产品说明
DescriptionLeavesomefeedback! TestedandCertifiedforinvivoimaging(See"LuciferinFAQ"inadditionalinformation)Luciferinisacommonbioluminescentreporterusedforinvivoimagingoftheexpressionofluciferase.ThiswatersolublesubstrateforthefireflyluciferaseenzymeutilizesATPandMg2+ascofactorstoemitacharacteristicyellow-greenemissioninthepresenceofoxygen,whichshiftstoredlightinvivoat37°C.ThroughtheutilizationofATP,thereactioncanbefurtherusedtoindicatethepresenceofenergyorlifeinordertofunctionasalife-deathstain.Luciferinisacommonreagentusedthroughoutthebiotechnologyfieldandspecificallyforinvivoimaging.Luciferaselabeledtumorcells,stemcellsorinfectiousdiseasesareofteninoculatedintoresearchanimalssuchasratsormiceforinvestigation.Theinjectionofluciferinallowsforthereal-time,noninvasivemonitoringofdiseaseprogressionand/ordrugefficacyinthesemodelsystemsthroughBioluminescenceImaging(BLI).Luciferinisalsocommonlyusedforinvitroresearch,includingluciferaseandATPassays,genereporterassays,highthroughputsequencingandvariouscontaminationassays.FireflyLuciferinisidenticaltoBeetleLuciferin.(See"AdditionalInformation"forstructures.)ProductSpecifications:D-Luciferin,PotassiumSalt,4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylicacidpotassiumsalt,MOLECULARBIOLOGYGRADE(PremiumPure)Formula:KC11H7N2O3S2MW:318.42g/molColor:LightyellowpowderPurity:>99%pure.(QualityverifiedbynineindependentcriteriaincludingHPLCandFTIR.)Storage/Handling:Storedesiccatedat-20°C.Protectfromlight.PubChemChemicalID:44134804
D-荧光素钾盐/钠盐体内使用手册--Goldbio介绍
荧光素是一种常用的荧光素酶表达的活体成像生物发光报告子。以ATP和Mg2+作为辅因子,有氧条件下荧火虫荧光素酶与水溶性底物反应发出一种特有的黄绿色发射光,在37℃活体内转换为红光。该反应通过消耗ATP来指示是否存在能量或生命的存在。
D-荧光素在生物技术领域是一种常用的试剂,尤其在活体成像方面。荧光素酶被接种在研究动物中比如大鼠,小鼠,用来标记肿瘤细胞,干细胞或传染病。通过生物荧光成像这个模式系统中D-荧光素的注射可以用来实时,非侵入性监测疾病的进程或药物效率。
产品具体信息
D-荧光素钾盐
4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt
KC11H7N2O3S2
分子量:318.42 g/mol
D-荧光素钠盐
4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt
NaC11H7N2O3S2.H2O
分子量:320.32 g/mol
储存:-20℃避光保存。
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