首页 > StressMarq > StressMarq/Anti-PCNA Antibody/SPC-1307D-RPE/100-µl

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StressMarq/Anti-PCNA Antibody/SPC-1307D-RPE/100-µl

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SPC-1307D-RPE

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Overview:ProductNamePCNAAntibodyDescriptionRabbitAnti-HumanPCNAPolyclonalSpeciesReactivityHuman,Mouse,RatApplicationsWB,IHC,ELISAAntibodyDilutionWB(1:1000),IHC(1:100),ELISA(1:5000);optimaldilutionsforassaysshouldbedeterminedbytheuser.HostSpeciesRabbitImmunogenSpeciesHumanImmunogenSynthesizedpeptidederivedfromhumanPCNA.ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,UnconjugatedPropertiesStorageBufferPBSpH7.4,50%glycerol,150mMNaCl,0.02%sodiumazideStorageTemperature-20ºCShippingTemperatureBlueIceor4ºCPurificationAffinityPurifiedClonalityPolyclonalIsotypeIgGSpecificityDetects~29kDa,endogenouslevelsoftotalPCNAprotein.CiteThisProductStressMarqBiosciencesCat#SPC-1307,RRID:AB_2713313CertificateofAnalysisA1:1000dilutionofSPC-1307wassufficientfordetectionofPCNAin10µgofHepG2celllysatesbyECLimmunoblotanalysisusingGoatAnti-RabbitIgG:HRPasthesecondaryantibody.BIOLOGicalDescriptionAlternativeNamesATLD2Antibody,cb16Antibody,CyclinAntibody,DNApolymerasedeltaauxiliaryproteinAntibody,etID36690.10Antibody,fa28e03Antibody,fb36g03Antibody,HGCN8729Antibody,MGC8367Antibody,Mutagen-sensitive209proteinAntibody,OTTHUMP00000030189Antibody,OTTHUMP00000030190Antibody,PCNAAntibody,Pcna/cyclinAntibody,PCNA_HUMANAntibody,PCNARAntibody,PolymerasedeltaaccessoryproteinAntibody,ProliferatingcellnuclearantigenAntibody,wu:fa28e03Antibody,wu:fb36g03AntibodyaCellularLocalizationNucleusAccessionNumberNP_872590.1GeneID5111SwissProtP12004ScientificBackgroundProliferatingcellnuclearantigen(PCNA)isaDNAclampthatactsasaprocessivityfactorforDNApolymeraseδineukaryoticcellsandisessentialforreplication.Itisa36kDamoleculethatishighlyconservedbetweenspecies.ThisproteinisanauxiliaryproteinofDNApolymerasedeltaandisinvolvedinthecontrolofeukaryoticDNAreplicationbyincreasingthepolymerase"sprocessibilityduringelongationoftheleADIngstrand.References1.MoldovanGL.etal.(2007)Cell129(4):665–79.2.WarbrickE.etal.(1998)BioEssays20(3):195–9.3.GilljamKM.etal.(2009)TheJournalofCellBiology186(5):645–54.ProductImagesImmunohistochemistryanalysisusingRabbitAnti-PCNAPolyclonalAntibody(SPC-1307).Tissue:BreastCarcinomaTissue.Species:Human.Fixation:Formalinfixedparaffin-embedded.PrimaryAntibody:RabbitAnti-PCNAPolyclonalAntibody(SPC-1307)at1:100.Theimageontherightistreatedwiththesynthesizedpeptide.WesternblotanalysisofHumanHepG2celllysatesshowingdetectionof~29kDaPCNAproteinusingRabbitAnti-PCNAPolyclonalAntibody(SPC-1307).Lane1:HumanHepG2cells.Lane2:HumanHepG2cellstreatedwiththeimmunizingpeptide.PrimaryAntibody:RabbitAnti-PCNAPolyclonalAntibody(SPC-1307)at1:1000.Predicted/ObservedSize:~29kDa.ProductCitations(0)Currentlytherearenocitationsforthisproduct. R-PE(R-Phycoerythrin)Overview:BroadexcitationspectrumHighquantumyieldPhotostableMemberofthephycobiliproteinfamilyIsolatedfromredalgaeExcellentsolubilityinwaterMolecularWeight:250kDaR-PEDatasheet OpticalProperties:λex =565nmλem=575nmεmax=2.0×106Φf= 0.84Brightness=1.68x103Laser = 488to561nmFilterset = TRITC

StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。