Overview: Product Name HO-1 (Rat) Antibody DescriptionRabbit Anti-Rat HO-1 (Rat) Polyclonal Species Reactivity Mouse, Rat Applications WB Antibody Dilution WB (1:1000); optimal dilutions for assays should be determined by the user. Host Species Rabbit Immunogen Species Rat Immunogen Rat HO-1 without the transmembrane domain (cloned residues 1-262) Concentration 1 mg/ml Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated Properties Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide Storage Temperature -20ºC Shipping Temperature Blue Ice or 4ºC Purification Protein A purified Clonality Polyclonal Specificity Detects 32kDa. Does not cross-react with HO-2. Cite This Product StressMarq Biosciences Cat# SPC-207, RRID: AB_2704235 Certificate of Analysis 1 µg/ml of SPC-207 was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. Biological Description Alternative Names Heme oxygenase 1 Antibody, Hemox Antibody, HMOX1 Antibody, HO1 Antibody, HO 1 Antibody, HSP32 Antibody Research Areas Cancer, Oxidative Stress Cellular Localization Endoplasmic Reticulum, Microsome Accession Number NP_036712.1 Gene ID 24451 Swiss Prot P06762 Scientific Background Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4). References 1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135. Product Images Currently there are no images for this product Product Citations (1) Western Blot Protective effects of heme oxygenase-1 against MPP(+)-induced cytotoxicity in PC-12 cells. Bae, J.W., Kim, M.J., Jang, C.G. and Lee, S.Y. (2010) Neurol Sci. 31 (3): 307-313. PubMed ID: 20127499 Reactivity Rat Applications: Western Blot ATTO 655Overview:High fluorescence yieldHigh thermal and photostabilityExcellent ozone resistanceQuenched by electron donorsVery hydrophilicGood solubility in polar solventsZwitterionic dyeMolar Mass: 634 g/molATTO 655 DatasheetOptical Properties:λex = 663 nmλem = 684 nmεmax = 1.25×105Φf = 0.30τfl = 1.8 nsBrightness = 37.5Laser = 633 – 647 nmFilter set = Cy®5