Overview:ProductNameHSP40,YDJ1AntibodyDescriptionMouseAnti-YeastHSP40,YDJ1MonoclonalIgG1KappaSpeciesReactivityYeastApplicationsWB,IP,ELISAAntibodyDilutionWB(1:2000);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesMouseImmunogenSpeciesYeastImmunogenFulllengthproteinHSP40(YDJ1)Concentration1mg/mlConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated PerCP Overview:Peridinin-Chlorophyll-ProteinComplexSmallphycobiliproteinIsolatedfromredalgaeLargestokesshift(195nm)MolecularWeight:35kDaPerCPDatasheet OpticalProperties:λex =482nmλem=677nmεmax=1.96x106Laser =488nm  PE/ATTO594PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.Overview:HighfluorescenceyieldHighphotostABIlityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationPE/ATTO594DatasheetOpticalProperties:λex =535nmλem =627 nmLaser = 488to561nm  FITC(Fluorescein)Overview:ExcellentfluorescencequantumyieldHighrateofphotobleachingGoodsolubilityinwaterBroademissionspectrumpHdependentspectraMolecularformula:C20H12O5Molarmass:332.3g/molFITC-Fluorescent-conjugateOpticalProperties:λex =494 nmλem=520 nmεmax=7.3×104Φf= 0.92τfl =5.0nsBrightness= 67.2Laser = 488 nmFilterset = FITC  ATTO700Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:575g/molATTO700Datasheet OpticalProperties:λex =700 nmλem=719nmεmax=1.25×105Φf= 0.25τfl =1.6nsBrightness= 31.3Laser =676nmFilterset =Cy®5.5  ATTO680Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:631g/molATTO680Datasheet OpticalProperties:λex =680nmλem=700 nmεmax=1.25×105Φf= 0.30τfl =1.7nsBrightness=37.5Laser =633 –676nmFilterset =Cy®5.5  ATTO655Overview:HighfluorescenceyieldHighthermalandphotostabilityExcellentozoneresistanceQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:634g/molATTO655DatasheetOpticalProperties:λex =663nmλem=684nmεmax=1.25×105Φf= 0.30τfl =1.8nsBrightness= 37.5Laser =633 –647nmFilterset =Cy®5  ATTO633Overview:HighfluorescenceyieldHighthermalandphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsStableatpH4–11Cationicdye,perchloratesaltMolarMass:652.2g/molATTO633DatasheetOpticalProperties:λex =629nmλem=657nmεmax=1.3×105Φf= 0.64τfl =3.2nsBrightness=83.2Laser =633 nmFilterset =Cy®5  ATTO594Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:1137g/molATTO594Datasheet OpticalProperties:λex =601 nmλem=627nmεmax=1.2×105Φf= 0.85τfl =3.5nsBrightness=102Laser =594nmFilterset =TexasRed®  ATTO565Overview:HighfluorescenceyieldHighthermalandphotostabilityGoodsolubilityinpolarsolventsExcellentsolubilityinwaterVerylittleaggregationRhodaminedyederivativeMolarMass:611g/molATTO565Datasheet OpticalProperties:λex =563nmλem=592nmεmax=1.2×105Φf= 0.9τfl =3.4nBrightness=10Laser =532nmFilterset = TRITC  ATTO488Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:804g/mol ATTO488Datasheet  OpticalProperties:λex =501nmλem=523nmεmax=9.0×104Φf= 0.80τfl =4.1nsBrightness= 72Laser = 488 nmFilterset = FITC  ATTO390Overview:HighfluorescenceyieldLargeStokes-shift(89nm)GoodphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsCoumarinderivate,unchargedLowmolarmass:343.42g/mol ATTO390DatasheetOpticalProperties:λex =390nmλem=479nmεmax=2.4×104Φf= 0.90τfl =5.0nsBrightness= 21.6Laser =365or405nm APC(Allophycocyanin)Overview:HighquantumyieldLargephycobiliprotein6chromophorespermoleculeIsolatedfromredalgaeMolecularWeight:105kDaAPCDatasheet OpticalProperties:λex =650nmλem=660nmεmax=7.0×105Φf= 0.68Brightness= 476Laser =594or633 nmFilterset =Cy®5 