Overview:ProductNameHSP27Antibody(pSer78)DescriptionRabbitAnti-HumanHSP27(pSer78)PolyclonalSpeciesReactivityHumanApplicationsWB,IHC,ICC/IFAntibodyDilutionWB(1:1000),IHC(1:100),ICC/IF(1:200);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesRabbitImmunogenSpeciesHumanImmunogenSynthesizedphosphopeptidederivedfromhumanHSP27aroundthephosphorylationsiteofserine78(A-L-SP-R-Q).ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,UnconjugatedPropertiesStorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationAffinityPurifiedClonalityPolyclonalIsotypeIgGSpecificityDetectsendogenouslevelsofHSP27onlywhenphosphorylatedatserine78.CiteThisProductStressMarqBiosciencesCat#SPC-1265,RRID:AB_2712575CertificateofAnalysisA1:1000dilutionofSPC-1265wassufficientfordetectionofHsp27in10µgofHeLacelllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNames28kDaheatshockproteinAntibody,CMT2FAntibody,HSP25Antibody,HSP27Antibody,HSP28Antibody,HSPB1Antibody,SRP27AntibodyResearchAreasCancer,HeatShock,Atherosclerosis,CardiovascularSystem,ChaperoneProteins,Chaperones,Heart,ProteinTrafficking,TraffickingCellularLocalizationCytoplasm,NucleusAccessionNumberNP_001531.1GeneID3315SwissProtP04792ScientificBackgroundHSP27sbelongtoanabundantandubiquitousfamilyofsmallheatshockproteins(sHSP).ItisanimportantHSPfoundinbothnormalhumancellsandcancercells.ThebasicstructureofmostsHSPsisahomologousandhighlyconservedaminoacidsequence,withanα-crystallindomainattheC-terminusandtheWD/EPFdomainatthelessconservedN-terminus.ThisN-terminusisessentialforthedevelopmentofhighmolecularoligomers(1,2).HSP27-oligomersconsistofstabledimersformedbyasmanyas8-40HSP27proteinmonomers(3).Theoligomerizationstatusisconnectedwiththechaperoneactivity:aggregatesoflargeoligomershavehighchaperoneactivity,whereasdimershavenochaperoneactivity(4).HSP27islocalizedtothecytoplasmofunstressedcellsbutcanredistributetothenucleusinresponsetostress,whereitmayfunctiontostABIlizeDNAand/orthenuclearmembrane.Otherfunctionsincludechaperoneactivity(asmentionedabove),Thermotoleranceinvivo,inhibitionofapoptosis,andsignaltransduction.Specifically,invitro,itactsasanATP-independentchaperonebyinhibitingproteinaggregationandbystabilizingpartiallydenaturedproteins,whichensuresrefoldingoftheHSP70complex.HSP27isalsoinvolvedintheapoptoticsignalingpathwaybecauseitinterfereswiththeactivationofcytochromec/Apaf-1/dATPcomplex,therebyinhibitingtheactivationofprocaspase-9.ItisalsohypothesizedthatHSP27mayservesomeroleincross-bridgeformationbetweenactinandmyosin(5).Andfinally,HSP27isalsothoughttobeinvolvedintheprocessofcelldifferentiation.Theup-regulationofHSP27correlateswiththerateofphosphorylationandwithanincreaseoflargeoligomers.ItispossIBLethatHSP27mayplayacrucialroleinterminationofgrowth(6).LookingformoreinformationonHSP27?VisitournewHSP27ScientificResourceGuideathttp://www.HSP27.com.References1.KimK.K.,KimR.,andKim,S.(1998)Nature394(6693):595-599.2.VanMontfortR.,SlingsbyC.,andVierlingE.(2001)AddvProteinChem.59:105-56.3.EhrnspergerM.,GraberS.,GaestelM.andBuchnerJ.(1997)EMBOJ.16:221-229.4.CioccaD.R.,OesterreichS.,ChamnessG.C.,McGuireW.L.,andFuguaS.A.(1993)JNatlCancerInst.85(19):1558-70.5.SartoC.,BinnzP.A.,andMocarelliP.(2000)Electrophoresis.21(6):1218-26.6.ArrigoA.P.(2005)JCellBiochem.94(2):241-6.ProductImagesImmunohistochemistryanalysisusingRabbitAnti-Hsp27PolyclonalAntibody(SPC-1265).Tissue:BreastCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-Hsp27PolyclonalAntibody(SPC-1265)at1:1000.Theimageontherightistreatedwiththesynthesizedpeptide.WesternblotanalysisofHumanHeLacelllysatesshowingdetectionof~27kDaHsp27proteinusingRabbitAnti-Hsp27PolyclonalAntibody(SPC-1265).Lane1andLane2:HumanHeLa,Hsp27Antibody(Ser78).Lane3andLane4:HumanHeLa,Hsp27Antibody(pSer78).PrimaryAntibody:RabbitAnti-Hsp27PolyclonalAntibody(SPC-1265)at1:1000.Predicted/ObservedSize:~27kDa.ProductCitations(0)Currentlytherearenocitationsforthisproduct. ATTO488Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:804g/mol ATTO488Datasheet  OpticalProperties:λex =501nmλem=523nmεmax=9.0×104Φf= 0.80τfl =4.1nsBrightness= 72Laser = 488 nmFilterset = FITC