Overview:ProductNameHSP60AntibodyDescriptionMouseAnti-HumanHSP60MonoclonalIgG1SpeciesReactivityDog,Human,Monkey,Mouse,Rat,Africanclawedfrog(Xenopuslaevis),Bovine,Chicken,FruitFly(Drosophilamelanogaster),GuineaPig(Caviaporcellus),Hamster,Pig,Plant,Rabbit,Sheep,SoybeanApplicationsWB,IHC,IP,ELISA,FCMAntibodyDilutionWB(1:20000),IHC(1:100),ICC/IF(1:100),IP(1:200);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesMouseImmunogenSpeciesHumanImmunogenRecombinanthumanHSP60Concentration1mg/mlConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated PerCP Overview:Peridinin-Chlorophyll-ProteinComplexSmallphycobiliproteinIsolatedfromredalgaeLargestokesshift(195nm)MolecularWeight:35kDaPerCPDatasheet OpticalProperties:λex =482nmλem=677nmεmax=1.96x106Laser =488nm  PE/ATTO594PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.Overview:HighfluorescenceyieldHighphotostABIlityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationPE/ATTO594DatasheetOpticalProperties:λex =535nmλem =627 nmLaser = 488to561nm  FITC(Fluorescein)Overview:ExcellentfluorescencequantumyieldHighrateofphotobleachingGoodsolubilityinwaterBroademissionspectrumpHdependentspectraMolecularformula:C20H12O5Molarmass:332.3g/molFITC-Fluorescent-conjugateOpticalProperties:λex =494 nmλem=520 nmεmax=7.3×104Φf= 0.92τfl =5.0nsBrightness= 67.2Laser = 488 nmFilterset = FITC  ATTO700Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:575g/molATTO700Datasheet OpticalProperties:λex =700 nmλem=719nmεmax=1.25×105Φf= 0.25τfl =1.6nsBrightness= 31.3Laser =676nmFilterset =Cy®5.5  ATTO680Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:631g/molATTO680Datasheet OpticalProperties:λex =680nmλem=700 nmεmax=1.25×105Φf= 0.30τfl =1.7nsBrightness=37.5Laser =633 –676nmFilterset =Cy®5.5  ATTO655Overview:HighfluorescenceyieldHighthermalandphotostabilityExcellentozoneresistanceQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:634g/molATTO655DatasheetOpticalProperties:λex =663nmλem=684nmεmax=1.25×105Φf= 0.30τfl =1.8nsBrightness= 37.5Laser =633 –647nmFilterset =Cy®5  ATTO633Overview:HighfluorescenceyieldHighthermalandphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsStableatpH4–11Cationicdye,perchloratesaltMolarMass:652.2g/molATTO633DatasheetOpticalProperties:λex =629nmλem=657nmεmax=1.3×105Φf= 0.64τfl =3.2nsBrightness=83.2Laser =633 nmFilterset =Cy®5  ATTO594Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:1137g/molATTO594Datasheet OpticalProperties:λex =601 nmλem=627nmεmax=1.2×105Φf= 0.85τfl =3.5nsBrightness=102Laser =594nmFilterset =TexasRed®  ATTO565Overview:HighfluorescenceyieldHighthermalandphotostabilityGoodsolubilityinpolarsolventsExcellentsolubilityinwaterVerylittleaggregationRhodaminedyederivativeMolarMass:611g/molATTO565Datasheet OpticalProperties:λex =563nmλem=592nmεmax=1.2×105Φf= 0.9τfl =3.4nBrightness=10Laser =532nmFilterset = TRITC  ATTO488Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:804g/mol ATTO488Datasheet  OpticalProperties:λex =501nmλem=523nmεmax=9.0×104Φf= 0.80τfl =4.1nsBrightness= 72Laser = 488 nmFilterset = FITC  ATTO390Overview:HighfluorescenceyieldLargeStokes-shift(89nm)GoodphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsCoumarinderivate,unchargedLowmolarmass:343.42g/mol ATTO390DatasheetOpticalProperties:λex =390nmλem=479nmεmax=2.4×104Φf= 0.90τfl =5.0nsBrightness= 21.6Laser =365or405nm APC(Allophycocyanin)Overview:HighquantumyieldLargephycobiliprotein6chromophorespermoleculeIsolatedfromredalgaeMolecularWeight:105kDaAPCDatasheet OpticalProperties:λex =650nmλem=660nmεmax=7.0×105Φf= 0.68Brightness= 476Laser =594or633 nmFilterset =Cy®5 