Overview:ProductNameHSP90alphaAntibodyDescriptionMouseAnti-HumanHSP90alphaMonoclonalIgG2aSpeciesReactivityHuman,Mouse,RatApplicationsWB,IHC,ELISAAntibodyDilutionWB(1:1000),IHC(1:5000);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesMouseImmunogenSpeciesHumanImmunogenRecombinanthumanHSP90alpha;Specificitymappedtoaminoacids604-731Concentration1mg/mlConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated PerCP Overview:Peridinin-Chlorophyll-ProteinComplexSmallphycobiliproteinIsolatedfromredalgaeLargestokesshift(195nm)MolecularWeight:35kDaPerCPDatasheet OpticalProperties:λex =482nmλem=677nmεmax=1.96x106Laser =488nm  PE/ATTO594PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.Overview:HighfluorescenceyieldHighphotostABIlityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationPE/ATTO594DatasheetOpticalProperties:λex =535nmλem =627 nmLaser = 488to561nm  FITC(Fluorescein)Overview:ExcellentfluorescencequantumyieldHighrateofphotobleachingGoodsolubilityinwaterBroademissionspectrumpHdependentspectraMolecularformula:C20H12O5Molarmass:332.3g/molFITC-Fluorescent-conjugateOpticalProperties:λex =494 nmλem=520 nmεmax=7.3×104Φf= 0.92τfl =5.0nsBrightness= 67.2Laser = 488 nmFilterset = FITC  ATTO700Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:575g/molATTO700Datasheet OpticalProperties:λex =700 nmλem=719nmεmax=1.25×105Φf= 0.25τfl =1.6nsBrightness= 31.3Laser =676nmFilterset =Cy®5.5  ATTO680Overview:HighfluorescenceyieldExcellentthermalandphotostabilityQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:631g/molATTO680Datasheet OpticalProperties:λex =680nmλem=700 nmεmax=1.25×105Φf= 0.30τfl =1.7nsBrightness=37.5Laser =633 –676nmFilterset =Cy®5.5  ATTO655Overview:HighfluorescenceyieldHighthermalandphotostabilityExcellentozoneresistanceQuenchedbyelectrondonorsVeryhydrophilicGoodsolubilityinpolarsolventsZwitterionicdyeMolarMass:634g/molATTO655DatasheetOpticalProperties:λex =663nmλem=684nmεmax=1.25×105Φf= 0.30τfl =1.8nsBrightness= 37.5Laser =633 –647nmFilterset =Cy®5  ATTO633Overview:HighfluorescenceyieldHighthermalandphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsStableatpH4–11Cationicdye,perchloratesaltMolarMass:652.2g/molATTO633DatasheetOpticalProperties:λex =629nmλem=657nmεmax=1.3×105Φf= 0.64τfl =3.2nsBrightness=83.2Laser =633 nmFilterset =Cy®5  ATTO594Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:1137g/molATTO594Datasheet OpticalProperties:λex =601 nmλem=627nmεmax=1.2×105Φf= 0.85τfl =3.5nsBrightness=102Laser =594nmFilterset =TexasRed®  ATTO565Overview:HighfluorescenceyieldHighthermalandphotostabilityGoodsolubilityinpolarsolventsExcellentsolubilityinwaterVerylittleaggregationRhodaminedyederivativeMolarMass:611g/molATTO565Datasheet OpticalProperties:λex =563nmλem=592nmεmax=1.2×105Φf= 0.9τfl =3.4nBrightness=10Laser =532nmFilterset = TRITC  ATTO488Overview:HighfluorescenceyieldHighphotostabilityVeryhydrophilicExcellentsolubilityinwaterVerylittleaggregationNewdyewithnetchargeof-1MolarMass:804g/mol ATTO488Datasheet  OpticalProperties:λex =501nmλem=523nmεmax=9.0×104Φf= 0.80τfl =4.1nsBrightness= 72Laser = 488 nmFilterset = FITC  ATTO390Overview:HighfluorescenceyieldLargeStokes-shift(89nm)GoodphotostabilityModeratelyhydrophilicGoodsolubilityinpolarsolventsCoumarinderivate,unchargedLowmolarmass:343.42g/mol ATTO390DatasheetOpticalProperties:λex =390nmλem=479nmεmax=2.4×104Φf= 0.90τfl =5.0nsBrightness= 21.6Laser =365or405nm APC(Allophycocyanin)Overview:HighquantumyieldLargephycobiliprotein6chromophorespermoleculeIsolatedfromredalgaeMolecularWeight:105kDaAPCDatasheet OpticalProperties:λex =650nmλem=660nmεmax=7.0×105Φf= 0.68Brightness= 476Laser =594or633 nmFilterset =Cy®5 