StreptavidinProperties:Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinityMolecularweight:53kDaFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISAStreptavidinDatasheetBiotinProperties:BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinityMolarmass:244.31g/molFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISABiotinDatasheetHRP(HorserADIshperoxidase)Properties:EnzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatargetReadilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonorsCatalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproductsChemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproductsHighturnoverrateenablesrapidgenerationofastrongsignal44kDaglycoproteinExtinctioncoefficient:100(403nm)Applications:Westernblot,immunohistochemistry,andELISAHRPDatasheetAP(AlkalinePhosphatase)Properties:Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenolsCommonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligationCatalyzestheconversionof:Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproductsFluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproductsMolecularweight:140kDaApplications:Westernblot,immunohistochemistry,andELISAAPDatasheet R-PE(R-Phycoerythrin)Overview:BroadexcitationspectrumHighquantumyieldPhotostableMemberofthephycobiliproteinfamilyIsolatedfromredalgaeExcellentsolubilityinwaterMolecularWeight:250kDaR-PEDatasheet OpticalProperties:λex =565nmλem=575nmεmax=2.0×106Φf= 0.84Brightness=1.68x103Laser = 488to561nmFilterset = TRITC PropertiesStorageBuffer50%glycerol,0.09%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationProteinGPurifiedClonalityMonoclonalCloneNumber2A7.H6IsotypeIgG1KappaSpecificityDetects~40kDa.Yeastspecific.DoesnotcrossreactwithHuman,MouseorRat.CiteThisProductStressMarqBiosciencesCat#SMC-166,RRID:AB_2094596CertificateofAnalysis0.5µg/mlofSMC-166wassufficientfordetectionof50ngYDJ1bycolorimetricimmunoblotanalysisusingGoatanti-mouseIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesDnaJ(HSP40)homologsubfmailyBmember1antibody,DNAJ1antibody,DNAJB1antibody,DnaJhomologsubfamilyBmember1antibody,DnaJproteinhomolog1antibody,DNAJ1antibody,DNAJB1antibody,DNAJB1antibody,DNAJB1proteinantibody,DNJB1_HUMANantibody,HDJ1antibody,HDJ-1antibody,HDJ1antibody,Heatshock40kDaprotein1antibody,Heatshock40kDprotein1antibody,Heatshockprotein40antibody,HSP40antibody,HSP40antibody,HSPF1antibody,HSPF1antibody,HumanDnaJprotein1antibody,Radialspoke16homologBantibody,RSPH16Bantibody,Sis1antibodyResearchAreasCancer,HeatShockCellularLocalizationCytoplasm,NucleusAccessionNumberNP_014335.1GeneID855661SwissProtP25491ScientificBackgroundHumanHSP40/DnaJproteinscomprisealargeproteinfamily,membersofwhichfeaturetheJdomain(namedafterthebacterialDnaJprotein)(1).TheJ-domainspansthefirst75N-terminalaminoacidsandisseparatedfromtheC-terminalbyaglycine/phenylalanine-richdomain(2).MembersoftheHSP40/DnaJfamilyplaydiverserolesinmanycellularprocesses,suchasfolding,translocation,degradationandassemblyofmulti-proteincomplexes.Inparticular,Hdj1,thefirsthumanHSP40/DnaJproteinidentified,playsanimportantroleinproteintranslationandfolding,aswellasintheregulationofHSP70function(3).HSP40stimulatestheATPaseactivityofHSP70whichinturncausesconformationalchangesoftheunfoldedproteins(4,5).TheHSP40-HSP70-unfoldedproteincomplexfurtherbindstoco-chaperonesHip,HopandHSP90whichleadstoproteinfolding,orcomponentsofproteindegradationmachineryCHIPandBAG-1(6).SomestudieshaveshownthatthedifferencebetweenHDJ1andtype1DNAJproteinsincludingHDJ2andyeastYdjIistheresultofthepossessionofazincfingerdomainbythelatter,whichhelpsinthefunctionofproteinfolding.(7,8).References1.CheethamM.E.andCaplanA.J.(1998)CellStressChaperones3:28–36.2.FanC.Y.,etal.(2003)CellStressChaperones8:309–316.3.SohnS.Y.,KimS.B.,KimJ.,andAhnB.Y.(2006)JGenVirol.87(7):1883-91.4.LiberekK.etal.(1991)Proc.Natl.Acad.Sci.USA88:2874–2878.5.CyrD.M.,etal.(1992)JBiolChem.267:20927–20931.6.HöhfeldJ.,etal.(2001)EMBORep.2:885–890.7.TerdaK.,etal.(1997)JCellBiol.139:1089-1095.8.LuZ.andCyrD.M.(1998)JBiolChem.273:27824-27830.ProductImagesWesternBlotanalysisofYeastrecombinantcelllysateshowingdetectionofHsp40proteinusingMouseAnti-Hsp40MonoclonalAntibody,Clone2A7.H6(SMC-166).Load:15µgprotein.Block:1.5%BSAfor30minutesatRT.PrimaryAntibody:MouseAnti-Hsp40MonoclonalAntibody(SMC-166)at1:1000for2hoursatRT.SecondaryAntibody:SheepAnti-MouseIgG:HRPfor1houratRT.ProductCitations(0)Currentlytherearenocitationsforthisproduct.