StreptavidinProperties:Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinityMolecularweight:53kDaFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISAStreptavidinDatasheetBiotinProperties:BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinityMolarmass:244.31g/molFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISABiotinDatasheetHRP(HorserADIshperoxidase)Properties:EnzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatargetReadilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonorsCatalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproductsChemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproductsHighturnoverrateenablesrapidgenerationofastrongsignal44kDaglycoproteinExtinctioncoefficient:100(403nm)Applications:Westernblot,immunohistochemistry,andELISAHRPDatasheetAP(AlkalinePhosphatase)Properties:Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenolsCommonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligationCatalyzestheconversionof:Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproductsFluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproductsMolecularweight:140kDaApplications:Westernblot,immunohistochemistry,andELISAAPDatasheet R-PE(R-Phycoerythrin)Overview:BroadexcitationspectrumHighquantumyieldPhotostableMemberofthephycobiliproteinfamilyIsolatedfromredalgaeExcellentsolubilityinwaterMolecularWeight:250kDaR-PEDatasheet OpticalProperties:λex =565nmλem=575nmεmax=2.0×106Φf= 0.84Brightness=1.68x103Laser = 488to561nmFilterset = TRITC PropertiesStorageBufferPBS,50%glycerol,0.09%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationProteinGPurifiedClonalityMonoclonalCloneNumberLK1IsotypeIgG1SpecificityDetects~60kDa.CiteThisProductStressMarqBiosciencesCat#SMC-110,RRID:AB_2121277CertificateofAnalysis0.05µg/mlofSMC-110wassufficientfordetectionofHSP60in20µgofheatshockedHeLacelllysatebycolorimetricimmunoblotanalysisusinggoatanti-mouseIgGasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesCPN60Antibody,GROELAntibody,HLD4Antibody,HSP60Antibody,HSP65Antibody,HSPD1Antibody,HuCHA60Antibody,SPG13AntibodyResearchAreasCancer,HeatShockCellularLocalizationMitochondrion,MitochondrionMatrixAccessionNumberNP_002147.2GeneID3329SwissProtP10809ScientificBackgroundInbothprokaryoticandeukaryoticcells,themisfoldingandaggregationofproteinsduringbiogenesisandunderconditionsofcellularstressarepreventedbymolecularchaperones.MembersoftheHSP60familyofheatshockproteinsaresomeofthebestcharacterizedchaperones.HSP60,alsoknownasCpn60orGroEl,isanabundantproteinsynthesizedconstitutivelyinthecellthatisinducedtoahigherconcentrationafterbriefcellshock.Itispresentinmanyspeciesandexhibitsaremarkablesequencehomologyamongvariouscounterpartsinbacteria,plants,andmammalswithmorethanhalfoftheresiduesidenticalbetweenbacterialandmammalianHSP60(1-3).WhereasmammalianHSP60islocalizedwithinthemitochondria,plantHSP60,orotherwiseknownasRubisco-bindingprotein,islocatedinplantchloroplasts.IthasbeenindicatedthattheseproteinscarryoutaveryimportantbiologicalfunctionduetothefactthatHSP60ispresentinsomanydifferentspecies.ThecommoncharacteristicsoftheHSP60sfromthedivergentspeciesarei)highabundance,ii)inductionwithenvironmentalstresssuchasheatshock,iii)homo-oligomericstructuresofeither7or14subunitswhichreversIBLydissociateinthepresenceofMg2+andATP,iv)ATPaseactivityandv)aroleinfoldingandassemblyofoligomericproteinstructures(4).Thesesimilaritiesaresupportedbyrecentstudieswherethesingle-ringhumanmitochondrialhomolog,HSP60withitsco-chaperonin,HSP10wereexpressedinaE.colistrain,engineeredsothatthegroEoperonisunderstrictregulatorycontrol.ThisstudyhasdemonstratedthatexpressionofHSP60-HSP10wasabletocarryoutallessentialinvivofunctionsofGroELanditsco-chaperonin,GroES(5).AnotherimportantfunctionofHSP60andHSP10istheirprotectivefunctionsagainstinfectionandcellularstress.HSP60hashoweverbeenlinkedtoanumberofautoimmunediseases,aswellasAlzheimer"s,coronaryarterydiseases,MS,anddiabetes(6-9).References1.Hartl,F.U.(1996)Nature381:571-579.2.Bukau,B.andHorwich,A.L.