Overview: Product Name Ki67 Antibody DescriptionRabbit Anti-Human Ki67 Polyclonal Species Reactivity Human Applications IHC, ICC/IF, ELISA Antibody Dilution IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user. Host Species Rabbit Immunogen Species Human Immunogen Synthesized peptide derived from C-terminal of human ki67. Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated Properties Storage Buffer PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide Storage Temperature -20ºC Shipping Temperature Blue Ice or 4ºC Purification Affinity Purified Clonality Polyclonal Isotype IgG Specificity Detects endogenous levels of total ki67protein. Cite This Product StressMarq Biosciences Cat# SPC-1327, RRID: AB_2713673 Certificate of Analysis A 1:100 dilution of SPC-1327 was sufficient for detection of Ki67 in human lung carcinoa tissue using Goat Anti-Rabbit IgG as the secondary antibody. Biological Description Alternative Names Antigen identified by monoclonal antibody Ki 67 antibody, Antigen KI-67 antibody, Antigen KI67 antibody, KI67_HUMAN antibody, KIA antibody, Marker of proliferation Ki-67 antibody, MIB 1 antibody, MIB antibody, MKI67 antibody, PPP1R105 antibody, Proliferation related Ki 67 antigen antibody, Protein phosphatase 1 regulatory subunit 105 antibody, RP11-380J17.2 antibody Cellular Localization Chromosome, Nucleus Accession Number NP_002408.3 Gene ID 4288 Swiss Prot P46013 Scientific Background Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore, it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. Due to its association with cell divison process, Ki-67 is routinely used as cellular proliferation marker of solid tumors as well as certain hematological malignancies, and a correlation has been demonstrated between Ki-67 index and the histopathological grade of cancers. References 1. Schonk DM. et al. (1989) Hum. Genet. 83 (3): 297–9. 2. Bullwinkel J. et al. (2006) J. Cell. Physiol. 206 (3): 624–35. 3. Rahmanzadeh R. et al. (2007) Cell Prolif. 40 (3): 422–30. 4. Scholzen T and Gerdes J (2000) J. Cell. Physiol. 182 (3): 311–22. Product Images Immunohistochemistry analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Lung Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide. Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Ki67 Polyclonal Antibody (SPC-1327) at 1:100. The image on the right is treated with the synthesized peptide. Product Citations (0) Currently there are no citations for this product. ATTO 655Overview:High fluorescence yieldHigh thermal and photostabilityExcellent ozone resistanceQuenched by electron donorsVery hydrophilicGood solubility in polar solventsZwitterionic dyeMolar Mass: 634 g/molATTO 655 DatasheetOptical Properties:λex = 663 nmλem = 684 nmεmax = 1.25×105Φf = 0.30τfl = 1.8 nsBrightness = 37.5Laser = 633 – 647 nmFilter set = Cy®5