StreptavidinProperties:Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinityMolecularweight:53kDaFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISAStreptavidinDatasheetBiotinProperties:BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinityMolarmass:244.31g/molFormula:C10H16N2O3SApplications:Westernblot,immunohistochemistry,andELISABiotinDatasheetHRP(HorserADIshperoxidase)Properties:EnzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatargetReadilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonorsCatalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproductsChemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproductsHighturnoverrateenablesrapidgenerationofastrongsignal44kDaglycoproteinExtinctioncoefficient:100(403nm)Applications:Westernblot,immunohistochemistry,andELISAHRPDatasheetAP(AlkalinePhosphatase)Properties:Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenolsCommonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligationCatalyzestheconversionof:Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproductsFluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproductsMolecularweight:140kDaApplications:Westernblot,immunohistochemistry,andELISAAPDatasheet R-PE(R-Phycoerythrin)Overview:BroadexcitationspectrumHighquantumyieldPhotostableMemberofthephycobiliproteinfamilyIsolatedfromredalgaeExcellentsolubilityinwaterMolecularWeight:250kDaR-PEDatasheet OpticalProperties:λex =565nmλem=575nmεmax=2.0×106Φf= 0.84Brightness=1.68x103Laser = 488to561nmFilterset = TRITC PropertiesStorageBufferPBSpH7.2,50%glycerol,0.09%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationProteinGPurifiedClonalityMonoclonalCloneNumberHyb-K41009IsotypeIgG2aSpecificityDetects90kDa.Thisisanalpha-specificproduct.CiteThisProductStressMarqBiosciencesCat#SMC-108,RRID:AB_854218CertificateofAnalysis1µg/mlofSMC-108wassufficientfordetectionofHSP90alphain20µgofheatshockedHeLacelllysatebycolorimetricimmunoblotanalysisusingGoatanti-mouseIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesHSP86Antibody,HSP89AAntibody,HSP90AAntibody,HSP90AA1Antibody,HSP90AlphaAntibody,HSPC1Antibody,HSPCAAntibody,HSPCAL3AntibodyResearchAreasCancer,HeatShockCellularLocalizationCytoplasm,MelanosomeAccessionNumberNP_001017963.2GeneID3320SwissProtP07900ScientificBackgroundHSP90isanabundantlyandubiquitouslyexpressedheatshockprotein.Itisunderstoodtoexistintwoprincipalformsαandβ,whichshare85%sequenceaminoacidhomology.ThetwoisoformsofHSP90,areexpressedinthecytosoliccompartment(1).Despitethesimilarities,HSP90αexistspredominantlyasahomodimerwhileHSP90βexistsmainlyasamonomer(2).Fromafunctionalperspective,HSP90participatesinthefolding,assembly,maturation,andstabilizationofspecificproteinsasanintegralcomponentofachaperonecomplex(3-6).FurThermore,HSP90ishighlyconservedbetweenspecies;having60%and78%aminoacidsimilaritybetweenmammalianandthecorrespondingyeastandDrosophilaproteins,respectively.HSP90isahighlyconservedandessentialstressproteinthatisexpressedinalleukaryoticcells.Despiteit’slabelofbeingaheat-shockprotein,HSP90isoneofthemosthighlyexpressedproteinsinunstressedcells(1–2%ofcytosolicprotein).Itcarriesoutanumberofhousekeepingfunctions–includingcontrollingtheactivity,turnover,andtraffickingofavarietyofproteins.MostoftheHSP90-regulatedproteinsthathavebeendiscoveredtodateareinvolvedincellsignaling(7-8).ThenumberofproteinsnowknowtointeractwithHSP90isabout100.Targetproteinsincludethekinasesv-Src,Wee1,andc-Raf,transcriptionalregulatorssuchasp53andsteroidreceptors,andthepolymerasesofthehepatitisBvirusandtelomerase(5).WhenboundtoATP,HSP90interactswithco-chaperonesCdc37,p23,andanassortmentofimmunophilin-likeproteins,formingacomplexthatstabilizesandprotectstargetproteinsfromproteasomaldegradation.Inmostcases,HSP90-interactingproteinshavebeenshowntoco-precipitatewithHSP90whencarryingoutimmunoadsorptionstudies,andtoexistincytosolicheterocomplexeswithit.Inanumberofcases,variationsinHSP90expressionorHSP90mutationhasbeenshowntodegradesignalingfunctionviatheproteinortoimpairaspecificfunctionoftheprotein(suchassteroidbinding,kinaseactivity)invivo.Ansamycinantibiotics,suchasgeldanamycinandradicicol,inhibitHSP90function(9).FormoreinformationvisitourHSP90ScientificResourceGuideathttp://www.HSP90.ca.References1.Nemoto,T.etal.