产品说明
GelRed™ is an ultra-sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed™ Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.6X GelRed™ Prestain Loading Buffer with Orange Tracking Dye contains an orange electrophoresis tracking dye that run at approximately 50 bp in a 1% agarose gel. For larger tracking dyes, see Catalog # G-730 (6X GelRed™ Prestain Loading Buffer with Blue Tracking Dyes).GelRed™ and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed™ is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed™ is compatible with downstream applications such as sequencing and cloning. GelRed™ is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.A series of safety tests have confirmed that GelRed™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal.FeaturesSafer than Ethidum BromideShown by the Ames test and other tests to be nonmutagenic and noncytotoxic Easy disposalPassed environmental safety tests for direct disposal down the drain or in regular trash Ultra-sensitiveHigher sensitivity than EtBr and SYBR® Safe Extremely stableAvailable in water, stable at room temperature for long-term storage and microwavable Simple to useVery simple procedures for precast or post-electrophoresis gelCompatible with a standard UV transilluminatorReplaces EtBr with no optical setting change Compatible with downstream applicationsCompatible with gel purification, restriction digest, sequencing and cloning Storage/Handling: Store at 4°C. Protect from light.Productmay be shipped at ambient temperature without reducing performance.Please place at the recommended storage conditions upon receipt of theproduct.GelRed and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. SYBR is trademark of Molecular Probes, Inc.
D-荧光素钾盐/钠盐体内使用手册--Goldbio介绍
荧光素是一种常用的荧光素酶表达的活体成像生物发光报告子。以ATP和Mg2+作为辅因子,有氧条件下荧火虫荧光素酶与水溶性底物反应发出一种特有的黄绿色发射光,在37℃活体内转换为红光。该反应通过消耗ATP来指示是否存在能量或生命的存在。
D-荧光素在生物技术领域是一种常用的试剂,尤其在活体成像方面。荧光素酶被接种在研究动物中比如大鼠,小鼠,用来标记肿瘤细胞,干细胞或传染病。通过生物荧光成像这个模式系统中D-荧光素的注射可以用来实时,非侵入性监测疾病的进程或药物效率。
产品具体信息
D-荧光素钾盐
4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt
KC11H7N2O3S2
分子量:318.42 g/mol
D-荧光素钠盐
4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt
NaC11H7N2O3S2.H2O
分子量:320.32 g/mol
储存:-20℃避光保存。
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