(1998)Cell92:351-366.3.Hartl,F.U.andHayer-Hartl,M.(2002)Science295:1852-1858.4.Jindal,S.,etal.(1989)MolecularandCellularBiology9:2279-2283.5.LaVerda,D.,etal(1999)InfectDis.Obstet.Gynecol.7:64-71.6.Itoh,H.etal.(2002)Eur.J.Biochem.269:5931-5938.7.Gupta,S.andKnowlton,A.A.J.CellMolMed.9:51-58.8.Deocaris,C.C.etal.(2006)CellStressChaperones11:116-128.9.Lai,H.C.etal.(2007)Am.J.Physiol.Endocrinol.Metab.292:E292-E297.10.Gao,Y.L.,etal(1995)J.ofImmunology154:3548-3556.11.Neuer,A.,etal(1997)EuropeanSocietyforHumanReproductionandEmbryology12(5):925-929.12.Bason,C.,etal(2003)Lancet362(9400):1971-1977.ProductImagesImmunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-Hsp60MonoclonalAntibody,CloneLK-1(SMC-110).Tissue:HaCaTcells.Species:Human.Fixation:Cold100%methanolat-20°Cfor10minutes.PrimaryAntibody:MouseAnti-Hsp60MonoclonalAntibody(SMC-110)at1:100for1houratRT.SecondaryAntibody:FITCGoatAnti-Mouse(green)at1:50for1houratRT.Localization:CytoplasmicStaining.ImmunohistochemistryanalysisusingMouseAnti-Hsp60MonoclonalAntibody,CloneLK-1(SMC-110).Tissue:coloncarcinoma.Species:Human.Fixation:Formalin.PrimaryAntibody:MouseAnti-Hsp60MonoclonalAntibody(SMC-110)at1:100000for12hoursat4°C.SecondaryAntibody:BiotinGoatAnti-Mouseat1:2000for1houratRT.Counterstain:MayerHematoxylin(purple/blue)nuclearstainat200µlfor2minutesatRT.Localization:Inflammatorycells.Magnification:40x.WesternBlotanalysisofHumanCelllinelysatesshowingdetectionofHsp60proteinusingMouseAnti-Hsp60MonoclonalAntibody,CloneLK-1(SMC-110).Load:15µgprotein.Block:1.5%BSAfor30minutesatRT.PrimaryAntibody:MouseAnti-Hsp60MonoclonalAntibody(SMC-110)at1:1000for2hoursatRT.SecondaryAntibody:SheepAnti-MouseIgG:HRPfor1houratRT.ImmunohistochemistryanalysisusingMouseAnti-Hsp60MonoclonalAntibody,CloneLK-1(SMC-110).Tissue:backskin.Species:Mouse.Fixation:Bouin’sFixativeandparaffin-embedded.PrimaryAntibody:MouseAnti-Hsp60MonoclonalAntibody(SMC-110)at1:100for1houratRT.SecondaryAntibody:FITCGoatAnti-Mouse(green)at1:50for1houratRT.Localization:Epidermis.Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-Hsp60MonoclonalAntibody,CloneLK1,(SMC-110).Tissue:skinFibroblasts.Species:Human.Fixation:Cold100%methanolfor30minutesat-20°C.PrimaryAntibody:MouseAnti-Hsp60MonoclonalAntibody(SMC-110)at1:1000for1houratRT.SecondaryAntibody:DAKOLSAB2streptavidin-peroxidasesystem.Counterstain:MayerHematoxylin(purple/blue)nuclearstain.Courtesyof:ValentinadiFelice,UniversityofPalermo,Italy.ProductCitations(5)WesternBlotTheroleofcaseinolyticmitochondrialmatrixpeptidaseproteolyticsubunit(CLPP)inregulationofmitochondrialribosomebiogenesisinmammals.Maiti,P.(2015)UniversityofCologne.PhDDissertation.PubMedID:N/AReactivityMouseApplications:WesternBlotTissue-SpecificLossofDARS2ActivatesStressResponsesIndependentlyofRespiratoryChainDeficiencyintheHeart.Dogan,S.A.etal.(2014)CellMetabolism.19(3):458-469.PubMedID:24606902ReactivityMouseApplications:WesternBlotTissue-specificdefenseandThermo-adaptivemechanismsofsoybeanseedlingsunderheatstressrevealedbyproteomicapproach.Ahsan,N.,Donnart,T.,Nouri,M.Z.,andKomatsu,S.(2010)JProteomeRes.9(8):4189-4204.PubMedID:20540562ReactivitySoybeanApplications:WesternBlotComparativeanalysesoftheproteomesofleavesandflowersatvariousstagesofdevelopmentrevealorgan-specificfunctionaldifferentiationofproteinsinsoybean.Ahgib,N.andKomatsu,S.(2009)Proteomes.9(21):4889-4907.PubMedID:19862761ReactivitySoybeanApplications:WesternBlotOtherCitationsPARLmediatesSmacproteolyticmaturationinmitochondriatopromoteapoptosis.Saita,S.etal.(2017)NatCellBiol.19(4):318-328.PubMedID:28288130ReactivityHumanApplications:WesternBlot PerCP Overview:Peridinin-Chlorophyll-ProteinComplexSmallphycobiliproteinIsolatedfromredalgaeLargestokesshift(195nm)MolecularWeight:35kDaPerCPDatasheet OpticalProperties:λex =482nmλem=677nmεmax=1.96x106Laser =488nm