Overview: Product Name iNOS Antibody DescriptionRabbit Anti-Human iNOS Polyclonal Species Reactivity Human Applications WB, IHC, ICC/IF, ELISA Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user. Host Species Rabbit Immunogen Species Human Immunogen Synthesized peptide derived from human iNOS. Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated Properties Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide Storage Temperature -20ºC Shipping Temperature Blue Ice or 4ºC Purification Affinity Purified Clonality Polyclonal Isotype IgG Specificity Detects endogenous levels of total iNOS protein. Cite This Product StressMarq Biosciences Cat# SPC-1325, RRID: AB_2713637 Certificate of Analysis A 1:1000 dilution of SPC-1325 was sufficient for detection of iNOS in 10 µg of NIH/3T3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. Biological Description Alternative Names HEP-NOS antibody, Hepatocyte NOS antibody, HEPNOS antibody, inducible antibody, Inducible nitric oxide synthase antibody, Inducible NO synthase antibody, Inducible NOS antibody, iNOS antibody, MAC NOS antibody, Macrophage NOS antibody, Nitric oxide synthase 2 inducible antibody, Nitric oxide synthase 2 inducible macrophage antibody, nitric oxide synthase 2A (inducible, hepatocytes) antibody, Nitric oxide synthase antibody, Nitric oxide synthase inducible antibody, nitric oxide synthase, macrophage antibody, NOS 2 antibody, NOS antibody, Nos II antibody, NOS type II antibody, nos2 antibody, NOS2_HUMAN antibody, NOS2A antibody, NOS2A, Inducible, Hepatocyte antibody, Peptidyl-cysteine S-nitrosylase NOS2 antibody Cellular Localization Cytoplasm, Nucleus, Peroxisome Accession Number NP_000616.3 Gene ID 4843 Swiss Prot P35228 Scientific Background Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. References 1. Knowles RG and Moncada S. (1994) Biochem. J. 298 (2): 249–58. 2. Burger DE. et al. (2009) Circulation 120 (14): 1345–54. 3. Taylor BS. (1997) Arch Surg 132 (11): 1177–83. Product Images Immunohistochemistry analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide. Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Tissue: HepG2 cells. Species: Human. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:100. The image on the right is treated with the synthesized peptide. Western blot analysis of Mouse NIH/3T3 cell lysates showing detection of ~131kDa iNOS protein using Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325). Lane 1: Mouse NIH/3T3. Lane 2: Mouse NIH/3T3 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-iNOS Polyclonal Antibody (SPC-1325) at 1:1000. Predicted/Observed Size: ~131kDa. Product Citations (0) Currently there are no citations for this product. PE/ATTO 594PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.Overview:High fluorescence yieldHigh photostabilityVery hydrophilicExcellent solubility in waterVery little aggregationPE/ATTO 594 DatasheetOptical Properties:λex = 535 nmλem = 627 nmLaser = 488 to 561 nm