC Goat Anti-Mouse (red). Western Blot analysis of Human Hek293 cell lysates showing detection of HA Tag protein using Mouse Anti-HA Tag Monoclonal Antibody, Clone HA.C5 (SMC-260). Lane 1: Human 293T cells (Untransfected Control). Lane 2: Human 293T cells transfected with HA-Tag Vector.. Primary Antibody: Mouse Anti-HA Tag Monoclonal Antibody (SMC-260) at 1:1000. Product Citations (0) Currently there are no citations for this product. PerCP Overview:Peridinin-Chlorophyll-Protein ComplexSmall phycobiliproteinIsolated from red algaeLarge stokes shift (195 nm)Molecular Weight: 35 kDaPerCP Datasheet Optical Properties:λex = 482 nmλem = 677 nmεmax = 1.96 x 106Laser = 488 nm

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