DESCRIPTION:HumanrecombinantEZH1(residues2-747;GenbankAccession#NM_001991;MW=88kDa)incomplexwithhumanrecombinantsAEBP2(2-517;NM_001114176;57kDa),EED(2-441;NM_003797;51kDa),RbAp48(2-425;NM_005610;51kDa)andSUZ12(2-739;NM_015355;86kDa).TotalcomplexMWis333.4kDa.Allproteinsarefull-length(residue2throughC-terminus)andco-expressedinaninsectcell/baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS-adenosyl-L-methionine(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3(H3K27).Duringdevelopment,PolycombRepressiveComplex2(PRC2)istheprincipalmethyltransferaseresponsIBLeforgeneratingtrimethylatedhistoneH3lysine-27(H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,functionsasthecatalyticsubunitofPRC21,6,butcanbereplacedbythehomologousEZH17,whichislesscatalyticallyactive8,9,althoughPRC2-Ezh1hasstrongrepressiveactivityontranscriptionindependentofitsmethyltransferaseactivity9.EZH1actstopreservetherepressiveH3K27me3markinembryonicstemcells,complementingtheroleofEZH28.MultiplerecentresultssuggestimportantrolesforEZH1indevelopmentalandhomeostaticprocesses,includingmaintenanceoftheleft-rightaxis10,hairfolliclehomeostasisandwoundrepair11,skeletalmuscledifferentiation12andadulthematopoiesis13.ACCESSION#:NM_001991,NM_001114176,NM_003797,NM_005610,NM_015355UniprotLinkINCLUDESAMINOACIDS:EZH12-747,AEBP22-517,EED2-441,RbAp482-425,SUZ122-739;Allfull-lengthTAG(S):N-terminalFlag-tagonEED,allothersN-terminalHis-tagMW:333.4kDaEXPRESSIONSYSTEM:Insectcell/BaculovirusSUPPLIEDAS:__µMEZH1Complex,asdefinedabove,(___µg/µltotalprotein)in20mMTris-HCl,pH7.9,150mMNaCl,2mMMgCl2,2mMDTT,20%glycerol(w/v),0.01%NP-40.STORAGE:-70°C.Thawquicklyandstoreonicebeforeuse.Theremaining,unused,undilutedenzymeshouldbesnapfrozen,forexampleinadry/iceethanolbathorliquidnitrogen.Minimizefreeze/thawsifpossible,butverylowvolumealiquots(<5µl)orstorageofdilutedenzymeisnotrecommended.ViewProductDataSheetSubstantialDiscountsAvailableonBulkQuantitiesWealsoofferassayservicesforEZH1.
Reaction Biology描述:
人类重组EZH2incomplexwithhumanrecombinantsEED.TotalcomplexMWis228.7kDa.Allproteinsarefull长度(residue2throughC末端)也是全美-expressedinaninsectcell / baculovirusexpressionsystem.TheEEDsubunitincorporatesanN-terminalStrepandFlag-tagandallothersincludeanN-terminalHis-tag.CatalyzesthetransferofmethylgroupsfromS腺苷-L-甲硫氨酸(SAM)tothee-aminofunctionofproteinL-lysineresidues,specificallylysine-27ofhistoneH3( H3K27)。在开发过程中,Polycomb Repressive Complex 2(PRC2)是主要的甲基转移酶对IBL的反应eforgeneratingtrimethylatedhistoneH3lysine-27(的H3K27me3),anepigeneticmarkessentialforprogrammedrepressionofgeneexpression1-5.EZH2,whichincludesaSETmethyltransferasedomain,isthecatalyticsubunitofPRC21,6.ThecoreofthecatalyticcomplexincludesEZH2,EED,SUZ12andRbAp48,whileadditionofAEBP2significantlyenhancesthemethyltransferaseactivityofthecomplex(> 3×)6.EZH2isoverexpressedinawiderangeofhumancancersanditsoverexpressioncancorrelatewithtumorprogression,increasedmetastasisandpoorprognosis(seereview7).DepletionofEZH2and / orotherPRC2componentscaninhibitgrowthorinduceapoptosisincancercells6,8-10.Consequently,EZH2isconsideredapromisingtargetforthedevelopmentofanti -癌症疗法11
存取编号: EZH2NM_001203247,EEDNM_003797,SUZ12NM_015355
包括酰胺酸:EZH22-746,EED2-441,SUZ122-739
标记(S):EEDhasanN末端链霉菌标记标签,其他所有N末端His标签
分子量:228.7kDa
EXPRESSIONSYSTEM :昆虫细胞/杆状病毒
SUPPLIEDAS:Solutionofpurifiedrecombinantproteinin20mMTris盐酸,pH8,150mMNaCl,2mMMgCl 2,2mMDTT,20%甘油(w / v),0.01%NP-40asdeterminedbyOD 280。
储存:-70°C。在使用前应迅速融化并储存。应保留未使用的未稀释蛋白质,例如干燥/冰乙醇液中的氮。尽可能减少冻融/解冻,但不建议使用稀释体积太小(<5µl)的稀释蛋白质。