Overview:ProductNameHO-1(Rat)AntibodyDescriptionRabbitAnti-RatHO-1(Rat)PolyclonalSpeciesReactivityMouse,RatApplicationsWBAntibodyDilutionWB(1:1000);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesRabbitImmunogenSpeciesRatImmunogenRatHO-1withoutthetransmembranedomain(clonedresidues1-262)Concentration1mg/mlConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,UnconjugatedPropertiesStorageBufferPBSpH7.4,50%glycerol,0.09%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationProteinApurifiedClonalityPolyclonalSpecificityDetects32kDa.Doesnotcross-reactwithHO-2.CiteThisProductStressMarqBiosciencesCat#SPC-207,RRID:AB_2704235CertificateofAnalysis1µg/mlofSPC-207wassufficientfordetectionofHO-1in10µgofmixedhumancelllinelysatebycolorimetricimmunoblotanalysisusingGoatanti-rabbitIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesHemeoxygenase1Antibody,HemoxAntibody,HMOX1Antibody,HO1Antibody,HO1Antibody,HSP32AntibodyResearchAreasCancer,OxidativeStressCellularLocalizationEndoplasmicReticulum,MicrosomeAccessionNumberNP_036712.1GeneID24451SwissProtP06762ScientificBackgroundHeme-oxygenaseisaubiquitousenzymethatcatalyzestheinitialandrate-limitingstepsinhemecatabolismyieldingequimolaramountsofbiliverdin,ironandcarbonmonoxide.Biliverdinissubsequentlyconvertedtobilirubinandthefreeironissequesteredtoferritin(1).Theseproductshaveimportantphysiologicaleffectsascarbonmonoxideisapotentvasodilator;biliverdinandbilirubinarepotentantioxidants;andthefreeironincreasesoxidativestressandregulatestheexpressionofmanymRNAs(2).Therearethreeisoformsofheme-oxygenase,HO-1,HO-2andHO-3;howeverHO-1andHO-2arethemajorisoformsastheybothhavebeenidentifiedinmammals(3).HO-1,alsoknownasheatshockprotein32,isaninducIBLeisoformactivatedbymostoxidativestressinducers,cytokines,inflammatoryagentsandheatshock.HO-2isaconstitutiveisoformwhichisexpressedunderhomeostaticconditions.HO-1isalsoconsideredtobeacytoprotectivefactorinthatfreehemeishighlyreactiveandcytotoxic,andsecondly,carbonmonoxideisamediatorinhibitingtheinflammatoryprocessandbilirubinisascavengerforreactiveoxygen,bothofwhicharetheendproductsofhemecatalyzation(4).IthasalsobeenshownthatHO-1deficiencymaycausereducedstressdefense,apro-inflammatorytendency(5),susceptibilitytoatheroscleroticlesionformation(6),endothelialcellinjury,andgrowthretardation(7).Up-regulationofHO-1isthereforesaidtobeoneofthemajordefensemechanismsofoxidativestress(4).References1.FrohM.etal.(2007)WorldJ.Gastroentereol13(25):3478-86.2.ElbirtK.K.andBonkovskyH.L.(1999)ProcAssocAmPhysicians111(5):348-47.3.MainesM.D.,TrakshelG.M.,andKuttyR.K.(1986)JBiolChem261:411–419.4.BrydunA.,etal.(2007)HypertensRes30(4):341-8.5.PossK.D.andTonegawaS.(1997).ProcNatlAcadSciUSA.94:10925–10930.6.YetS.F.,etal.(2003)FASEBJ.17:1759–1761.7.YachieA.,etal.(1999)JClinInvest.103:129–135.ProductImagesCurrentlytherearenoimagesforthisproductProductCitations(1)WesternBlotProtectiveeffectsofhemeoxygenase-1againstMPP(+)-inducedcytotoxicityinPC-12cells.Bae,J.W.,Kim,M.J.,Jang,C.G.andLee,S.Y.(2010)NeurolSci.31(3):307-313.PubMedID:20127499ReactivityRatApplications:WesternBlotHRP(HorserADIshperoxidase)Properties:EnzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatargetReadilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonorsCatalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproductsChemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproductsHighturnoverrateenablesrapidgenerationofastrongsignal44kDaglycoproteinExtinctioncoefficient:100(403nm)Applications:Westernblot,immunohistochemistry,andELISAHRPDatasheet