(1997)J.BiolChem.272:26179-26187.2.Minami,Y,etal.(1991),J.BiolChem.266:10099-10103.3.ArlanderSJH,etal.(2003)JBiolChem.278:52572-52577.4.PearlH,etal.(2001)AdvProteinChem.59:157-186.5.NeckersL,etal.(2002)TrendsMolMed.8:S55-S61.6.PrattW,ToftD.(2003)ExpBiolMed.228:111-133.7.PrattW,ToftD.(1997)EndocrRev.18:306–360.8.PrattWB.(1998)ProcSocExptlBiolMed.217:420–434.9.WhitesellL,etal.(1994)ProcNatlAcadSciUSA.91:8324–8328.10.Nemoto,T.(1997)BiochemandMol.BioIntl.42(5):881-889.ProductImagesImmunohistochemistryanalysisusingMouseAnti-Hsp90alphaMonoclonalAntibody,CloneK41009(SMC-108).Tissue:coloncarcinoma.Species:Human.Fixation:Formalin.PrimaryAntibody:MouseAnti-Hsp90alphaMonoclonalAntibody(SMC-108)at1:5000for12hoursat4°C.SecondaryAntibody:BiotinGoatAnti-Mouseat1:2000for1houratRT.Counterstain:MayerHematoxylin(purple/blue)nuclearstainat200µlfor2minutesatRT.Localization:Inflammatorycells.Magnification:40x.WesternBlotanalysisofRatLysatesshowingdetectionofHsp90alphaproteinusingMouseAnti-Hsp90alphaMonoclonalAntibody,CloneK41009(SMC-108).Load:15µgprotein.Block:1.5%BSAfor30minutesatRT.PrimaryAntibody:MouseAnti-Hsp90alphaMonoclonalAntibody(SMC-108)at1:1000for2hoursatRT.SecondaryAntibody:SheepAnti-MouseIgG:HRPfor1houratRT.ImmunohistochemistryanalysisusingMouseAnti-Hsp90alphaMonoclonalAntibody,CloneK41009(SMC-108).Tissue:inflamedcolon.Species:Mouse.Fixation:Formalin.PrimaryAntibody:MouseAnti-Hsp90alphaMonoclonalAntibody(SMC-108)at1:5000for12hoursat4°C.SecondaryAntibody:BiotinGoatAnti-Mouseat1:2000for1houratRT.Counterstain:MayerHematoxylin(purple/blue)nuclearstainat200µlfor2minutesatRT.Localization:Inflammatorycells.Magnification:40x.ProductCitations(7)WesternBlotHeatshockprotein90ensuresefficientmumpsvirusreplicationbyassistingwithviralpolymerasecomplexformationKatoh,H.etal.(2017)JVirol.pii:JVI.02220-16.PubMedID:28053100ReactivityHumanApplications:WesternBlotEffectsofLong-TermExposureto60GHzMillimeter-WavelengthRadiationontheGenotoxicityandHeatShockProtein(Hsp)ExpressionofCellsDerivedfromHumanEye.Koyama,S.etal.(2016)IntJEnvironResPublicHealth.13(8).pii:E802.PubMedID:27509516ReactivityHumanApplications:WesternBlotTwentyFour-HourExposuretoa0.12THzElectromagneticFieldDoesNotAffecttheGenotoxicity,MorphologicalChanges,orExpressionofHeatShockProteininHCE-TCells.Koyama,S.etal.(2016)IntJEnvironResPublicHealth.13(8).pii:E802.PubMedID:27527204ReactivityHumanApplications:WesternBlotHeat-inducedexpressionoftheimmediate-earlygeneIER5anditsinvolvementintheproliferationofheat-shockedcells.Ishikawa,Y.andSakurai,H.(2014)FEBSJ.282(2):332-40.PubMedID:25355627ReactivityHumanApplications:WesternBlotHeatShockFactorHsf1CooperateswithErbB2(Her2/Neu)ProteintoPromoteMammaryTumOrigenesisandMetastasis.Xi,C.,Hu,Y.,Buckhaults,P.,Moskophidis,D.andMivechi,N.F.(2012)JBiolChem.287,35646-35657.PubMedID:22847003ReactivityHumanApplications:WesternBlotHeatShockProtein90α(Hsp90α)IsPhosphorylatedinResponsetoDNADamageandAccumulatesinRepairFoci.Quanz,M.etal.(2012)JBiolChem.287,8803-8815.PubMedID:22270370ReactivityHumanApplications:WesternBlotOtherCitationsAssociationsofHSP90ClientProteinsinHumanBreastCancer.Shipp,C.,Watson,K.,andJones,G.L.(2011)AnticancerResearch.31(6):2095-2101.PubMedID:21737627ReactivityHumanApplications:Immunoprecipitation ATTO565Overview:HighfluorescenceyieldHighthermalandphotostabilityGoodsolubilityinpolarsolventsExcellentsolubilityinwaterVerylittleaggregationRhodaminedyederivativeMolarMass:611g/molATTO565Datasheet OpticalProperties:λex =563nmλem=592nmεmax=1.2×105Φf= 0.9τfl =3.4nBrightness=10Laser =532nmFilterset = TRITC